Identification and distinction of acrolein‐deoxyguanosine adduct isomers by high‐performance liquid chromatography/ion mobility spectrometry/quadrupole time‐of‐flight mass spectrometry combined with in‐source collision‐induced dissociation

Abstract

The acrolein-deoxyguanosine (Acr-dG) adducts α-[Acr+dG] and γ-[Acr+dG] (Figure 1), potential mutagens, have been widely studied. 1-3 The mutagenicity of α-[Acr+dG] is stronger than that of γ-[Acr+dG]. If a sufficient amount of the pure compound can be obtained, nuclear magnetic resonance (NMR) spectroscopy is typically used to identify these two isomers. This technique inevitably results in the consumption of a large amount of raw material and low detection sensitivity, and the elucidation of the NMR spectra can be time consuming. In addition, during the production of α-[Acr+dG] and γ-[Acr+dG], preparative high-performance liquid chromatography with