Identification and control potential of entomopathogenic nematodes against the black cutworm, Agrotis ipsilon (Fabricius) (Lepidoptera: Noctuidae), in potato-growing areas of Turkey

Askar, Arife Gümüş; Yüksel, Ebubekir; Öcal, Atilla; Özer, Göksel; Kütük, Halil; Dababat, Abdelfattah A.; İmren, Mustafa

doi:10.1007/s41348-022-00566-y

Cited by 4 publications

(6 citation statements)

References 47 publications

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“…Four new strains of S. feltiae were isolated, being the most common species detected globally (57%) and in the region of Teruel (100%). In fact, S. feltiae is frequently reported as the dominant EPN species in Mediterranean countries, accounting for 38% in Italy (Tarasco et al 2015), 55% (Campos-Herrera et al 2007) and 70% in Spain (Garcia del Pino and Palomo, 1996), 75% in Portugal (Valadas et al 2013), 71–85% in Turkey (Hazir et al 2003; Yuksel and Canhilal 2019; Gümüş Askar et al 2022) and 87% in Algeria (Tarasco et al 2009). In the region of Catalonia, three strains of H. bacteriophora were identified and were the most common species detected (75%).…”

Section: Discussionmentioning

confidence: 99%

Natural occurrence of entomopathogenic nematodes (Steinernema and Heterorhabditis) and Pristionchus nematodes in black truffle soils from Spain

Julià,

Morton,

Garcia-del-Pino

2023

J. Helminthol.

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The European truffle beetle Leiodes cinnamomeus is the most important pest in black truffle (Tuber melanosporum) plantations. Current control methods against it are inefficient, so entomopathogenic nematodes (EPNs) could play an important role in their population regulation due to their efficacy against many soil-dwelling insect pests. A survey of EPNs and Pristionchus nematodes was conducted in truffle soils of Spain, considering environmental and physical–chemical soil factors. A total of 164 soil samples were collected from forests, productive plantations and null-low productive plantations, representing three distinct black truffle-growing habitat types. EPNs were isolated from seven soil samples (4.3%); four nematodes were identified as Steinernema feltiae and three as Heterorhabditis bacteriophora. Both species were sampled in three types of soil texture (loam, sandy loam or sandy clay loam), characterized by alkaline pH (7.5 to 8.5) and high organic matter (2.1–11.04%). The presence of these EPNs was influenced by habitat type and organic matter content. Pristionchus nematodes were isolated from truffle soil, around truffle fruit bodies and under the elytra of L. cinnamomeus, with Pristionchus maupasi being the most commonly identified species. No significant associations were found between environmental and soil factors and the occurrence of Pristionchus nematodes. These nematodes were found in alkaline soils (pH 7.75 to 8.7), across all seven sampled soil textures, with variable organic matter content (0.73%–5.92%). The ecological trends and the presence of Pristionchus may affect the occurrence of EPNs and their prospective use as biological control agents against L. cinnamomeus in black truffle plantations.

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Section: Discussionmentioning

confidence: 99%

Natural occurrence of entomopathogenic nematodes (Steinernema and Heterorhabditis) and Pristionchus nematodes in black truffle soils from Spain

Julià,

Morton,

Garcia-del-Pino

2023

J. Helminthol.

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“…Therefore, preliminary pathogenicity screening tests provide valuable information before evaluation of performance of EPNs [ 46 ]. In the present study, the most pathogenic EPN species that proved to be highly virulent on the larvae of G. mellonella in an earlier study were used [ 30 ]. In the aforementioned studies, different wireworm and EPN species were utilized in the bioassays which might be one possible reason leading to variations in the mortality rates.…”

Section: Discussionmentioning

confidence: 99%

“…Three EPN species that were previously recovered from potato fields were employed in in vitro bioassays [ 30 ] ( Table 1 ). In order to obtain a fresh batch of IJs, EPN species were multiplied in vitro on the last instar larvae of Galleria mellonella (L.) (Lepidoptera: Pyralidae) [ 25 ].…”

Section: Methodsmentioning

confidence: 99%

“…In general, local EPNs are considered well-adapted to climatic and environmental conditions where they are isolated and can effectively suppress the pest populations without adverse effects on non-target species [ 26 , 27 , 28 , 29 ]. Therefore, in a previous study, a wide-ranging field survey was conducted in major potato cultivation areas of Türkiye to identify EPN species for the control of major potato pests [ 30 ]. In the present study, the effectiveness of isolated EPN species/strains was evaluated against the larvae of A. sputator and A. rufipalpis which are the two predominant wireworm species in potato growing areas of Türkiye [ 31 ].…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of Entomopathogenic Nematodes against Common Wireworm Species in Potato Cultivation

