Identification and comparison of aerobic and denitrifying polyphosphate‐accumulating organisms

Zeng, Raymond J.; Saunders, Aaron Marc; Yuan, Zhiguo; Blackall, Linda L.; Keller, Jürg

doi:10.1002/bit.10652

Cited by 161 publications

(90 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This latter phenotype has been demonstrated for enrichment cultures of Accumulibacter spp. (Zeng et al, 2003a) and enrichments of Competibacter spp. (Zeng et al, 2003c).…”

Section: Resultsmentioning

confidence: 99%

Abundance and ecophysiology of Defluviicoccus spp., glycogen-accumulating organisms in full-scale wastewater treatment processes

et al. 2007

Self Cite

View full text Add to dashboard Cite

The activity of glycogen-accumulating organisms (GAOs) in enhanced biological phosphorus removal (EBPR) wastewater treatment plants has been proposed as one cause of deterioration of EBPR. Putative GAOs from the Alphaproteobacteria, Defluviicoccus spp. (including D. vanus), were studied in full-scale EBPR plants to determine their distribution, abundance and ecophysiology. Fluorescence in situ hybridization (FISH) demonstrated that Defluviicoccus spp. were generally low in abundance; however, in one plant surveyed, Cluster 2 Defluviicoccus constituted 9 % of all Bacteria. FISH combined with microautoradiography revealed that both Cluster 1 and Cluster 2 Defluviicoccus were capable of taking up a narrow range of substrates including acetate, propionate, pyruvate and glucose under anaerobic and aerobic conditions. Formate, butyrate, ethanol and several other substrates were not taken up. Cluster 2 Defluviicoccus demonstrated a phenotype consistent with the current metabolic model for GAOs -anaerobic assimilation of acetate and reduction to polyhydroxyalkanoates (PHA) using the glycolytic pathway, and aerobic consumption of PHA. Polyphosphate-accumulating organisms (PAOs, 'Candidatus Accumulibacter phosphatis') and other putative GAOs ('Candidatus Competibacter phosphatis') co-existed in two plants with Cluster 2 Defluviicoccus, but in both plants, the latter organisms were more abundant. Thus Cluster 2 Defluviicoccus can be relatively abundant and could be carbon competitors of PAOs and other GAOs in EBPR plants.

show abstract

“…This latter phenotype has been demonstrated for enrichment cultures of Accumulibacter spp. (Zeng et al, 2003a) and enrichments of Competibacter spp. (Zeng et al, 2003c).…”

Section: Resultsmentioning

confidence: 99%

Abundance and ecophysiology of Defluviicoccus spp., glycogen-accumulating organisms in full-scale wastewater treatment processes

et al. 2007

Self Cite

View full text Add to dashboard Cite

show abstract

“…Their taxonomy is now well understood with Garcia Martin et al (2006) obtaining a complete genome of Accumulibacter, which has provided insights into gene expression involved in key metabolic processes. Furthermore, Accumulibacter are considered the prevalent PAO in effective in EPBR systems (Zeng et al, 2003;López-Vázquez et al, 2008), although other PAO are present too.…”

Section: Identification Of Paomentioning

confidence: 99%

A Review of Phosphorus Removal Technologies and Their Applicability to Small-Scale Domestic Wastewater Treatment Systems

Bunce

Ndam

Ofiţeru

et al. 2018

Front. Environ. Sci.

406

204

View full text Add to dashboard Cite

The removal of phosphorus (P) from domestic wastewater is primarily to reduce the potential for eutrophication in receiving waters, and is mandated and common in many countries. However, most P-removal technologies have been developed for use at larger wastewater treatment plants that have economies-of-scale, rigorous monitoring, and in-house operating expertise. Smaller treatment plants often do not have these luxuries, which is problematic because there is concern that P releases from small treatment systems may have greater environmental impact than previously believed. Here P-removal technologies are reviewed with the goal of determining which treatment options are amenable to small-scale applications. Significant progress has been made in developing some technologies for small-scale application, namely sorptive media. However, as this review shows, there is a shortage of treatment technologies for P-removal at smaller scales, particularly sustainable and reliable options that demand minimal operating and maintenance expertise or are suited to northern latitudes. In view of emerging regulatory pressure, investment should be made in developing new or adapting existing P-removal technologies, specifically for implementation at small-scale treatment works.

show abstract

“…Fluorescence in situ hybridization (FISH) has shown Accumulibacter to be an abundant organism in laboratory-scale EBPR cultures with various carbon sources (Levantesi et al, 2002;Liu et al, 2001;Oehmen et al, 2004Oehmen et al, , 2005bOnda et al, 2002;Pijuan et al, 2004;Zeng et al, 2003a). Accumulibacter has also been reported in full-scale EBPR systems (Saunders et al, 2003;Zilles et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Anaerobic and aerobic metabolism of glycogen-accumulating organisms selected with propionate as the sole carbon source

et al. 2006

Self Cite

View full text Add to dashboard Cite

In the microbial competition observed in enhanced biological phosphorus removal (EBPR) systems, an undesirable group of micro-organisms known as glycogen-accumulating organisms (GAOs) compete for carbon in the anaerobic period with the desired polyphosphate-accumulating organisms (PAOs). Some studies have suggested that a propionate carbon source provides PAOs with a competitive advantage over GAOs in EBPR systems; however, the metabolism of GAOs with this carbon source has not been previously investigated. In this study, GAOs were enriched in a laboratory-scale bioreactor with propionate as the sole carbon source, in an effort to better understand their biochemical processes. Based on comprehensive solid-, liquid-and gas-phase chemical analytical data from the bioreactor, a metabolic model was proposed for the metabolism of propionate by GAOs. The model adequately described the anaerobic stoichiometry observed through chemical analysis, and can be a valuable tool for further investigation of the competition between PAOs and GAOs, and for the optimization of the EBPR process. A group of Alphaproteobacteria dominated the biomass (96 % of Bacteria) from this bioreactor, while postfluorescence in situ hybridization (FISH) chemical staining confirmed that these Alphaproteobacteria produced poly-b-hydroxyalkanoates (PHAs) anaerobically and utilized them aerobically, demonstrating that they were putative GAOs. Some of the Alphaproteobacteria were related to Defluvicoccus vanus (16 % of Bacteria), but the specific identity of many could not be determined by FISH. Further investigation into the identity of other GAOs is necessary.

show abstract

Identification and comparison of aerobic and denitrifying polyphosphate‐accumulating organisms

Cited by 161 publications

References 28 publications

Abundance and ecophysiology of Defluviicoccus spp., glycogen-accumulating organisms in full-scale wastewater treatment processes

Abundance and ecophysiology of Defluviicoccus spp., glycogen-accumulating organisms in full-scale wastewater treatment processes

A Review of Phosphorus Removal Technologies and Their Applicability to Small-Scale Domestic Wastewater Treatment Systems

Anaerobic and aerobic metabolism of glycogen-accumulating organisms selected with propionate as the sole carbon source

Contact Info

Product

Resources

About