Identification and Comparative Expression Profiles of Chemoreception Genes Revealed from Major Chemoreception Organs of the Rice Leaf Folder, Cnaphalocrocis medinalis (Lepidoptera: Pyralidae)

Zeng, Fangfang; Zhao, Zhen-Fei; Yan, Miao-Jun; Zhou, Wen; Zhang, Zan; Zhang, Aijun; Lü, Zhongxian; Wang, Man‐Qun

doi:10.1371/journal.pone.0144267

Cited by 37 publications

(64 citation statements)

References 62 publications

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“…Our 10 LstiCSPs were primarily expressed in the legs and antennae of the adults, which was similar to the results of other Lepidoptera [9, 21, 31, 33, 43, 45]. But LstiCSP9 was mainly expressed in larvae.…”

Section: Discussionsupporting

confidence: 88%

Identification of candidate chemosensory genes by transcriptome analysis in Loxostege sticticalis Linnaeus

Wei

Zhang

et al. 2017

PLoS ONE

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Loxostege sticticalis Linnaeus is an economically important agricultural pest, and the larvae cause great damage to crops, especially in Northern China. However, effective and environmentally friendly chemical methods for controlling this pest have not been discovered to date. In the present study, we performed HiSeq2500 sequencing of transcriptomes of the male and female adult antennae, adult legs and third instar larvae, and we identified 54 candidate odorant receptors (ORs), including 1 odorant receptor coreceptor (Orco) and 5 pheromone receptors (PRs), 18 ionotropic receptors (IRs), 13 gustatory receptors (GRs), 34 odorant binding proteins (OBPs), including 1 general odorant binding protein (GOBP1) and 3 pheromone binding proteins (PBPs), 10 chemosensory proteins (CSPs) and 2 sensory neuron membrane proteins (SNMPs). The results of RNA-Seq and RT-qPCR analyses showed the expression levels of most genes in the antennae were higher than that in the legs and larvae. Furthermore, PR4, OR1-4, 7–11, 13–15, 23, 29–32, 34, 41, 43, 47/IR7d.2/GR5b, 45, 7/PBP2-3, GOBP1, OBP3, 8 showed female antennae-biased expression, while PR1/OBP2, 7/IR75d/CSP2 showed male antennae-biased expression. However, IR1, 7d.3, 68a/OBP11, 20–22, 28/CSP9 had larvae enriched expression, and OBP15, 17, 25, 29/CSP5 were mainly expressed in the legs. The results shown above indicated that these genes might play a key role in foraging, seeking mates and host recognition in the L. sticticalis. Our findings will provide the basic knowledge for further studies on the molecular mechanisms of the olfactory system of L. sticticalis and potential novel targets for pest control strategies.

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Section: Discussionsupporting

confidence: 88%

Identification of candidate chemosensory genes by transcriptome analysis in Loxostege sticticalis Linnaeus

Wei

Zhang

et al. 2017

PLoS ONE

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“…Previously, Zeng et al () identified 29 OR s from C. medinalis antennae, including four PR s, one ORco and 24 conventional OR s ( CmedOR1 to CmedOR24 ). In the present study, we screened a total of 46 candidate ORs from the antennal transcriptome dataset, including 29 novel ones.…”

Section: Resultsmentioning

confidence: 99%

Transcriptome sequencing reveals abundant olfactory genes in the antennae of the rice leaffolder, Cnaphalocrocis medinalis (Lepidoptera: Pyralidae)