et al. 2023

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Wireworms (Coleoptera: Elateridae) are common insect pests that attack a wide range of economically important crops including potatoes. The control of wireworms is of prime importance in potato production due to the potential damage of the larvae to tuber quantity and quality. Chemical insecticides, the main control strategy against wireworms, generally fail to provide satisfactory control due to the lack of available chemicals and the soil-dwelling habits of the larvae. In the last decades, new eco-friendly concepts have emerged in the sustainable control of wireworms, one of which is entomopathogenic nematodes (EPNs). EPNs are soil-inhabitant organisms and represent an ecological approach to controlling a great variety of soil-dwelling insect pests. In this study, the susceptibility of Agriotes sputator Linnaeus and A. rufipalpis Brullé larvae, the most common wireworm species in potato cultivation in Türkiye, to native EPN strains [Steinernema carpocapsae (Sc_BL22), S. feltiae (Sf_BL24 and Sf_KAY4), and Heterorhabditis bacteriophora (Hb_KAY10 and Hb_AF12)] were evaluated at two temperatures (25 and 30 °C) in pot experiments. Heterorhabditis bacteriophora Hb_AF12 was the most effective strain at 30 °C six days post-inoculation and caused 37.5% mortality to A. rufipalpis larvae. Agriotes sputator larvae were more susceptible to tested EPNs at the same exposure time, and 50% mortality was achieved by two EPNs species, Hb_AF12 and Sc_BL22. All EPN species/strains induced mortality over 70% to both wireworm species at both temperatures at 100 IJs/cm2, 18 days post-treatment. The results suggest that tested EPN species/strains have great potential in the control of A. sputator and A. rufipalpis larvae.

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“…Steinernema feltiae and H. bacteriophora are the most frequently detected EPN species in Türkiye and other parts of the world, respectively [57][58][59][60]. However, some studies reported a higher occurrence of H. bacteriophora than S. feltiae from neighboring regions with similar climatic and geographic conditions in Türkiye [61,62]. Although both EPN species have been found in different climatic zones, S. feltiae was more dominant in cold and continental climate conditions, whereas H. bacteriophora was generally isolated in higher frequencies from tropical and subtropical areas [51,63,64].…”

Section: Discussionmentioning

confidence: 99%

Identification and Biocontrol Potential of Entomopathogenic Nematodes and Their Endosymbiotic Bacteria in Apple Orchards against the Codling Moth, Cydia pomonella (L.) (Lepidoptera: Tortricidae)

Gümüşsoy

Yüksel

Özer

et al. 2022

Insects

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The codling moth, Cydia pomonella (L.) (Lepidoptera: Tortricidae), is one of the major pests in pome fruit production worldwide. Heavy treatment of the larvae of C. pomonella with insecticides triggered the development of resistance to many groups of insecticides. In addition, the increasing concern about the adverse effects of synthetic insecticides on human health and the environment has led to the development of sustainable and eco-friendly control practices for C. pomonella. The entomopathogenic nematodes (EPNs) (Steinernema and Heterorhabditis spp.) and their endosymbionts (Xenorhabdus and Photorhabdus spp.) represent a newly emerging approach to controlling a wide range of insect pests. In the present study, field surveys were conducted in apple orchards to isolate and identify EPNs and their endosymbionts and evaluate their insecticidal efficacy on the larvae of C. pomonella. EPNs were isolated from 12 of 100 soil samples (12%). Seven samples were identified as Steinernema feltiae (Filipjev, 1934) (Rhabditida: Steinernematidae), whereas five samples were assigned to Heterorhabditis bacteriophora (Poinar, 1976) (Rhabditida: Heterorhabditidae). The pathogenicity of the EPN species/isolates was screened on the last instar larvae of G. mellonella. The two most pathogenic isolates from each EPN species were tested against fifth instar larvae of C. pomonella under controlled conditions. The maximum mortality (100%) was achieved by all EPN species/isolates at a concentration of 100 IJs/larva 96 h after treatment. The endosymbionts of selected H. bacteriophora and S. feltiae species were identified as Photorhabdus luminescens subsp. kayaii and Xenorhabdus bovienii, respectively. The mortality rates ranged between 25 and 62% when the fifth larval instar larvae of C. pomonella were exposed to the treatment of cell-free supernatants of symbiotic bacteria. In essence, the present survey indicated that EPNs and their symbiotic bacteria have good potential for biological control of C. pomonella.

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Identification and control potential of entomopathogenic nematodes against the black cutworm, Agrotis ipsilon (Fabricius) (Lepidoptera: Noctuidae), in potato-growing areas of Turkey

Cited by 4 publications

References 47 publications

Natural occurrence of entomopathogenic nematodes (Steinernema and Heterorhabditis) and Pristionchus nematodes in black truffle soils from Spain

Natural occurrence of entomopathogenic nematodes (Steinernema and Heterorhabditis) and Pristionchus nematodes in black truffle soils from Spain

Evaluation of Entomopathogenic Nematodes against Common Wireworm Species in Potato Cultivation

Identification and Biocontrol Potential of Entomopathogenic Nematodes and Their Endosymbiotic Bacteria in Apple Orchards against the Codling Moth, Cydia pomonella (L.) (Lepidoptera: Tortricidae)

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