Identification and differentiation of Asian seabass and mangrove red snapper fillets by CYTB sequence-based PCR analysis

Saetang, Jirakrit; Benjakul, Soottawat

doi:10.1007/s11694-022-01545-5

Cited by 3 publications

(1 citation statement)

References 30 publications

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“…Recently, Giantsis et al (2023) presented a multiplex PCR for identifying fish species of the Mullidae family using mitochondrial genes with target lengths ranging from 106 to 438 bp [31]. Similarly, Saetang and Benjakul (2022) proposed a cytochrome b sequencebased PCR for detecting Asian seabass and mangrove red snapper with amplicon lengths between 268 and 480 bp [32]. Wilwet et al (2021) reported another species-specific PCR with a 204-778 bp target to differentiate seven shrimp species [28].…”

Section: Laver Authentication Using Multiplex Pcrmentioning

confidence: 99%

Rapid and Simultaneous Authentication of Six Laver Species Using Capillary Electrophoresis-Based Multiplex PCR

Yang,

Kim,

Kim

et al. 2024

Foods

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Lavers are typically consumed in dried or seasoned forms. However, commercially processed lavers can lead to seafood fraud because it is impossible to authenticate the original species based on morphological characteristics alone. In this study, we developed a capillary electrophoresis-based multiplex polymerase chain reaction (PCR) to authenticate six different laver species. The species-specific primer sets to target the chloroplast rbcL or rbcS genes were newly designed. We successfully established both singleplex and multiplex conditions, which resulted in specific amplicons for each species (N. dentata, 274 bp; N. yezoensis, 211 bp; N. seriata, 195 bp; N. tenera, 169 bp; N. haitanensis, 127 bp; P. suborbiculata, 117 bp). Moreover, the assays were sensitive enough to detect DNA ranging from 10 to 0.1 pg of DNA. The optimized capillary electrophoresis-based multiplex PCR was successfully applied to 40 commercial laver products. In addition to detecting the laver species as stated on the commercial label, the assay discovered cases where less expensive species were mixed in. With its advantageous properties, such as short amplicon size, high specificity, and superior sensitivity, this assay could be used for the authentication of the six laver species.

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