Identification and dipeptidyl peptidase IV (DPP-IV) inhibitory activity verification of peptides from mouse lymphocytes

Impact of Lactiplantibacillus plantarum and casein fortification on angiotensin converting enzyme inhibitory peptides in yogurt: identification and in silico analysis

“…Peptides with scores above 0.8 were considered potential ACEIPs. 40 The specific results are presented in Table 5. It is noteworthy that NILRFF obtained a significantly high PeptideRanker score of 0.93, suggesting the possibility of having a strong ACE inhibitory activity.…”

Characterization of Allium tuberosum rottl. Peptides with Dual Inhibitory Activities against Angiotensin I Converting Enzyme and Dipeptidyl Peptidase-IV

“…For instance, four DPP-IV inhibitory peptides derived from quinoa hydrolysate with potential IC50 values (IPI: 5.25 μM; IPV: 26.15 μM; VAYPL: 42.93 μM; IPIN: 56.58 μM) have been identified as competitive inhibitors [27]. Moreover, DPP-IV competitive inhibitory peptides were also determined in napin of rapeseed (Brassica napus) hydrolysate (IPQVS) [28], camel whey protein (VPV, YPI, VPF, EPVK, LAHKPL, YPLR) [29], and mouse lymphocytes (SGVSLAALKKALAAAGYDVEK) [30]. On the other hand, peptide NY-7 was found to be a noncompetitive inhibitor of DPP-IV, as its Km value remained stable in both situations, whether NY-7 was present or absent (Supplementary data Table S1 and Figure 4D).…”

“…It suggests that NY-7 has been interacted with DPP-IV receptor via the second binding sites instead of directly binding with the active sites. Until now, the DPP-IV noncompetitive inhibitors were widely common, such as peptides obtained from αlactalbumin-rich whey hydrolysate (LDQWLCEKL, IC50: 131 µM) [31], Atlantic salmon skin hydrolysate (VLATSGPG, IC50: 1.73 mM) [32], and mouse lymphocytes (SAPRHGSLGFLPRK, IC50: 23.443 mg/L) [30]. These findings clarify the inhibitory mechanisms of LY-5 and NY-7 against DPP-IV, demonstrating their potential as therapeutic agents for diseases such as diabetes.…”