Identification and evaluation of Galleria mellonella peptides with antileishmanial activity

Patiño-Márquez, Isabel A.; Patiño-González, Edwin; Hernández-Villa, Laura; Ortiz-Reyes, Blanca; Manrique-Moreno, Marcela

doi:10.1016/j.ab.2018.01.029

Cited by 13 publications

(6 citation statements)

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“…Cecropins anti-microbial peptides, initially found in the hemolymph of Hyalophora cecropia, are a main part of the innate immunity of insects [84]. They are also produced naturally by other kinds of insects (Galleria mellonella, tsetse flies, Drosophila) after bacterial infections [85,86]. They are linear cationic peptides having a sequence of 31-37 amino acids that differ greatly depending on the species [84].…”

Section: Cecropin 221 Cecropin and Leishmaniamentioning

confidence: 99%

“…Cecropin A can be purchased from Peninsula Laboratories (Belmont, CA, USA) [81]. Cecropin-D (Table 1), which is Cecropin-A homologous, has shown reduction of the parasite growth of Leishmania (V) panamensis promastigotes by 57% when the concentration was 100 µM [86]. In another study, Cecropin-A extracted from Hyalophora and Drosophila (Table 1) was demonstrated to inhibit the Leishmania aethiopica growth (amastigotes and promastigotes), with lower concentrations against amastigotes (50% inhibition at concentrations~0.250 mg/mL).…”

Section: Cecropin 221 Cecropin and Leishmaniamentioning

confidence: 99%

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Activity of Anti-Microbial Peptides (AMPs) against Leishmania and Other Parasites: An Overview

et al. 2021

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Anti-microbial peptides (AMPs), small biologically active molecules, produced by different organisms through their innate immune system, have become a considerable subject of interest in the request of novel therapeutics. Most of these peptides are cationic-amphipathic, exhibiting two main mechanisms of action, direct lysis and by modulating the immunity. The most commonly reported activity of AMPs is their anti-bacterial effects, although other effects, such as anti-fungal, anti-viral, and anti-parasitic, as well as anti-tumor mechanisms of action have also been described. Their anti-parasitic effect against leishmaniasis has been studied. Leishmaniasis is a neglected tropical disease. Currently among parasitic diseases, it is the second most threating illness after malaria. Clinical treatments, mainly antimonial derivatives, are related to drug resistance and some undesirable effects. Therefore, the development of new therapeutic agents has become a priority, and AMPs constitute a promising alternative. In this work, we describe the principal families of AMPs (melittin, cecropin, cathelicidin, defensin, magainin, temporin, dermaseptin, eumenitin, and histatin) exhibiting a potential anti-leishmanial activity, as well as their effectiveness against other microorganisms.

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Section: Cecropin 221 Cecropin and Leishmaniamentioning

confidence: 99%

Section: Cecropin 221 Cecropin and Leishmaniamentioning

confidence: 99%

Activity of Anti-Microbial Peptides (AMPs) against Leishmania and Other Parasites: An Overview

et al. 2021

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“…Galleria mellonella is increasingly used for a variety of microbiological applications, the most common being studying the virulence of fungal and bacterial pathogens (1, 67), and, to a lesser extent, studying viruses and other parasites (68–71). As cited above, many studies also aim at evaluating the antimicrobial activity of combinations of antibiotics or other antimicrobial compounds, as well as probiotic bacteria.…”

Section: Performing Infections and Further Inoculationsmentioning

confidence: 99%

Overview of rearing and testing conditions and a guide for optimizing Galleria mellonella breeding and use in the laboratory for scientific purposes

Pereira

Rossi

2020

APMIS

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Pereira MF, Rossi CC. Overview of rearing and testing conditions and a guide for optimizing Galleria mellonella breeding and use in the laboratory for scientific purposes. APMIS 2020; 128: 607-620. The greater wax moth Galleria mellonella is an increasingly popular and consolidated alternative infection model to assess microbial virulence and the effectiveness of antimicrobial compounds. The lack of G. mellonella suppliers aiming at scientific purposes and a lack of well-established protocols for raising and testing these animals may impact results and reproducibility between different laboratories. In this review, we discuss the state of the art of rearing the larvae in situ, providing an overview of breeding and testing conditions commonly used and their influence on larval health and experiments results, from setting up the environment, providing the ideal diet, understanding the effects of pretreatments, choosing the best testing conditions, to exploring the most from the results obtained. Meanwhile, we guide the reader through the most practical ways of dealing with G. mellonella to achieve successful experiments.

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“…G. mellonella larva has many advantages, including being widely available, procurable at a low cost, having no costs associated with breeding, no need for cultures or colonies to be preserved, and no need for other specialized equipment to house the larvae (Desbois and Coote 2012). Patiño-Márquez et al (2018) noted for the first time the anti-parasitic potential of peptides derived from the hemolymph of G. mellonella, such as anionic peptide 2 and cecropin-D peptides. El-Dirany et al (2021) identified the major families of anti-leishmanial AMPs (cecropin, melittin, defensin, cathelicidin, temporin, magainin, eumenitin, dermaseptin, and histatin), as well as their potency against other pathogens.…”

Section: Introductionmentioning

confidence: 99%

In vitro antiprotozoal effect of alcoholic extract of hemolymph of Galleria mellonella larva against Trichomonas gallinae

2022

Int J Vet Sci

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Trichomonas gallinae is a protozoan parasite that lives in the upper gastrointestinal tract of birds. Recently, the hemolymph of Galleria mellonella has been widely used as an anti-parasitic drug. The present work aimed to investigate the inhibitory activity of an alcoholic extract of G. mellonella hemolymph (AEGmH) on T. gallinae in vitro compared with that of metronidazole using wet smear and vital stain acridine orange (AO). The results showed that the degree of growth inhibition of Trichomonas was based on the AEGmH concentration. Furthermore, the highest reduction of parasitic growth (100%) was observed in cultures treated with 25, 12.5, and 6.25mg/mL AEGmH before 24h. Moreover, the same results were detected in cultures treated with 3.1 and 1.6mg/mL AEGmH, but after 48h, and doses of AEGmH (0.8 and 0.4mg/mL) after 96h. In comparison, complete inhibition of parasite growth (100%) was obtained by metronidazole at 25mg/mL after 72h. Besides, light microscopy observations revealed changes in trophozoites' morphology in culture treated with AEGmH. We concluded that the alcoholic extract of hemolymph of G. mellonella is suitable to counter trichomoniasis in vitro and showed antiprotozoal potential.

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Identification and evaluation of Galleria mellonella peptides with antileishmanial activity

Cited by 13 publications

References 46 publications

Activity of Anti-Microbial Peptides (AMPs) against Leishmania and Other Parasites: An Overview

Activity of Anti-Microbial Peptides (AMPs) against Leishmania and Other Parasites: An Overview

Overview of rearing and testing conditions and a guide for optimizing Galleria mellonella breeding and use in the laboratory for scientific purposes

In vitro antiprotozoal effect of alcoholic extract of hemolymph of Galleria mellonella larva against Trichomonas gallinae

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