Identification and characterization of virus-encoded circular RNAs in host cells

Chen, Shuting; Zheng, Jie; Zhang, Bingyue; Tang, Xiaozhi; Cun, Yewei; Wu, Tao; Ma, Ting; Cheng, Jianhua; Yu, Zuoren; Wang, Haiyun

doi:10.1099/mgen.0.000848

Cited by 5 publications

(2 citation statements)

References 83 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They may affect cellular processes such as the IFN responses, the unfolded protein response, and mitochondrial antiviral signaling [99]. EBV can generate circR-NAs, including spliced transcripts in the BART region [125] and have been found in clinical samples from EBV-related cancers [126]. The function of viral circRNAs is unknown, though their similar structure to the host circRNAs implies that they have equivalent roles in restricting viral overproliferation as a means of immune escape [127].…”

Section: Ebv-derived Ncrnasmentioning

confidence: 99%

Epigenetic reprograming in myalgic encephalomyelitis/chronic fatigue syndrome: A narrative of latent viruses

Apostolou,

Rosén

2024

J Intern Med

View full text Add to dashboard Cite

Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a chronic disease presenting with severe fatigue, post‐exertional malaise, and cognitive disturbances—among a spectrum of symptoms—that collectively render the patient housebound or bedbound. Epigenetic studies in ME/CFS collectively confirm alterations and/or malfunctions in cellular and organismal physiology associated with immune responses, cellular metabolism, cell death and proliferation, and neuronal and endothelial cell function. The sudden onset of ME/CFS follows a major stress factor that, in approximately 70% of cases, involves viral infection, and ME/CFS symptoms overlap with those of long COVID. Viruses primarily linked to ME/CFS pathology are the symbiotic herpesviruses, which follow a bivalent latent–lytic lifecycle. The complex interaction between viruses and hosts involves strategies from both sides: immune evasion and persistence by the viruses, and immune activation and viral clearance by the host. This dynamic interaction is imperative for herpesviruses that facilitate their persistence through epigenetic regulation of their own and the host genome. In the current article, we provide an overview of the epigenetic signatures demonstrated in ME/CFS and focus on the potential strategies that latent viruses—particularly Epstein–Barr virus—may employ in long‐term epigenetic reprograming in ME/CFS. Epigenetic studies could aid in elucidating relevant biological pathways impacted in ME/CFS and reflect the physiological variations among the patients that stem from environmental triggers, including exogenous viruses and/or altered viral activity.

show abstract

Section: Ebv-derived Ncrnasmentioning

confidence: 99%

Epigenetic reprograming in myalgic encephalomyelitis/chronic fatigue syndrome: A narrative of latent viruses

Apostolou,

Rosén

2024

J Intern Med

View full text Add to dashboard Cite

show abstract

“…However, for non-model organisms, removal of host RNAs remains challenging. Ribonuclease R (RNase R), which is a 3' to 5' exonuclease that digests linear RNAs 11 , has recently been used to detect circular RNAs derived from cellular transcripts or viral genes 12 – 14 . We applied RNase R treatment to reduce most linear cellular RNAs and enrich viral genomes containing double-stranded (ds) RNA structures at their 3'-ends.…”

Section: Introductionmentioning

confidence: 99%

Expanding diversity of bunyaviruses identified in mosquitoes

Orba,

Abu,

Chambaro

et al. 2023

Sci Rep

View full text Add to dashboard Cite

Mosquitoes interact with various organisms in the environment, and female mosquitoes in particular serve as vectors that directly transmit a number of microorganisms to humans and animals by blood-sucking. Comprehensive analysis of mosquito-borne viruses has led to the understanding of the existence of diverse viral species and to the identification of zoonotic arboviruses responsible for significant outbreaks and epidemics. In the present study on mosquito-borne bunyaviruses we employed a broad-spectrum RT-PCR approach and identified eighteen different additional species in the Phenuiviridae family and also a number of related but unclassified bunyaviruses in mosquitoes collected in Zambia. The entire RNA genome segments of the newly identified viruses were further analyzed by RNA sequencing with a ribonuclease R (RNase R) treatment to reduce host-derived RNAs and enrich viral RNAs, taking advantage of the dsRNA panhandle structure of the bunyavirus genome. All three or four genome segments were identified in eight bunyavirus species. Furthermore, L segments of three different novel viruses related to the Leishbunyaviridae were found in mosquitoes together with genes from the suspected host, the Crithidia parasite. In summary, our virus detection approach using a combination of broad-spectrum RT-PCR and RNA sequencing analysis with a simple virus enrichment method allowed the discovery of novel bunyaviruses. The diversity of bunyaviruses is still expanding and studies on this will allow a better understanding of the ecology of hematophagous mosquitoes.

show abstract

BmNPV circular RNA-encoded peptide VSP39 promotes viral replication

Zhang

Shen

et al. 2023

International Journal of Biological Macromolecules

View full text Add to dashboard Cite

Identification and characterization of virus-encoded circular RNAs in host cells

Cited by 5 publications

References 83 publications

Epigenetic reprograming in myalgic encephalomyelitis/chronic fatigue syndrome: A narrative of latent viruses

Epigenetic reprograming in myalgic encephalomyelitis/chronic fatigue syndrome: A narrative of latent viruses

Expanding diversity of bunyaviruses identified in mosquitoes

BmNPV circular RNA-encoded peptide VSP39 promotes viral replication

Contact Info

Product

Resources

About