Identification and Comparison of Candidate Olfactory Genes in the Olfactory and Non-Olfactory Organs of Elm Pest Ambrostoma quadriimpressum (Coleoptera: Chrysomelidae) Based on Transcriptome Analysis

Wang, Yinliang; Chen, Qi; Zhao, Hanbo; Ren, Bingzhong

doi:10.1371/journal.pone.0147144

Cited by 32 publications

(34 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, the antennae, consistent with previous observations, were markedly longer in males than females. The longer male antennae confer an adaptive significance as they provide more surface area for sense organs to help detect sex pheromones efficiently (Schneider, 1964;Dweck 2009;Wang et al, 2016). In the present study, the density of sensilla, especially sensilla trichodea, was found to be greater in terminal segments (Figure 2).…”

Section: Types Of Sensillasupporting

confidence: 50%

Sexual dimorphism in antennal sensilla of Parthenium beetle Zygogramma bicolorata

Qadir¹,

Qamar²

2019

Turk J Zool

View full text Add to dashboard Cite

In the present study, scanning electron microscopy was used to investigate the ultrastructure of sensilla present on the antennae of male and female Zygogramma bicolorata. The antennae in this insect consist of the scape, pedicel, and nine flagellomeres. Six types of sensilla were identified: sensilla trichodea type 1 and 2 (ST1, ST2), sensilla cavitae (SCa), sensilla chaetica (SCh), and sensilla basiconica type 1 and 2 (SB1, SB2). Sensilla were characterized on the basis of their size and density to find out whether sexual dimorphism exists in this species or not. We observed that the size of the antennomeres was comparatively larger in males than females. Moreover, sensilla chaetica were found on all antennomers and have the qualification of being the longest type, suggesting that they probably have a role in mechanoreception. There was an abundance of sensilla trichodea in both males and females, indicating its function as a chemoreceptor. Sensilla basiconica are believed to sense sex pheromones and hence their abundance was more in males as compared to females.

show abstract

Section: Types Of Sensillasupporting

confidence: 50%

Sexual dimorphism in antennal sensilla of Parthenium beetle Zygogramma bicolorata

Qadir¹,

Qamar²

2019

Turk J Zool

View full text Add to dashboard Cite

show abstract

“…data), so its poor support in the present study is questionable, but it may reflect a division that will become evident as additional beetle genomes are sequenced. The inclusion of TcasOR71-72 in Group 7 is also probably in error, because their placement is inconsistent in studies to date (Engsontia et al 2008; Wang et al 2016; RFM unpub. data).…”

Section: Discussionmentioning

confidence: 99%

“…ORs of A. glabripennis were separately aligned with the three functionally characterized ORs from M. caryae , the OR genes available from the genome of T. castaneum , and published ORs from transcriptomes of three other major beetle families: Chrysomelidae ( Ambrostoma quadriimpressum Motchulsky; Wang et al 2016), Scarabaeidae ( Anomala corpulenta Motchulsky; Li et al 2015), and Curculionidae ( D. ponderosae ). Only genes longer than 100 amino acids were included in this analysis.…”

Section: Methodsmentioning

confidence: 99%

Odorant receptors and antennal lobe morphology offer a new approach to understanding olfaction in the Asian longhorned beetle

Mitchell

Hall

Reagel³

et al. 2017

J Comp Physiol A

View full text Add to dashboard Cite

The Asian longhorned beetle Anoplophora glabripennis (Motchulsky) is an exotic forest pest that has repeatedly invaded North America and Europe from Asia, and has the potential to kill millions of trees and cause billions of dollars in damage. Traps baited with an attractive mixture of volatile organic compounds (VOCs) from hosts have been of limited success in monitoring invasion sites. We propose that lures might be improved through studying the olfactory system of adult beetles, especially the gene family of odorant receptors (ORs) and the structure of the antennal lobes of the brain. Here, we report identification of 132 ORs in the genome of A. glabripennis (inclusive of one Orco gene and 11 pseudogenes), some of which are orthologous to known pheromone receptors of other cerambycid beetles. We also identified three ORs that are strongly biased toward expression in the female transcriptome, and a single OR strongly biased toward males. Three-dimensional reconstruction of the antennal lobes of adults suggested a male-specific macroglomerulus and several enlarged glomeruli in females. We predict that functional characterization of ORs and glomeruli will lead to identification of key odorants in the life history of A. glabripennis that may aid in monitoring and controlling future invasions.

show abstract

“…The primers used for qPCR are listed in Supplementary Table S4, and GAPDH was used as an internal reference control. The qPCR conditions used were as described by Wang et al (2016), and the relative expression levels were calculated using the 2 − Ct method (Pfaffl, 2001). Statistical analysis was conducted by GraphPad Prism software (San Diego, CA, United States) (Wang et al, 2016).…”

Section: Verification Of Tmt Data On Selected Candidates By Qpcrmentioning

confidence: 99%

TMT-Based Quantitative Proteomic Profiling of Overwintering Lissorhoptrus oryzophilus

et al. 2020

View full text Add to dashboard Cite

Adaptations to low temperature play a critical role in restricting the geographical distribution of insects. Decreasing day lengths and temperatures trigger seasonal cold adaptations in insects. These adaptions include changes in expression at the miRNA, mRNA and protein levels. The rice water weevil (RWW), Lissorhoptrus oryzophilus, introduced from the Mississippi River, is a globally invasive pest of wetland rice that can survive at the northern border of China. To investigate the changes in expression at the protein level in overwintering female RWW adults, 6-plex tandem mass tags (TMTs) were used in overwintering and summer adults. By using a proteome database available for Curculionidae, 1077 proteins were quantified, 183 of which differed significantly between the overwintering and summer samples. To further understand these differentially expressed proteins (DEPs), bioinformatics analyses such as gene ontology (GO) enrichment analyses were performed. DEPs associated with the terms binding, structural molecule activity, catalytic activity, multicellular organismal process, extracellular region, chitin binding, metabolic process, intracellular part and organic cyclic compound binding were altered by selection during winter. The changes in the expression of these proteins suggest that the proteins are important for RWW survival in winter.

show abstract

Identification and Comparison of Candidate Olfactory Genes in the Olfactory and Non-Olfactory Organs of Elm Pest Ambrostoma quadriimpressum (Coleoptera: Chrysomelidae) Based on Transcriptome Analysis

Cited by 32 publications

References 40 publications

Sexual dimorphism in antennal sensilla of Parthenium beetle <i>Zygogramma</i> <i>bicolorata</i>

Sexual dimorphism in antennal sensilla of Parthenium beetle <i>Zygogramma</i> <i>bicolorata</i>

Odorant receptors and antennal lobe morphology offer a new approach to understanding olfaction in the Asian longhorned beetle

TMT-Based Quantitative Proteomic Profiling of Overwintering Lissorhoptrus oryzophilus

Contact Info

Product

Resources

About