Identification and differentiation of soft rot and blackleg bacteria from potato using nested and multiplex PCR

Ashmawy, Nader A.; El-Bebany, Ahmed F.; Shams, Amany H. M.; Shoeib, Alia A.

doi:10.1007/s41348-019-00257-1

Cited by 13 publications

(12 citation statements)

References 26 publications

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“…The use of 16S rRNA gene sequences to study bacterial phylogeny and taxonomy has been by far the most common housekeeping genetic marker (Ashmawy et al, 2020). 16S rRNAgene was used to identify the tested isolates and study the genetic variability among 3 isolates of Pantoea spp., 2 isolates of Pseudomonas spp.…”

Section: Discussionmentioning

confidence: 99%

Pathological, Biochemical and Molecular Characterization of the Seed-Borne Bacteria "Pantoea Spp., Xanthomonas Spp. And Pseudomonas Spp." From Solanaceous Plants in Egypt

Ashmawy¹,

Shoeib²,

Youssef³

et al. 2020

J microb biotech food sci

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The seed-borne bacterial diseases of family solanaceae cause significant economic losses worldwide. In the present study, fourteen bacterial isolates were recovered from seeds of different varieties of tomato, eggplant, black nightshade and tobacco. The seed samples were collected from different locations in Egypt. Isolated bacteria were identified based on morphological, physiological and biochemical tests as well as colonial morphology aspects on the differential medium, yeast extract dextrose calcium carbonate (YDC) besides the selective media (PA 20, King's B and Tween B). Pathogenicity of the isolated bacteria was assessed for causing graywall, bacterial speck and bacterial spot symptoms on tomato seedlings. Disease symptoms were recognized on leaves and stems of the inoculated seedlings. Re-isolation from collected seeds of the inoculated tomato plants was performed. Results indicated that Pantoea spp. was an endophytic bacteria, Pseudomonas spp. was an epiphytic bacteria, while Xanthomonas spp. was both epiphytic and endophytic bacteria. Furthermore, cellular fatty acids composition were identified and quantified to give a profile for tested bacterial isolates. Moreover, the bacterial isolates were identified at the molecular level via PCR reactions utilizing the 16S rRNA gene. Partial DNA sequences were analyzed using BLAST tool revealed that the inferred 16S rRNA partial sequences of the 7 isolates showed similarity to Pantoea ananatis (3 isolates), Pseudomonas syringae pv. tomato (2 isolates) and Xanthomonas vesicatoria (2 isolates). To the best of our knowledge the bacterium associated with the graywall seems to be first report of P. ananatis isolated from tomato fruits and black nightshade seeds in Egypt.

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Section: Discussionmentioning

confidence: 99%

Pathological, Biochemical and Molecular Characterization of the Seed-Borne Bacteria "Pantoea Spp., Xanthomonas Spp. And Pseudomonas Spp." From Solanaceous Plants in Egypt

Ashmawy¹,

Shoeib²,

Youssef³

et al. 2020

J microb biotech food sci

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“…No. LT592256 96 Pectobacterium atrosepticum Identified by specific primer (Y45,Y46), Bacterial Pl. Dis.…”

Section: Methodsmentioning

confidence: 99%

Nano-metals forming bacteria in Egypt. I. Synthesis, characterization and effect on some phytopathogenic bacteria in vitro

