Identification and cloning of three novel human G protein-coupled receptor genes GPR52, ΨGPR53 and GPR55: GPR55 is extensively expressed in human brain

The orphan receptor GPR55 is a novel cannabinoid receptor

“…The sequence of the human gene was similar but not identical to that already described (Sawzdargo et al, 1999), however it was consistent with the human genome sequence. The sequence of all clones isolated differed in that there was a nucleotide insertion and deletion at positions 393 and 427 respectively, resulting in a frame shift of the translated sequence, consequently changing 11 amino acids at the predicted junction of intracellular loop 2 and transmembrane helix 4 (Figure 1).…”

“…Both the rat and mouse sequences are consistent with the human genome sequence in the region of the intracellular loop 2-transmembrane helix 4 region rather than the published sequence (Sawzdargo et al, 1999) containing the insertion and deletion (Figure 1). Despite GPR55, a novel cannabinoid receptor E Ryberg et al the low level of identity between the human and rodent forms of GPR55, the genomic linkage confirms that the rodent genes are orthologues of the human gpr55.…”

“…The sequence of all clones isolated differed in that there was a nucleotide insertion and deletion at positions 393 and 427 respectively, resulting in a frame shift of the translated sequence, consequently changing 11 amino acids at the predicted junction of intracellular loop 2 and transmembrane helix 4 (Figure 1). Since we could find no evidence for the existence of the previously published sequence, we concluded that the difference originated from a sequencing error by the authors (Sawzdargo et al, 1999).…”

“…The putative positions of the transmembrane regions (TM1-7), extracellular loops (EC1-3) and intracellular loops (EC1-3) are shown. The amino-acid differences in the previously published sequence (Sawzdargo et al, 1999) for human GPR55 at the IC2/TM4 boundary are shown above the sequences.…”

A solid-phase method for the extraction and measurement of anandamide from multiple human biomatrices

“…The endocannabinoid, anandamide (N-arachidonoylethanolamine; AEA), is an endogenous, unsaturated fatty acid derivative that was first identified in porcine brain [1] and has since been characterised as a ligand for the cannabinoid receptors, CB1 and CB2 [2] and the orphan receptors GPR55 [3] and GPR119 [4]. AEA also binds to the Ca 2+ -dependent membrane transporter and vaniloid receptor 1 (TRPV1) [5].…”