Identification and Evaluation of Long Noncoding RNAs in Response to Handling Stress in Red Cusk-Eel (Genypterus chilensis) via RNA-seq

Temperature effect on oxidative stress and egg quality‐related genes on post‐ovulatory eggs and ovary of red cusk‐eel (Genypterus chilensis)

“…In this sense, it could be necessary to evaluate nontraditional egg‐quality markers that contribute to the development and control of hatchery systems for G. chilensis . A novel option to the discovery of egg‐quality markers in future studies could be the noncoding RNAs, considering that recently a large number of long‐noncoding RNAs has been described for this species (Dettleff et al ., 2020a) and that noncoding RNAs have been previously associated with egg quality in other teleost species (Ma et al ., 2012, 2015).…”

“…A novel option to the discovery of egg-quality markers in future studies could be the noncoding RNAs, considering that recently a large FISH number of long-noncoding RNAs has been described for this species (Dettleff et al, 2020a) and that noncoding RNAs have been previously associated with egg quality in other teleost species (Ma et al, 2012(Ma et al, , 2015.…”

“…The Ct values and efficiency were used to estimate expression values or quantities ( Q ) using the delta‐Ct method according the described by (Vandesompele et al ., 2002), which transforms expression values to Q values using the formula Q = E (minCt − sampleCt) . Normalization of target gene Q values was performed with the geometric mean of two reference genes (beta‐actin [ actb ] and TATA‐box binding protein associated factor 12 [ taf12 ]) validated for G. chilensis (Dettleff et al ., 2020a). For the statistical analysis on the post‐ovulatory eggs assay, an ANOVA test with a Fisher's LSD test posterior test was performed to identify significant differences ( P value < 0.05) on the oxidative damage and gene expression data, and a Kruskal–Wallis test was performed when corresponds for nonparametric data ( P value < 0.05).…”

Integrated lncRNA and mRNA Transcriptome Analyses in the Ovary of Cynoglossus semilaevis Reveal Genes and Pathways Potentially Involved in Reproduction

“…Additionally, several lncRNAs have been filtered and shown to regulate the synthesis of progesterone, oogenesis and oocyte maturation in goats ( Liu Y. et al, 2018 ). The identification of lncRNAs involved in processes such as growth and development ( Ali et al, 2018 ; Wu et al, 2020 ), stress responses ( Dettleff et al, 2020 ; Quan et al, 2020 ), immune responses ( Liu et al, 2019 ; Zheng et al, 2021 ), and sex differentiation ( Cai et al, 2019 ; Feng et al, 2020 ) in fish species has begun to be reported in recent years. However, studies involving the systematic identification and characterization of lncRNAs or the roles of lncRNAs in tongue sole reproduction are lacking.…”

Identification and Expression Analysis of Long Non-coding RNA in Large Yellow Croaker (Larimichthys crocea) in Response to Cryptocaryon irritans Infection

“…Because of the various functions, non-coding transcriptome profiling analysis has been performed in multiple species, such as mice (Josset et al, 2014), chicken (You et al, 2019), goat (Liu et al, 2018), sheep (Bakhtiarizadeh and Salami, 2019), silkworm (Bombyx mori) (Wu et al, 2016), and Pacific Oyster (Crassostrea gigas) (Sun and Feng, 2018). In teleost, similar works have been performed in red cusk-eel (Genypterus chilensis) (Dettleff et al, 2020), common carp (Cyprinus carpio) (Song et al, 2019), tilapia (Li et al, 2018), rainbow trout (Oncorhynchus mykiss) (Ali et al, 2018), and zebrafish (Pauli et al, 2012).…”