Zaki

Kamal

Ashmawy

et al. 2021

Sci Rep

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Bacterial metal reducers were isolated from water samples collected from harsh condition locations in Egypt. Four selected isolates were identified as Enterococcus thailandicus, Pseudomonas putida, Marinobacter hydrocarbonoclasticus, and P. geniculata for Copper (Cu), Iron (Fe), Cobalt (Co) and Zinc (Zn) Nanoparticles (NPs) production sequentially. Nitrate reductase enzyme was assayed for bacterial isolates which demonstrated that P. putida, and M. hydrocarbonoclasticus have the maximum enzyme production. The produced NPs were characterized by using XRD, TEM, UV–VIS spectroscopy. Magnetic properties for all selected metals NPs were measured using Vibrating Sample Magnetometer (VSM) and demonstrated that FeNPs recorded the highest magnetization value. The antibacterial activity of selected metals NPs was tested against some phytopathogenic bacteria causing the following diseases: soft rot (Pectobacterium carotovorum, Enterobacter cloacae), blackleg (Pectobacterium atrosepticum and Dickeya solani), brown rot (Ralstonia solanacearum), fire blight (Erwinia amylovora) and crown gall (Agrobacterium tumefaciens). All metals NPs showed an antagonistic effect against the tested isolates, particularly, FeNPs showed the highest antibacterial activity followed by CuNPs, and ZnNPs. Due to the small size, high reactivity, and large surface area of biologically synthesized NPs, they are used as a good disinfector, and can be considered as a new and alternative approach to traditional disease management methods.

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“…The blackleg disease is responsible for the rotting and wilting of stems on growing potato plants. Dickeya solani is a complex disease in Egypt that causes bacterial soft rot/blackleg in potato crops 27 . The symptoms of D. solani are often indistinguishable from those caused by Pectobacterium atrosepticum, D. solani is more virulent as causing disease at lower levels of inoculum, as well as spreading through the plant more effectively 28 .…”

Section: Introductionmentioning

confidence: 99%

Nano-Metals Forming Bacteria in Egypt. II. Efficacy towards Biomolecules, Ultrastructure, Growth Parameters, and Eco-Friendly Therapeutic of Soft Rot/Blackleg Genera

Shoeib

Ashmawy

Kamal

et al. 2022

Preprint

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The nanoparticles (NPs) formed by Enterococcus thailandicus, Pseudomonas putida, Marinobacter hydrocarbonoclasticus, and P. geniculate were tested against soft rot/blackleg genera. The effects of NPs recorded on bacterial DNA, proteins, and carbohydrates concentration of Pectobacterium carotovorum subsp. carotovorum, Enterobacter cloacae (soft rot), and Dickeya solani (soft rot/blackleg). Treated cells showed degradation in isolated DNA and decreased proteins and carbohydrates concentration compared with untreated cells. Using Scanning Electron Microscope (SEM), the treated cells showed collapsed and small pits in the cell wall. Using Transmission Electron Microscope (TEM), internal changes showed penetration of NPs inside the tested bacterial cells, the appearance of periplasmic space, formation of vacuoles, and condensation of cytoplasm. Disease severity ex vivo of potato tuber infected with tested genera demonstrated that NPs treatment didn't show any rotted tissue compared with untreated. The ability to uptake and accumulate FeNPs from the soil in potato (Solanum tuberosum) seedlings; Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES) was used. ICP-OES recorded an increase in iron content of treated potato (Solanum tuberosum) seedlings with NPs, compared with untreated. FeNPs can be used to control soft rot/blackleg diseases; instead of copper pesticides. It could be a new, and alternative approach to traditional disease management methods and increase the plant's nutritional value.

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Identification and differentiation of soft rot and blackleg bacteria from potato using nested and multiplex PCR

Cited by 13 publications

References 26 publications

Pathological, Biochemical and Molecular Characterization of the Seed-Borne Bacteria "Pantoea Spp., Xanthomonas Spp. And Pseudomonas Spp." From Solanaceous Plants in Egypt

Pathological, Biochemical and Molecular Characterization of the Seed-Borne Bacteria "Pantoea Spp., Xanthomonas Spp. And Pseudomonas Spp." From Solanaceous Plants in Egypt

Nano-metals forming bacteria in Egypt. I. Synthesis, characterization and effect on some phytopathogenic bacteria in vitro

Nano-Metals Forming Bacteria in Egypt. II. Efficacy towards Biomolecules, Ultrastructure, Growth Parameters, and Eco-Friendly Therapeutic of Soft Rot/Blackleg Genera

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