The objective of this study is to develop a method for identifying and discriminating ten potato varieties by combining machine vision and artificial neural network methods. The potato varieties include Agria, Emrad. A total number of 72 characteristic parameters specifying color, textural and morphological features are found among these varieties. By using principal component analysis (PCA), 16 principal features are selected for identifying and discriminating potato varieties. The data obtained from image processing were classified using linear discriminant analysis and nonlinear artificial neural network method. The accuracy of discriminant analysis were 73.3%, 93.3%, 73.3%, 40%, 73.3%, 73.3%, 66.7%, 80%, 40% and 53.3%, respectively for the varieties used in this study. The classification accuracy was improved by 100% for all the varieties using neural network analysis and the Correct Classification Ratio (CCR) was 100% using this method.A c c e p t e d M a n u s c r i p t 2 It is revealed from the results that machine vision technique and neural network analysis could identify potato varieties with acceptable accuracy.