Identification and Comparison of Constituents of Aurantii Fructus and Aurantii Fructus Immaturus by UFLC-DAD-Triple TOF-MS/MS

Abstract

Although Aurantii Fructus (AF) and Aurantii Fructus Immaturus (AFI) are both the fruits of the same rutaceae plant at different stages of growth, they exert similar yet distinct clinical effects. The chemical composition is crucial for quality control as well as therapeutic application. To address this concern, it is significant to evaluate the similarities and differences of the constituents in both AF and AFI. The extract of AF and AFI were comprehensively analyzed by ultra fast liquid chromatography-photodiode array detector-triple-time of flight-tandem mass spectrometry (UFLC-DAD-Triple TOF-MS/MS). Among the 40 compounds detected, 19 metabolites were detected in both the AF and AFI; whereas 13 compounds were only detected in AF and five constituents were exclusively detected in AFI. In particular, even in AFI, three compounds were only identified in AFI (Citrus aurantium’ L. and its cultivar). Among the 18 compounds confirmed by standard database, 13 compounds were reported in AF and AFI for the first time. Furthermore, the distinction was also revealed by the content of naringin, hesperidin, neohesperidin, and synephrine. The study directly contributed to the similarities and differences of AF and AFI. Herein, similarities and the differences in chemical profiles of AF and AFI could explain the current clinical applications.

Isolation and Identification of Chemical Constituents from Zhideke Granules by Ultra-Performance Liquid Chromatography Coupled with Mass Spectrometry

“…Subsequently, the ion at m / z 327.0849 [M + H-2CH 3 -H 2 O-CO] + originated from the ion at m / z 373.0900 by loss of H 2 O (18 Da) and CO (28 Da). Compound 18 was characterized as nobiletin [ 17 ] according to these results.…”

Characterisation and classification of Citri Reticulatae Pericarpium varieties based on UHPLC‐Q‐TOF‐MS/MS combined with multivariate statistical analyses

“…Compared with CP, GCP processed relative higher content of rhoifolin, vanillic acid, and diosmetin‐6‐ C ‐glucoside, and a lower content of rutin, citrusin I, luteolin‐6,8‐di‐ C ‐glucoside, and isoorientin. The chemical diversity may be caused by many factors, such as their genetic origin, growing environment and time of collection, peculiar to the genetic origin …”

Rapid Profiling of Chemical Constituents in Qingfei Paidu Granules Using High Performance Liquid Chromatography Coupled with Q Exactive Mass Spectrometry

“…The mass spectrum of compound 83 exhibited peaks representing [M + H] + at m/z 471.2016 and fragment ions at m/z 425.1957 [M + H − 46] + and 161.0597. According to the literature [ 23 ] and the data in the OTCML, it was limonin.…”