Identification and comparative mapping of a dominant potyvirus resistance gene cluster in Capsicum

Grube, Rebecca C.; Blauth, James R.; Andrés, Mario; Caranta, Carole; Jahn, Molly M.

doi:10.1007/s001220051552

Cited by 71 publications

(32 citation statements)

References 28 publications

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“…The introgression of a new resistance gene will occur at regions of the genome that may already contain resistance genes (Michelmore 2003). So-called ''jackpot'' cultivars can be seen as a source of cassettes of resistances and contain clusters of many tightly linked resistances (Grube et al 2000a). However, merging selected genes of these clusters is a much more daunting prospect.…”

Section: Discussionmentioning

confidence: 99%

The Fractionated Orthology of Bs2 and Rx/Gpa2 Supports Shared Synteny of Disease Resistance in the Solanaceae

et al. 2009

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Comparative genomics provides a powerful tool for the identification of genes that encode traits shared between crop plants and model organisms. Pathogen resistance conferred by plant R genes of the nucleotide-binding-leucine-rich-repeat (NB-LRR) class is one such trait with great agricultural importance that occupies a critical position in understanding fundamental processes of pathogen detection and coevolution. The proposed rapid rearrangement of R genes in genome evolution would make comparative approaches tenuous. Here, we test the hypothesis that orthology is predictive of R-gene genomic location in the Solanaceae using the pepper R gene Bs2. Homologs of Bs2 were compared in terms of sequence and gene and protein architecture. Comparative mapping demonstrated that Bs2 shared macrosynteny with R genes that best fit criteria determined to be its orthologs. Analysis of the genomic sequence encompassing solanaceous R genes revealed the magnitude of transposon insertions and local duplications that resulted in the expansion of the Bs2 intron to 27 kb and the frequently detected duplications of the 59-end of R genes. However, these duplications did not impact protein expression or function in transient assays. Taken together, our results support a conservation of synteny for NB-LRR genes and further show that their distribution in the genome has been consistent with global rearrangements.

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Section: Discussionmentioning

confidence: 99%

The Fractionated Orthology of Bs2 and Rx/Gpa2 Supports Shared Synteny of Disease Resistance in the Solanaceae

et al. 2009

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“…In the first case, monogenic recessive resistance conferred by genes pvr1, pvr2, pvr3, or pvr5 is the result of a passive mechanism in which the absence of interaction between the plant resistance factor and virus virulence factor prevents the virus from completing its replication cycle. On the other hand, the dominant resistance, represented by the presence of genes Pvr4, Pvr6 or Pvr7, is based on the gene-to-gene model (Caranta et al, 1999;Grub et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Inheritance of resistance to Pepper yellow mosaic virus in Capsicum baccatum var. pendulum

Bento¹,

Rodrigues²,

Gonçalves³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. We investigated inheritance of resistance to Pepper yellow mosaic virus (PepYMV) in Capsicum baccatum var. pendulum accessions UENF 1616 (susceptible) crossed with UENF 1732 (resistant). Plants from generations P 1 , P 2 , F 1 , F 2 , BC 1:1 , and BC 1:2 were inoculated and the symptoms were evaluated for 25 days. Subsequently, an area under the disease progress curve was calculated and subjected to generation means analysis. Only the average and epistatic effects were significant. The broad and narrow sense heritability estimates were 35.52 and 21.79%, respectively. The estimate of the minimum number of genes that control resistance was 7, indicating that resistance is polygenic and complex. Thus, methods to produce segregant populations that advocate selection in more advanced generations would be the most appropriate to produce chili pepper cultivars resistant to PepYMV.

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“…A resistência a potyvírus, no pimentão, pode ser controlada por um dos sete locos gênicos da série pvr já identificados, aos quais os autores denominaram: pvr1,pvr2,pvr3,Pvr4,pvr5, pvr6 e Pvr7 (Caranta et al, 1999;Grube et al, 2000b;Parrela et al, 2002). A maioria desses alelos é resistente a duas ou mais espécies de potyvírus.…”

Section: Introductionunclassified

Seleção assistida com uso de marcador molecular para resistência a potyvírus em pimentão

Nogueira

Maluf³

et al. 2012

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi determinar a presença do alelo Pvr4, que confere resistência contra o PepYMV (Pepper yellow mosaic virus), em genótipos de pimentão comunmente encontrados no mercado brasileiro, com uso de um marcador molecular codominante tipo CAPS. A resistência ao PepYMV, nos genótipos CM-334-INRA, Myr-29 e em genótipos derivados do híbrido comercial Mônica-R, foi detectada como associada à banda de 444 pb, ligada ao alelo de resistência Pvr4. As plantas resistentes homozigotas (Pvr4/Pvr4) mostraram uma banda de 444 pb, as suscetíveis (Pvr4 Marker-assisted selection for resistance to potyvirus in sweet pepperAbstract -The objective of this work was to determine the presence of the Pvr4 allele, which controls the resistance to the PepYMV (Pepper yellow mosaic virus), in sweet pepper genotypes commonly available in the Brazilian market, using a CAPS codominant molecular marker. The resistance to PepYMV, in the genotypes CM-334-INRA, Myr-29, and in genotypes derived from the hybrid Mônica-R, was found to be associated with the 444 bp band linked to the resistance allele Pvr4. Homozygous resistant plants (Pvr4/Pvr4) showed a single band of 444 bp, the susceptible ones (Pvr4showed a band of 458 bp, and the heterozygous resistant plants (Pvr4 + /Pvr4) showed both bands. However, in the resistant accession CM-334-UFV, and in the hybrids Magali-R and Martha-R, as well as in populations derived from this accession and these hybrids, the resistance to PepYMV was not associated to the CAPS marker. The accession CM-334-UFV ('Criollo de Morelos-334' from Viçosa, MG, Brazil) was distinct from CM-334-INRA ('Criollo de Morelos-334', from France); although both accessions were resistant to PepYMV, the association of resistance with the 444 bp band was found only in CM-334-INRA.

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Identification and comparative mapping of a dominant potyvirus resistance gene cluster in Capsicum

Cited by 71 publications

References 28 publications

The Fractionated Orthology of Bs2 and Rx/Gpa2 Supports Shared Synteny of Disease Resistance in the Solanaceae

The Fractionated Orthology of Bs2 and Rx/Gpa2 Supports Shared Synteny of Disease Resistance in the Solanaceae

Inheritance of resistance to Pepper yellow mosaic virus in Capsicum baccatum var. pendulum

Seleção assistida com uso de marcador molecular para resistência a potyvírus em pimentão

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