Identification and Evaluation of Antiepileptic Activity of C<sub>21</sub> Steroidal Glycosides from the Roots of <i>Cynanchum wilfordii</i>

Li, Jinlong; Gao, Zhaobing; Zhao, Weimin

doi:10.1021/acs.jnatprod.5b00766

Cited by 44 publications

(33 citation statements)

References 34 publications

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“…5), which suggested that metabolismdependent ROS production may be the major reason for the rise in FSC and shape alteration. MTE, in our experiments, was a crude extract containing abundant C 21 steroids with anti-cancer activity, which have been previously extracted from several kinds of herbal medicine and have presented cytotoxicity in various cancer cell lines (Li et al, 2016;Yin et al, 2016). From M. tenacissimae, more than 40 kinds of C-21 steroidal glycosides have been isolated (Zhang et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Cytotoxicity of anti-tumor herbal Marsdeniae tenacissimae extract on erythrocytes

Hao

Chen

et al. 2017

J. Zhejiang Univ. Sci. B

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Marsdeniae tenacissimae extract (MTE) has been used as an adjuvant medicine for cancer therapy for a long time. Although massive studies demonstrated its considerable anti-cancer effect, there is no research on its influence on erythrocytes, which are firstly interacted with MTE in the circulation. To investigate the influence of MTE on erythrocytes, we used a flow cytometer to detect the MTE-treated alternations of morphology, calcium concentration, and reactive oxygen species (ROS) level in erythrocytes. We used hemolysis under different osmotic solutions to evaluate the fragility of erythrocytes. Data showed that MTE treatment dose-dependently increased the ratio of erythrocyte fragmentation (P<0.001) and shrinking, and elevated the forward scatter (FSC) value (P<0.001) and calcium accumulation (P<0.001). MTE induced ROS production of erythrocytes under the high glucose condition (P<0.01) and consequently caused a rise in fragility (P<0.05). These results suggest that MTE induces cytotoxicity and aging in erythrocytes in a dose-dependent manner, and presents the possibility of impairment on cancer patients' circulating erythrocytes when MTE is used as an anti-cancer adjuvant medicine.

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Section: Discussionmentioning

confidence: 99%

Cytotoxicity of anti-tumor herbal Marsdeniae tenacissimae extract on erythrocytes

Hao

Chen

et al. 2017

J. Zhejiang Univ. Sci. B

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“…This study revealed that CAR was incorrectly used in eight of the 13 products distributed as CWR, including possible confusion of CWR and CAR [ 5 ]. Previous phytochemical investigations of CWR identified the presence of pregnane glycosides, acetophenones, and humulanolides [ 6 , 7 , 8 , 9 , 10 , 11 ]. Although there are several reports on ingredient research using materials of CWA and CAR available on the market, they may not be of the precise species.…”

Section: Introductionmentioning

confidence: 99%

Preliminary Quality Evaluation and Characterization of Phenolic Constituents in Cynanchi Wilfordii Radix

et al. 2018

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A new phenolic compound, 2-O-β-laminaribiosyl-4-hydroxyacetophenone (1), was isolated from Cynanchi Wilfordii Radix (CWR, the root of Cynanchum wilfordii Hemsley), along with 10 known aromatic compounds, including cynandione A (2), bungeisides-C (7) and –D (8), p-hydroxyacetophenone (9), 2′,5′-dihydroxyacetophenone (10), and 2′,4′-dihydroxyacetophenone (11). The structure of the new compound (1) was elucidated using spectroscopic methods and chemical methods. The structure of cynandione A (2), including a linkage mode of the biphenyl parts that remained uncertain, was unambiguously confirmed using the 2D 13C–13C incredible natural abundance double quantum transfer experiment (INADEQUATE) spectrum. Additionally, health issues related to the use of Cynanchi Auriculati Radix (CAR, the root of Cynanchum auriculatum Royle ex Wight) instead of CWR have emerged. Therefore, constituents present in methanolic extracts of commercially available CWRs and CARs were examined using UV-sensitive high-performance liquid chromatography (HPLC), resulting in common detection of three major peaks ascribed to cynandione A (2), p-hydroxyacetophenone (9), and 2′,4′-dihydroxyacetophenone (11). Thus, to distinguish between these ingredients, a thin-layer chromatography (TLC) method, combined with only UV irradiation detection, focusing on wilfosides C1N (12) and K1N (13) as marker compounds characteristic of CAR, was performed. Furthermore, we propose this method as a simple and convenient strategy for the preliminary distinction of CWR and CAR to ensure the quality and safety of their crude drugs.