Liu

Wang

Zhang

et al. 2017

Entomological Science

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The rice leaffolder, Cnaphalocrocis medinalis Guenée, is an important lepidopteran pest of rice in Asia. Insect olfactory proteins, including olfactory receptors (ORs), ionotropic receptors (IRs), odorant‐binding proteins (OBPs), chemosensory proteins (CSPs) and sensory neuron membrane proteins (SNMPs), are responsible for perception of sex pheromones and host plant volatiles, and thus regulate insect behavior. In the present study, transcriptome sequencing was conducted for C. medinalis antennae to identify genes involved in olfaction. A total of 45800 unigenes were assembled from the transcriptome dataset. Of these, 19696 (43.0%) unigenes were annotated by searching against the NCBI non‐redundant database. Functional classification of these unigenes were also conducted by using the Gene Ontology (GO) and Cluster of Orthologous Groups (COG) databases. We identified 90 putative olfactory genes (including 37 novel ones): 46 ORs, 15 IRs, 12 OBPs, 15 CSPs and two SNMPs. BLASTX best hit results indicated that these genes were most identical to their respective orthologs from other lepidopteran insects. Quantitative reverse transcription‐PCR (qRT‐PCR) assays were performed to investigate the expression profiles of newly identified OR genes. All of the OR genes were antennae‐specific or antennae‐enriched. Of these, CmedOR28 and CmedOR31 were mainly expressed in male antennae, while CmedOR27 and CmedOR32 were enriched in female antennae. Our results establish a solid foundation for future functional studies of these genes.

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“…The rice leaf folder Cnaphalocrocis medinalis (Lepidoptera: Pyralidae) is one of the most destructive pests damaging rice in Asia (Barrion et al ., ; Rice and Choo, ). In a previous study, 29 CSP‐encoding genes were identified in the C. medinalis transcriptome (Zeng et al ., , ). To identify the CSPs involved in chemical reception and attempt to utilize them as targets for screening behaviourally active compounds, we choose an antenna‐biased CSP gene encoding CmedCSP33 for the following experiments: (1) cloning, expression and purification of CmedCSP33 in vitro ; (2) screening of potential behaviourally active compounds based on interactions between CmedCSP33 and 32 rice volatiles in vitro using fluorescence competitive binding, fluorescence quenching, CD spectra, homology modelling and molecular docking; and (3) verifying the behavioural responses of C. medinalis to candidate compounds in vivo using an H‐tube olfactometer.…”

Section: Introductionmentioning

confidence: 99%

Chemosensory proteins used as target for screening behaviourally active compounds in the rice pest Cnaphalocrocis medinalis (Lepidoptera: Pyralidae)

Duan

Wang

2018

Insect Molecular Biology

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Reverse chemical ecology based on insect functional odorant binding proteins has been extensively studied to screen behaviourally active compounds, whereas chemosensory proteins (CSPs), which are reportedly involved in olfactory chemical reception and could serve as molecular targets remain unclear. In the present study, two behaviourally active compounds for Cnaphalocrocis medinalis, a serious pest of rice in Asia, were successfully screened via an antenna-biased CSP, CmedCSP33. Fluorescence competitive binding assays showed that CmedCSP33 could bind to seven out of 32 rice volatiles. Fluorescence quenching experiments revealed that CmedCSP33 forms a stable complex with nerolidol and β-ionone, and circular dichroism (CD) spectra demonstrated that these two compounds cause conformational changes in CmedCSP33. Furthermore, H-tube olfactometer bioassays showed that C. medinalis displayed prominent attractant responses to nerolidol and prominent repellent responses to β-ionone. Additionally, binding assays and CD spectra at different pH values implied that extensive conformational changes may be a general feature of CSPs for triggering the subsequent chemical transduction. Overall, our findings provide evidence for the involvement of CSPs in olfactory perception, and a protocol for effectively screening behaviourally active compounds.

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Identification and Comparative Expression Profiles of Chemoreception Genes Revealed from Major Chemoreception Organs of the Rice Leaf Folder, Cnaphalocrocis medinalis (Lepidoptera: Pyralidae)

Cited by 37 publications

References 62 publications

Identification of candidate chemosensory genes by transcriptome analysis in Loxostege sticticalis Linnaeus

Identification of candidate chemosensory genes by transcriptome analysis in Loxostege sticticalis Linnaeus

Transcriptome sequencing reveals abundant olfactory genes in the antennae of the rice leaffolder, Cnaphalocrocis medinalis (Lepidoptera: Pyralidae)

Chemosensory proteins used as target for screening behaviourally active compounds in the rice pest Cnaphalocrocis medinalis (Lepidoptera: Pyralidae)

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