“…Fungal GH5 and GH74 XEGs have also been shown to display an endo-type of hydrolysis of the xyloglucan backbone, cleaving at the reducing end side of unsubstituted glucosyl units like the GH12 XEGs ( Master et al., 2008 ; Matsuzawa et al., 2020 ; Vitcosque et al., 2016 ). However, in contrast to the GH12 XEGs, the GH5 and GH74 XEGs display a processive mode of action ( Arnal et al., 2019 ; Berezina et al., 2017 ; Desmet et al., 2007 ; Martinez-Fleites et al., 2006 ; Matsuzawa et al., 2020 ; Yaoi and Mitsuishi, 2002 ). As a result, from the early stage of hydrolysis, XXXG- and XXLG-building blocks are released by GH74 XEGs, which can even be further degraded into XX, XG, and LG ( Arnal et al., 2019 ; Attia et al., 2016 ; Desmet et al., 2007 ; Yaoi and Mitsuishi, 2002 ).…”