Identification and determination of nucleosides in Cordyceps sinensis and its substitutes by high performance liquid chromatography with mass spectrometric detection

Guo, Fang-Qiu; Ai, Li; Huang, Lan-Fang; Liang, Yi‐Zeng; Chen, Ben-Mei

doi:10.1016/j.jpba.2005.07.034

Cited by 58 publications

(44 citation statements)

References 14 publications

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“…The detection limits were 12.6 ng/mL (adenosine), 16.0 ng/mL (cordycepin), 15.1 ng/mL (2′-deoxyadenosine), 9.5 ng/mL (guanosine), 6.5 ng/mL (hypoxanthine) and 9.7 ng/mL (uridine) at a signal-to-noise ratio of 3. The sensitivity of the proposed method for nucleic acid-related compounds was higher than that of CE-UV detection (Li et al, 2002;Gong et al, 2004) and LC-MS detection (Guo et al, 2006;Huang et al, 2003). However, HPLC-MS/MS detection (Fan et al, 2006) was approximately 10-fold more sensitive than the present method.…”

Section: Methods Validationmentioning

confidence: 61%

“…Therefore, it is necessary to determine further natural CS samples to certificate the contents of nucleic acid-related compounds. Recently, cordycepin in natural CS was found at a very low content by LC-MS (Guo et al, 2006) and HPLC-UV (Li et al, 2002) methods. However, the sensitivity of the latter method was not enough to determine such low contents of cordycepin.…”

Section: Contents Of Nucleosides In Cordycepsmentioning

confidence: 99%

“…Several reports have indicated nucleosides, especially adenosine, to be important markers of Cordyceps, and a number of methods have been developed for their determination, including thin-layer chromatography using stepwise gradient elution (Ma et al, 2004), liquid chromatography (LC) with mass spectrometric (MS) detection (Guo et al, 2006;Huang et al, 2003;Fan et al, 2006) or UV detection and capillary electrophoresis (CE) with UV detection (Li et al, 2002;Gong et al, 2004). Sample pretreatment methods such as solid-phase extraction (Ma et al, 2004), pressurized liquid extraction (Fan et al, 2006;Li et al, 2004), soxhlet extraction and sonication Guo et al, 2006;Huang et al, 2003) have also been developed using different kinds of extraction solvent including methanol, water and borate buffer.…”

Section: Introductionmentioning

confidence: 99%

“…Sample pretreatment methods such as solid-phase extraction (Ma et al, 2004), pressurized liquid extraction (Fan et al, 2006;Li et al, 2004), soxhlet extraction and sonication Guo et al, 2006;Huang et al, 2003) have also been developed using different kinds of extraction solvent including methanol, water and borate buffer.…”

Section: Introductionmentioning

confidence: 99%

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Simple HPLC‐UV determination of nucleosides and its application to the authentication of Cordyceps and its allies

Ikeda

Nishimura

Sun

et al. 2008

Biomedical Chromatography

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A simple HPLC-UV method combined with a simple extraction procedure of nucleosides (adenosine, cordycepin, 2'-deoxyadenosine, guanosine and uridine) was developed and applied to the authentication of Cordyceps and its allies. The separation was performed on a C(18) column by isocratic elution with acetonitrile-water, and UV detection at 260 nm. The amounts of adenosine, cordycepin, 2'-deoxyadenosine, guanosine and uridine in Cordyceps were 0.28-14.15, 0.006-6.36, 0.01-0.14, 0.68-14.79 and 0.19-20.29 mg/g, respectively. Among the nucleosides studied, cordycepin was characteristically included in Cordyceps militaris (L.) Link. (CM), which is one of key Cordyceps allies, and might be a good marker for authenticating CM. The ratio of nucleosides to adenosine contents in Cordyceps seemed to be a useful marker for authentication and quality control of Cordyceps.

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Section: Methods Validationmentioning

confidence: 61%

Section: Contents Of Nucleosides In Cordycepsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Simple HPLC‐UV determination of nucleosides and its application to the authentication of Cordyceps and its allies

Ikeda

Nishimura

Sun

et al. 2008

Biomedical Chromatography

View full text Add to dashboard Cite

show abstract

“…Subsequently, the precisely weighed powder (1.0 g) was mixed with approximately 20 mL of water, and then extracted ultrasonically for 1 h at room temperature (Guo et al 2006). The extract solution was filtrated through analytical filter paper.…”

Section: Methodsmentioning

confidence: 99%

Determination and comparative analysis of 13 nucleosides and nucleobases in natural fruiting body of Ophiocordyceps sinensis and its substitutes

et al. 2017

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Nucleosides and nucleobases are one of the most important indicators of quality control. A sensitive and reliable high performance liquid chromatography-ultraviolet method was applied to analyse 13 nucleosides and nucleobases simultaneously in 15 batches of nine Ophiocordyceps species and its allies in China. Principal component analysis (PCA) and cluster analysis were conducted by SPSS 22.0 software (IBM Corp., Armonk, NY, USA). The 15 samples of Cordyceps were differentiated successfully based on their nucleoside and nucleobase content. Total nucleosides content in mycelium was significantly higher than that in the natural fruiting bodies of Ophiocordyceps sinensis (NFOS). Five nucleosides or nucleobases – adenine (A), guanosine (Gu), uracil (U), uridine (Ur) and guanine (G) – were the major components contributed to the total variance according to PCA. The profiles of the 13 tested nucleosides and nucleobases (including adenosine, cytidine, guanosine, inosine, thymidine, uridine, cordycepin, adenine, cytosine, guanine, thymine, uracil and hypoxanthine) can discriminate different samples and can be candidate indicators applied for the quality control of Ophiocordyceps and its allies.

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Cathodic Stripping Voltammetry of Uracil. Experimental and Theoretical Study Under Conditions of Square‐Wave Voltammetry

2008

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The electrode mechanism of uracil at a hanging mercury drop electrode (HMDE) is studied under cathodic stripping square-wave voltammetric mode owing to the cathodic dissolution of a sparingly soluble compound formed between the electrode material and uracil. The experimental results can be partly explained in the light of the recent theory for cathodic stripping processes of insoluble salts under conditions of square-wave voltammetry. It is established that the electrode reaction is complicated by attractive interactions between the deposited species of the insoluble compound. To elucidate the electrode mechanism completely a novel theoretical model is developed considering adsorption of the reacting analyte and lateral interactions between species of the insoluble compound. With the help of numerical simulations the effect of interactions is studied in detail, emphasizing the properties of the response that can be used as diagnostic criteria for recognition of the type of interaction forces. Theoretically predicted voltammetric properties agree well with the experimental results enabling clarification of the complex electrode mechanism of uracil at HMDE.

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Identification and determination of nucleosides in Cordyceps sinensis and its substitutes by high performance liquid chromatography with mass spectrometric detection

Cited by 58 publications

References 14 publications

Simple HPLC‐UV determination of nucleosides and its application to the authentication of Cordyceps and its allies

Simple HPLC‐UV determination of nucleosides and its application to the authentication of Cordyceps and its allies

Determination and comparative analysis of 13 nucleosides and nucleobases in natural fruiting body of Ophiocordyceps sinensis and its substitutes

Cathodic Stripping Voltammetry of Uracil. Experimental and Theoretical Study Under Conditions of Square‐Wave Voltammetry

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