Identification and Determination of Total Flavonoids in Ethanol Extract of Old and Young Angsana Leaves (Pterocarpus indicus Willd.) Using Visible Spectrophotometry

Fernanda, María; Andriani, Rina; Estulenggani, Z.; Kusumo, Galuh Gondo

doi:10.5220/0007546605410544

Cited by 3 publications

(2 citation statements)

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“…In this study, the CSE was qualitatively analyzed for the presence of flavonoids, alkaloids, tannins, steroids, terpenoids, and saponins. The flavonoids were tested using Wilstater's test as described in the previous studies ( 23 24 ). Furthermore, the tannin presence was examined whether the black or blue color was performed after reacting the CSE with 1% ferric chloride ( 25 ).…”

Section: Methodsmentioning

confidence: 99%

Caesalpinia sappan reduces the stemness of breast cancer stem cells involving the elevation of intracellular reactive oxygen species

Jenie,

Amalina,

Hermawan

et al. 2023

Research in Pharmaceutical Sciences

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Background and purpose: Breast cancer stem cells (BCSCs) as a kind of tumor cells are able to regenerate themselves, leading to apoptosis resistance and cancer relapse. It was reported that BCSCs contain lower levels of reactive oxygen species (ROS) associated with stemness capability. Caesalpinia sappan has been proposed for its chemopreventive potency against several cancer cells. This study aimed to evaluate the effects of Caesalpinia sappan extract (CSE) on cytotoxicity, apoptosis induction, ROS generation, and stemness markers of MDA-MB-231 and its BCSCs. Experimental approach: Caesalpinia sappan was extracted under maceration with methanol. Magnetic-activated cell sorting was used to isolate BCSCs based on CD44+ and CD24- cell surface expression. The MTT test was used to assess the cytotoxic effects of CSE on MDA-MB-231 and BCSCs. Moreover, flow cytometry was used to examine the cell cycle distribution, apoptosis, ROS level, and CD44/CD24 level. Using qRT-PCR, the gene expression of the stemness markers NANOG, SOX-2, OCT-4, and c-MYC was assessed. Findings/Results: We found that MDA-MB-231 contains 80% of the BCSCs population, and CSE showed more potent cytotoxicity toward BCSCs than MDA-MB-231. CSE caused apoptosis in MDA-MB-231 and BCSCs cells by increasing the level of ROS. Furthermore, CSE significantly reduced the MDA-MB-231 stemness marker CD44+/CD24- and the mRNA levels of pluripotent markers of cells in BCSCs. Conclusion and implications: CSE potentially reduces BCSCs stemness, which may be mediated by the elevation of the ROS levels and reduction of the expression levels of stemness transcription.

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Section: Methodsmentioning

confidence: 99%

Caesalpinia sappan reduces the stemness of breast cancer stem cells involving the elevation of intracellular reactive oxygen species

Jenie,

Amalina,

Hermawan

et al. 2023

Research in Pharmaceutical Sciences

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“…The qualitative results are expressed as (+) for the presence and (−) for the absence of phytochemicals. The flavonoids were test using Wilstater's test according to (Fernanda et al, 2019). Briefly, 2 mg of each CL and PN was mixed with HCl (Merck, Darmstadt, Germany) 500µL and 0.02 mg magnesium (Merck, Darmstadt, Germany).…”

Section: Phytochemical Screening Of Curcuma Longa and Phyllanthus Nir...mentioning

confidence: 99%

Combination Curcuma longa and Phyllanthus niruri Extract Potentiate Antiproliferative in Triple Negative Breast Cancer MDAMB-231 Cells

Hermansyah

Paramita

et al. 2023

Asian Pac J Cancer Prev

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Background: Triple negative breast cancer cells (TNBC) are a small part of cancer-inducing cells in breast cancer, which are characterized by high metastatic and self-renewal. Self-renewal has the ability to renew itself and loses control of proliferation. Curcuma longa extract (CL) and Phyllanthus niruri extract (PN) known to have anti-proliferative effects on cancer cells. However, the effects of combination CL and PN on TNBC proliferation still unclear. Aims: This study aimed to evaluate the antiproliferative effects of the combination CL and PN on TNBC MDAMB-231 and attempted to elucidate the underlying molecular mechanisms. Subjects and Methods: The dried rhizomes of Curcuma longa and the herbs of Phyllanthus niruri were macerated with ethanol for 72 h.The antiproliferative and synergistic effects of combination CL and PN were investigated using 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay. Combination index values were calculated using CompuSyn (ComboSyn, Inc, Paramus, NJ). The cell cycle and apoptosis assay were determined by propidium iodide (PI) and PI-AnnexinV assay under flow cytometer, respectively. The intracellular ROS levels were evaluated using 2′,7′-Dichlorodihydrofluorescein diacetate (DCFDA) assay. The mRNA expressions of proliferation-related genes in the cells were determined using bioinformatic assay. Results: The CL and PN single treatment caused a potent and dose-dependent decrease in the percentage of viable cells with IC 50 value of 13 μg/mL and 45 μg/mL for 24 h, respectively. The combination index values of the different combinations ranged from 0.08 – 0.90, indicating slightly strong to very strong synergistic effects. The combination of CL and PN also remarkably induced the S- and G2/M-phases cell cycle arrest that leading to apoptosis induction. Furthermore, the combination of CL and PN treatment induced the intracellular reactive oxygen species (ROS) levels. Mechanistically, the AKT1, EP300, STAT3 and EGFR signaling as potential targets of combination CL and PN in antiproliferation and antimetastatic of TNBC. Conclusions: The combination of CL and PN exerted promising antiproliferative effects in TNBC. Therefore, CL and PN may be considered a potential source for the development of potent anticancer drugs for breast cancer treatment.

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Evaluation of biological potential of red onion skin extract for anticancer and antimicrobial activities

El Sadda,

El-Shobaky,

El Sharawy

et al. 2024

Process Biochemistry

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Identification and Determination of Total Flavonoids in Ethanol Extract of Old and Young Angsana Leaves (Pterocarpus indicus Willd.) Using Visible Spectrophotometry

Cited by 3 publications

References 0 publications

Caesalpinia sappan reduces the stemness of breast cancer stem cells involving the elevation of intracellular reactive oxygen species

Caesalpinia sappan reduces the stemness of breast cancer stem cells involving the elevation of intracellular reactive oxygen species

Combination Curcuma longa and Phyllanthus niruri Extract Potentiate Antiproliferative in Triple Negative Breast Cancer MDAMB-231 Cells

Evaluation of biological potential of red onion skin extract for anticancer and antimicrobial activities

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