Identification and Defensive Characterization of PmCYP720B11v2 from Pinus massoniana

Abstract

Pinus massoniana is a pioneer species for afforestation timber and oleoresin, while epidemics of pinewood nematode (PWN; Bursaphelenchus xylophilus) are causing a serious biotic disaster for P. massoniana in China. Importantly, resistant P. massoniana could leak copious oleoresin terpenoids to build particular defense fronts for survival when attacked by PWN. However, the defense mechanisms regulating this process remain unknown. Here, PmCYP720B11v2, a cytochrome P450 monooxygenase gene, was first identified and functionally characterized from resistant P. massoniana following PWN inoculation. The tissue-specific expression pattern and localization of PmCYP720B11v2 at the transcript and protein levels in resistant P. massoniana indicated that its upregulation in the stem supported its involvement in the metabolic processes of diterpene biosynthesis as a positive part of the defense against PWN attack. Furthermore, overexpression of PmCYP720B11v2 may enhance the growth and development of plants. In addition, PmCYP720B11v2 activated the metabolic flux of antioxidases and stress-responsive proteins under drought conditions and improved drought stress tolerance. Our results provide new insights into the favorable role of PmCYP720B11v2 in diterpene defense mechanisms in response to PWN attack in resistant P. massoniana and provide a novel metabolic engineering scenario to reform the stress tolerance potential of tobacco.

Identification of Genes and Metabolic Pathways Involved in Resin Yield in Masson Pine by Integrative Analysis of Transcriptome, Proteome and Biochemical Characteristics

“…Of these known spruce and pine CYP720Bs, four have been proven to be involved in the biosynthesis of DRAs, two in clade I (CYP720B2, CYP720B12) and two in clade III (CYP720B1, CYP720B4) [ 49 , 50 , 51 ]. In a recent study, the PmCYP720B11v2 gene was also proven to be involved in the metabolic processes of diterpene biosynthesis in the stem as a positive part of the defense against pinewood nematode (PWN) attack in masson pine [ 52 ]. In the present study, three genes in clade I ( CYP720B2 , CYP720B10 , CYP720B11 ), one gene in clade III ( CYP720B1 ), and one gene in clade IV ( CYP720B8 ) were identified in needles.…”

Plant peptide hormone phytosulfokine promotes embryogenic development of calli in Pinus massoniana

“…P. massoniana is a fast-growing afforestation tree species that is native to China, accounting for 8.09% of the country's forests, the species supports the development of numerous industries, such as wood, paper making, and forestry chemicals (Liu et al 2022). Studies on P. massoniana SE began in 1995, four regenerated plants were obtained successfully with mature P. massoniana zygotic embryos as explants (Huang et al 1995).…”

A Bursaphelenchus xylophilus effector BxICD1 inducing plant cell death, concurrently contributes to nematode virulence and migration

“…Masson pine ( Pinus massoniana ) is a pioneer species for afforestation timber and oleoresin production, widely distributed in South China. However, it is highly sensitive to B. xylophilus ( Xue et al., 2013 ; Liu et al., 2022a ). Despite this, the roles of effectors during B. xylophilus parasitism in P. massoniana are poorly understood.…”