Identification and enrichment of colony-forming cells from the adult murine pituitary

Lepore, Diana A.; Roeszler, Kelly N.; Wagner, Josef; Ross, Shelley; Bauer, Karl; Thomas, Paul Q.

doi:10.1016/j.yexcr.2005.04.023

Cited by 68 publications

(95 citation statements)

References 42 publications

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“…Vankelecom and colleagues isolated murine pituitary cells from side populations that can be clonally expanded and share many stem cell characteristics Krylyshkina et al, 2005;Vankelecom, 2007). Other laboratories also identified pituitary colony-forming cells and their multipotent differentiation capabilities (Lepore et al, 2005(Lepore et al, , 2006Mogi et al, 2005). Although propagating cells or cell lines that share certain stem-like cell markers had been isolated from pituitary tumours (Danila et al, 2000;Horvath and Kovacs, 2002;Abbud et al, 2005), stringent functional characterisation of pituitary tumour stem-like cells has been scarce.…”

Section: Pascs Initiated Pituitary Tumours That Are Serially Transplamentioning

confidence: 99%

Isolation of tumour stem-like cells from benign tumours

et al. 2009

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BACKGROUND: Cancerous stem-like cells (CSCs) have been implicated as cancer-initiating cells in a range of malignant tumours. Diverse genetic programs regulate CSC behaviours, and CSCs from glioblastoma patients are qualitatively distinct from each other. The intrinsic connection between the presence of CSCs and malignancy is unclear. We set out to test whether tumour stem-like cells can be identified from benign tumours. METHODS: Tumour sphere cultures were derived from hormone-positive and -negative pituitary adenomas. Characterisation of tumour stem-like cells in vitro was performed using self-renewal assays, stem cell-associated marker expression analysis, differentiation, and stimulated hormone production assays. The tumour-initiating capability of these tumour stem-like cells was tested in serial brain tumour transplantation experiments using SCID mice. RESULTS: In this study, we isolated sphere-forming, self-renewable, and multipotent stem-like cells from pituitary adenomas, which are benign tumours. We found that pituitary adenoma stem-like cells (PASCs), compared with their differentiated daughter cells, expressed increased levels of stem cell-associated gene products, antiapoptotic proteins, and pituitary progenitor cell markers. Similar to CSCs isolated from glioblastomas, PASCs are more resistant to chemotherapeutics than their differentiated daughter cells.

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Section: Pascs Initiated Pituitary Tumours That Are Serially Transplamentioning

confidence: 99%

Isolation of tumour stem-like cells from benign tumours

et al. 2009

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“…A side population that efficiently excludes the Hoechst 33342 dye has been shown to segregate with sphereforming cells in the pituitary (Chen et al 2005). Pituitary colony-forming cells that display notable clonogenic potential have also been isolated (Lepore et al 2005). More recently, stem-cell specific markers such as SOX2C, SOX9, or OCT4 in addition to other epithelial markers have been found in a single-cell layer in the marginal zone suggesting the presence of stem/progenitor cells that may contribute to cell renewal in the adult pituitary (Fauquier et al 2008, Garcia-Lavandeira et al 2008, Gleiberman et al 2008.…”

Section: Introductionmentioning

confidence: 99%

Cell cycle control of pituitary development and disease

Quereda

Malumbres²

2008

Journal of Molecular Endocrinology

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The pituitary gland regulates diverse physiological functions, including growth, metabolism, reproduction, stress response, and ageing. Early genetic models in the mouse taught us that the pituitary is highly sensitive to genetic alteration of specific cell cycle regulators such as the retinoblastoma protein (pRB) or the cell cycle inhibitor p27 Kip1 . The molecular analysis of human pituitary neoplasias has now corroborated that cell cycle deregulation is significantly implicated in pituitary tumorigenesis. In particular, proteins involved in cyclin-dependent kinase regulation or the pRB pathway are altered in nearly all human pituitary tumors. Additional cell cycle regulators such as PTTG1/securin may have critical roles in promoting genomic instability in pituitary neoplasias. Recent experimental data suggest that these cell cycle regulators may have significant implications in the biology of putative progenitor cells and pituitary homeostasis. Understanding how cell cycle regulation controls pituitary biology may provide us with new therapeutic approaches against pituitary diseases.

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“…FS cells are enriched in pituitary fractions with colony-forming abilities, from which sporadic somatotroph differentiation has been reported both in vitro (12) and in vivo (13). However, FS cells are heterogeneous, representing several types of cells with diverse morphology and gene expression, and convincing evidence for their role as multipotent progenitors is lacking (14)(15)(16).…”

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confidence: 99%

SOX2-expressing progenitor cells generate all of the major cell types in the adult mouse pituitary gland

Fauquier¹,

Rizzoti

Dattani³

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

342

447

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The pituitary gland adapts the proportion of each of its endocrine cell types to meet differing hormonal demands throughout life. There is circumstantial evidence that multipotent adult progenitor cells contribute to this plasticity, but these cells have not been identified. Here, we describe a small (<0.05%) population of progenitor cells in the adult pituitary gland. We show that these cells express SOX2, a marker of several early embryonic progenitor and stem cell types, and form ''pituispheres'' in culture, which can grow, form secondary spheres, and differentiate to all of the pituitary endocrine cell types, as well as folliculostellate cells. Differentiation of cells in the pituispheres was associated with the expression of nestin, SOX9, and S100. Cells expressing SOX2 and E-cadherin are found throughout Rathke's pouch (RP) in embryos but persist in the adult gland, mostly in a narrow zone lining the pituitary cleft, but also are scattered throughout the pituitary. However, unlike in embryonic RP, most of these SOX2 ؉ cells in the adult gland also express SOX9 and S100. We suggest that this SOX2 ؉ /SOX9 ؉ population represents transit-amplifying cells, whereas the SOX2 ؉ /SOX9 ؊ cells we identify are multipotent progenitor/stem cells persisting in the adult pituitary.stem cell ͉ folliculostellate cells

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Identification and enrichment of colony-forming cells from the adult murine pituitary

Cited by 68 publications

References 42 publications

Isolation of tumour stem-like cells from benign tumours

Isolation of tumour stem-like cells from benign tumours

Cell cycle control of pituitary development and disease

SOX2-expressing progenitor cells generate all of the major cell types in the adult mouse pituitary gland

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