Identification and diversity of bovine major histocompatibility complex class II haplotypes in Japanese Black and Holstein cattle in Japan

Abstract

Bovine leukocyte antigen (BoLA), the major histocompatibility complex of cattle, is one of the most polymorphic gene clusters. We genotyped a population of 109 Japanese Black and 39 Holstein cattle to analyze their BoLA class II haplotypes, BoLA-DRB3 locus, 5 BoLA-DQA loci, and 5 BoLA-DQB loci. We identified 26 previously reported DRB3 alleles, 22 previously reported and 3 new DQA alleles, and 24 previously reported and 6 new DQB alleles. A dendrogram was constructed based on the predicted amino acid sequences of the α1 or β1 domains encoded by BoLA-DQA or -DQB alleles, which revealed that DQA alleles were clustered into 5 loci, whereas DQB alleles could not be clearly assigned to specific DQB loci. The BoLA-DRB3-DQA-DQB haplotypes were sorted by sequential analytical processes, and 42 distinct haplotypes, including 11 previously published haplotypes and 31 novel haplotypes, were defined. Strong linkage disequilibrium was present in the BoLA genes. We also compared DRB3-DQA1 haplotype frequencies between 507 Japanese Black and 143 Holstein cattle. Thirty-nine DRB3-DQA1 haplotypes were identified, including 29 haplotypes from Japanese Black and 22 haplotypes from Holstein cattle. The majority of the haplotypes could be identified in both breeds, although several haplotypes were identified in only a single breed. This is the first report presenting a detailed study of the BoLA class II haplotype in Japanese Black and Holstein cattle in Japan.

Assessment of biodiversity in Chilean cattle using the distribution of major histocompatibility complex class IIBoLA‐DRB3 allele

“…Similarly, many studies have shown that BoLA class II allele frequencies differ between cattle breeds. For example, allele frequencies of the BoLA-DRB3 gene differ between Jersey, Holstein, Argentine Creole, Japanese Shorthorn, Brazilian dairy Gir, and four Japanese breeds (7)(8)(9)(10)(11)(12)(13)(14). Recently, a PCR sequence-based typing (SBT) method was used to genotype BoLA-DRB3, and it showed greater discriminatory power and precision than PCR-restriction fragment length polymorphism (RFLP) for estimating BoLA-DRB3 diversity in cattle breeds (15).…”

Major Histocompatibility Complex-Associated Resistance to Infectious Diseases: The Case of Bovine Leukemia Virus Infection

“…From this cluster, at least 61 BoLA DQA and 81 BoLA DQB alleles have been described and listed in the IPD-MHC database, and 56 different haplotypes were described in Japanese Black and Holstein cattle [35]. The high number of different alleles, both on the DR and DQ loci, suggests that DR and DQ molecules complement each other for the presentation of a broad spectrum of antigens in cattle.…”

Bovine leukemia virus: current perspectives

“…143,[147][148][149][150][151][152] BoLA-DRB3*0902 animals with LPL phenotype did not transmit the infection at herd level in natural conditions, 48 and under experimental conditions, it was necessary to inoculate a large volume of blood in order to infect sheep. 145,147 Furthermore, to date, the BoLA-DRB3*0902 allele has not been associated with susceptibility to other infectious agent, nor has it negatively affected production or reproduction traits.…”