Identification and determination of characteristics of endophytes from rice plants and their role in biocontrol of bacterial blight caused by Xanthomonas oryzae pv. oryzae

Yousefi, Hadis; Hassanzadeh, N.; Behboudi, Keivan; Firouzjahi, Farid Beiki

doi:10.2478/hppj-2018-0003

Cited by 10 publications

(4 citation statements)

References 62 publications

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“…Plant roots, leaves and stems were rst washed in running tap water to remove any soil or contaminant from the eld before being washed in 70% ethanol for 5 minutes. They were transferred to 3% sodium hypochlorite for ve minutes for complete surface sterilization and then rinsed several times in sterile distilled water [27]. The e ciency of surface sterilization was assessed by inoculating 100 µL aliquot of the last rinsing water on Nutrient agar plates and incubating for 48 hours at 28 ±2 ○ C. Absence of any growth indicated complete surface sterilization.…”

Section: Isolation Of Culturable Bacterial Endophytesmentioning

confidence: 99%

Diversity of Culture dependent endophytic bacteria isolated from leguminous agroforestry trees in western Kenya

Emitaro

Kawaka

Musyimi

et al. 2022

Preprint

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Plants have diverse and vast niches colonized by endophytic microorganisms that promote the well being of host plant. These microbes inhabit internal plant tissues with no signs of ill health. Bacterial endophytes from many plants have been isolated and characterized due to their beneficial roles however their diversity in leguminous plants still remain unexploited. Diversity of bacterial endophytes isolated from L. diversifolia, S. sesban and C. Salothyrsus were assessed using morphological and molecular characteristics. A total of 27 pure isolates were recovered from C. Calothyrsus, L. diversifolia and S. sesban constituting 44.4%, 31.8% and 23.8% from the leaves, stems and roots respectively. The isolates differentiated into Gram positive and negative with rods and spherical shapes. Analysis of 16S rRNA gene sequence revealed 10 closely related bacterial genera that consisted of Bacilli (33.3%), Staphylococcus (22.2%), Alcaligens (11.2%), Xanthomonas and Sphingomonas (47.4 %). Others included Enterobacteria, Pantoea, Acinetobacter, Pseudomonas and Bacterium. These results indicate the presence of high diversity of endophytic bacteria associated with the different parts of L. diversifolia, S. sesban and C. salothyrsus growing in western Kenya.

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Section: Isolation Of Culturable Bacterial Endophytesmentioning

confidence: 99%

Diversity of Culture dependent endophytic bacteria isolated from leguminous agroforestry trees in western Kenya

Emitaro

Kawaka

Musyimi

et al. 2022

Preprint

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“…Seed treatment with these antagonists caused disease to cease in over 60% of plants. These species have been found as endophytes in rice (Yousefi et al 2018). Endophytic bacteria are able to control the disease caused by bacteria and fungi because they produce hydrolytic enzyme as well as chitinase, protease similar to rhizospheric bacteria B. subtilis from potato rhizosphere (Lestari et al 2017).…”

Section: Suppressing Bacterial Leaf Blight By Ebarrmentioning

confidence: 99%

Endophytic bacteria associated with rice roots from suboptimal land as plant growth promoters

2021

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Abstract. Prihatiningsih N, Djatmiko HA, Lestari P. 2021. Endophytic bacteria associated with rice roots from suboptimal land as plant growth promoters. Biodiversitas 22: 432-437. Endophytic bacteria directly promoted plant growth and undirectly control the pathogens. This research was aimed to evaluate endophytic bacteria associated with rice root and their activity to promote plant growth and to control rice diseases. The study was conducted at the Laboratorium of Plant Protection and experimental farm Faculty of Agriculture Jenderal Soedirman University, from April to August 2020. The endophytic bacterial from suboptimal land were evaluated for promoting plant growth with soaking seed before seedling and spraying them at 10, 20, and 30 days after transplanting. The experiment was arranged with six replications and four treatments namely control (untreated endophytic bacteria) SM1 (endophytic bacteria isolate from Somagede); SB1 and SB3 (from Sumbang). Xanthomonas oyzae pv. oryzae was nature inoculation because the experiment location is in the endemic category of bacterial leaf blight. The variables observed were the plant growth components i.e plant height, number of tillers, number of panicles, incubation period, disease intensity, infection rate and effectiveness control. The result of this research shows that endophytic bacteria from Somagede (SM1) is the best to enhance plant height and number of tillers, and suppress disease intensity, and delay of incubation period.

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“…They occupy the same niche inside host plants as that of pathogens and hence can be possibly utilized as good candidates for the management of plant pathogens. Yousefi et al (2018) reported the antagonistic potential of endophytes isolated from rice against BLB. Four endophytes 37.25, 34.25, 33.25 and 28.5% reduction of BLB incidence over control.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of rice associated Bacillus spp. against sheath blight and bacterial blight of rice

Surendran¹,

Thomas²,

Jose³

et al. 2022

JBC

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Bacterial antagonist, Bacillus spp. cultures were isolated from different places of Kuttanad region. Three effective endophytic Bacillus strains viz., B 15, B 17 and B 33 were tested in the separate field experiments against sheath blight and bacterial blight diseases of rice during Kharif 2017, Rabi 2018-19, Kharif 2019 and Kharif 2020. In the field study, the Bacillus cultures were treated as standard bioagent application methods of seed (10 g/kg), soil (1 kg/acre) and foliar (20 g/litre of water) spraying against the major diseases in rice. The rice associated native Bacillus cultures B 15, B 17 and B 33 were found equally effective for the sheath blight and bacterial blight diseases management. All the three native Bacillus species can be used as potential biopesticides against rice sheath blight and bacterial diseases in Kuttanad region.

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Identification and determination of characteristics of endophytes from rice plants and their role in biocontrol of bacterial blight caused by Xanthomonas oryzae pv. oryzae

Cited by 10 publications

References 62 publications

Diversity of Culture dependent endophytic bacteria isolated from leguminous agroforestry trees in western Kenya

Diversity of Culture dependent endophytic bacteria isolated from leguminous agroforestry trees in western Kenya

Endophytic bacteria associated with rice roots from suboptimal land as plant growth promoters

Evaluation of rice associated <i>Bacillus</i> spp. against sheath blight and bacterial blight of rice

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