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“…Bunge Auriculate Root (Baishouwu) is an appellative name for the root tubers of Cynanchum auriculatum, Cynanchum bungei , and Cynanchum wilfordii in China, which is a famous tonic drug in TCM and known for its functions in enriching vital essence and enhancing immunity (Shan et al, 2006 ). Pharmacological studies suggested that Bunge Auriculate Root exhibits antitumor, antidepressant, anti-inflammatory, and antiepileptic activities (Peng et al, 2008 ; Yang et al, 2011 , 2014 ; Li et al, 2016 ). In addition, the root of Cynanchum otophyllum (Qingyangshen) has antifungal and antiepileptic activities (Zhao et al, 2007 ).…”

Section: Introductionmentioning

confidence: 99%

Inspecting the True Identity of Herbal Materials from Cynanchum Using ITS2 Barcode

2017

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Cynanchum is a large genus with some important medicinal species in China. The medicinal species in Cynanchum are easily confused, leading to potential safety risks. In this study, the internal transcribed spacer 2 (ITS2) barcode was used to discriminate the medicinal plants in Cynanchum. The identifying capability of ITS2 was assessed using the specific genetic divergence, BLAST1, neighbor-joining (NJ) tree, maximum-likelihood (ML) tree, and single-nucleotide polymorphism (SNP) methods. Results indicated that the intra-specific genetic divergences of Cynanchum species were lower than their inter-specific genetic divergences. Of the 87 samples from 17 species, ITS2 showed a high identification efficiency of 90.8 and 87.4% at the species level through BLAST1 and the nearest distance methods. NJ tree and ML tree also demonstrated the suitability of ITS2 to differentiate Cynanchum species. Meanwhile, a stable SNP was found, and it could accurately authenticate Cynanchum paniculatum and Cynanchum atratum. Furthermore, we collected 64 commercial samples from three commonly used herbal medicines and evaluated the capability of ITS2 to survey their authentication. Of these samples, Cynanchi Atrati Radix et Rhizoma (Baiwei) showed a potential safety problem, and all the 11 test samples were adulterants. In conclusion, ITS2 can distinguish medicinal species in Cynanchum effectively, and its application could greatly improve the identification efficiency and accuracy of commercial herbal medicines in this genus.

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Identification and Evaluation of Antiepileptic Activity of C₂₁ Steroidal Glycosides from the Roots of Cynanchum wilfordii

Cited by 44 publications

References 34 publications

Cytotoxicity of anti-tumor herbal Marsdeniae tenacissimae extract on erythrocytes

Cytotoxicity of anti-tumor herbal Marsdeniae tenacissimae extract on erythrocytes

Preliminary Quality Evaluation and Characterization of Phenolic Constituents in Cynanchi Wilfordii Radix

Inspecting the True Identity of Herbal Materials from Cynanchum Using ITS2 Barcode

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Identification and Evaluation of Antiepileptic Activity of C21 Steroidal Glycosides from the Roots of Cynanchum wilfordii

Cited by 44 publications

References 34 publications

Cytotoxicity of anti-tumor herbal Marsdeniae tenacissimae extract on erythrocytes

Cytotoxicity of anti-tumor herbal Marsdeniae tenacissimae extract on erythrocytes

Preliminary Quality Evaluation and Characterization of Phenolic Constituents in Cynanchi Wilfordii Radix

Inspecting the True Identity of Herbal Materials from Cynanchum Using ITS2 Barcode

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Product

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Identification and Evaluation of Antiepileptic Activity of C₂₁ Steroidal Glycosides from the Roots of Cynanchum wilfordii