Identification and Expression Analysis of Zebrafish (Danio rerio) E-Selectin during Embryonic Development

Abstract

Abstract:In this study, we cloned the full-length cDNA of E-selectin of zebrafish (Danio rerio), analyzed its expression pattern and preliminarily explored its biological function. Zebrafish E-selectin cDNA is 3146 bp and encodes a putative 871 amino acid protein. All structural domains involved in E-selectin function are conserved in the putative protein. Whole-mount in situ hybridization of zebrafish at 24 and 48 h post-fertilization (hpf) revealed E-selectin expression mainly in vascular/endothelial progenitor cells in the posterior trunk and blood cells in the intermediate cell mass and posterior cardinal vein regions. Real-time quantitative RT-PCR analysis detected E-selectin expression at 0.2, 24 and 48 hpf and significantly decreased from 48 to 72 hpf. The expression of E-selectin, tumor necrosis factor-α and interleukin-1β was significantly upregulated at 22 to 72 h after induction with bacterial lipopolysaccharide. Thus, the structure of E-selectin protein is highly conserved among species, and E-selectin may be involved in embryonic development and essential for hematopoiesis and angiogenesis during embryonic development in zebrafish. Furthermore, we provide the first evidence of inflammatory mediators inducing E-selectin expression in non-mammalian vertebrates, which suggests that zebrafish E-selectin may be involved in inflammation and probably has similar biological function to mammalian E-selectin.

Dietary inosine and uracil efficacy on immune and growth genes expression, physiological performance and intestinal topography of juvenile sea bream ( Sparus aurata )

“…to mammalian E-selectins (Sun et al, 2015). In the present study, E-selectine gene expressed in a dose-dependent manner in inosine supplemented groups in contrast to the uracil supplemented group that decreased with the higher doses of the dietary supplementation and compared to the control.…”

“…It plays an important role in inflammation as it recognizes and binds to sialylated carbohydrates present on the surface proteins of leukocytes (Jubeli et al, 2012). In fish, the E‐selectin gene is involved in embryonic development and is important for haematopoiesis, and therefore, it has a biological role in fish similar to mammalian E‐selectins (Sun et al, 2015). In the present study, E‐selectine gene expressed in a dose‐dependent manner in inosine supplemented groups in contrast to the uracil supplemented group that decreased with the higher doses of the dietary supplementation and compared to the control.…”

“…In the present study, E‐selectine gene expressed in a dose‐dependent manner in inosine supplemented groups in contrast to the uracil supplemented group that decreased with the higher doses of the dietary supplementation and compared to the control. As mentioned, since E‐selectin is not constitutively expressed in endothelial cells (ECs), but its expression is induced by tumour necrosis factor‐α ( tnf ‐ α ) and, interleukin‐1β ( il ‐ 1β ) (Sun et al, 2015). This explained the dose‐dependent expression in sea bream fed the inosine supplemented group diets and confirmed the adverse effect on reduced its expression in the uracil supplemented groups suggests an indirect influence of nucleotides on expression of this gene.…”

Hemodynamic forces can be accurately measured in vivo with optical tweezers

“…Another important question that could be solved using this tool is the potential contribution of hemodynamics to intravascular arrest of immune or CTCs. Probing the shear stress in very close proximity to the vessel wall could help in understanding whether rolling of immune or tumor cells, usually attributed to selectin adhesion ( Brunk and Hammer, 1997 ; Lawrence et al , 1997 ; Sun et al , 2015 ), can be tuned by hemodynamics. During their journey in the blood circulation, CTCs are subjected to shear forces and collisions with host cells.…”

Hemodynamic forces can be accurately measuredin vivowith optical tweezers

“…Another important question that could be solved using this tool is the potential contribution of hemodynamics to intravascular arrest of immune or circulating tumor cells. Probing the shear stress in very close proximity to the vessel wall could help understanding whether rolling of immune or tumor cells, usually attributed to selectin adhesion (Brunk and Hammer, 1997;Lawrence et al, 1997;Sun et al, 2015), can be tuned by hemodynamics. During their journey in the blood circulation, CTCs are subjected to shear forces and collisions with host cells.…”