Identification and complete genomic sequence of a novel sadwavirus discovered in chrysanthemum (Chrysanthemum morifolium Ramat)

Chen, Jie; Dong, Yafeng; Wang, Hui; Zhang, jie; Changnian, Ma; Cao, Liangliang; Shen, Leiding; Cao, Kuirong; Fan, Xudong

doi:10.21203/rs.3.rs-3247284/v1

Cited by 2 publications

(1 citation statement)

References 7 publications

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“…Interestingly, the sizes of polyproteins and predicted polyprotein cleavage sites of ChrSV, a stramovirus identi ed in Chrysanthemum morifolium transcriptome in this study were similar to those of chrysanthemum sadwavirus (ChSV) identi ed in diseased C. morifolium plants (Chen et al, 2023). Similar pattern of phylogenetic clustering was observed for ChrSV identi ed in this study and ChSV identi ed by Chen et al (2023).…”

Section: Discussionsupporting

confidence: 81%

Probing of plant transcriptomes unveils the hitherto hidden genetic diversity of the family Secoviridae

Sidharthan,

Reddy,

Kiran

et al. 2023

Preprint

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Secoviridae family contains single stranded RNA genome-containing viruses that infect plants. In the present study, we mined publicly available plant transcriptomes and identified sixty-one putative novel secoviral sequences in various plant species ranging from bryophytes to trees, which increased the known secoviral diversity by approximately 0.5-fold. Of the identified viral sequences, 13 were monopartite and 48 were bipartite, and sequences of 52 secoviruses were coding-complete and nine were partial. Except for small open reading frames (ORFs) determined in waikaviral genomes and RNA2 of torradoviruses, all the recovered genomes/genome segments contained a large ORF encoding a polyprotein. Based on genome organization, sequence similarity to known members, phylogeny and secovirus species demarcation criteria, all but three identified novel secoviruses were assigned to different secoviral genera- Cheravirus (3), Comovirus (2), Fabavirus (5), Nepovirus (29), Sadwavirus (3), Sequivirus (1), Stralarivirus (1), Torradovirus (4) and Waikavirus (10). Genome organization of two of the identified waika-like viruses resembled that of the recently identified waika-like virus- Triticum aestivum secovirus. Phylogenetic analysis revealed the host-waikavirus co-evolution pattern in a few waika- and waika-like viruses, the increased phylogenetic diversity of nepoviruses and the phylogenetic clustering of waika-like viruses. The study paves way for further studies on understanding the biological properties of identified novel secoviruses.

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Section: Discussionsupporting

confidence: 81%