Identification and Expression Analysis of Chemosensory Genes in the Antennal Transcriptome of Chrysanthemum Aphid Macrosiphoniella sanborni

Abstract

As one of the most destructive oligophagous pests, the chrysanthemum aphid (Macrosiphoniella sanborni) has seriously restricted the sustainable development of the chrysanthemum industry. Olfaction plays a critical role in the environmental perception of aphids, but very little is currently known about the chemosensory mechanism of M. sanborni. In this study, four MsanOBPs, four MsanCSPs, eight MsanORs, two MsanIRs and one MsanSNMP were identified among the 28,323 unigenes derived from the antennal transcriptome bioinformatic analysis of M. sanborni adults. Then, comprehensive phylogenetic analyses of these olfactory-related proteins in different aphid species were performed using multiple sequence alignment. Subsequently, the odor-specific and wing-specific expression profiles of these candidate chemosensory genes were investigated using quantitative real-time PCR. The data showed that most of these chemosensory genes exhibited higher expression levels in alate aphids. Among them, MsanOBP9, MsanOR2, MsanOR4, MsanOR43b-1, MsanCSP1, MsanCSP2, MsanCSP4, MsanIR25a and MsanIR40a in alate aphids showed remarkably higher expression levels than in apterous aphids under the effect of the host plant volatiles, indicating that these genes may take part in the specific behaviors of alate adults, such as host recognition, oviposition site selection and so on. This study lays the groundwork for future research into the molecular mechanism of olfactory recognition in M. sanborni.

Perfect Complementarity Mechanism for Aphid Control: Oligonucleotide Insecticide Macsan-11 Selectively Causes High Mortality Rate for Macrosiphoniella sanborni Gillette

“…In addition, aphids provoke the development of sooty fungi [5]. All these factors deplete the plant, reduce the efficiency of photosynthesis, and disrupt metabolism, affecting the profitability of flower production [6] and subsequently leading to economic loss [7].…”

Candidate odorant‐binding protein and chemosensory protein genes in the turnip aphidLipaphis erysimi

“…In the present study, transcriptome sequencing provided a dataset of 11 LeryOBPs and four LeryCSPs . This comparatively smaller number of LeryOBP / LeryCSP genes can be compared to those of other reported aphid species, such as 13 OBPs and 5 CSPs in S. avenae (Xue et al, 2016), 4 OBPs and 4 CSPs in M. sanborni (Zhong et al, 2022), as well as 9 OBPs and 9 CSPs in M. persicae (Wang et al, 2019). Interestingly, the olfactory function of OBPs/CSPs seems to be conserved within aphids, reflected by the identified one‐to‐one orthologous relationship among the studied species.…”

“…CSPs in M. sanborni (Zhong et al, 2022), as well as 9 OBPs and 9 CSPs in M. persicae (Wang et al, 2019).…”

“…Antennal transcriptomes have been successfully used to identify insect chemosensory genes, even at low abundance (Cao et al, 2014; Cheng et al, 2019; Li et al, 2017; Liu et al, 2012; Wang et al, 2017, 2023b; Xing et al, 2021; Xue et al, 2016; Yuvaraj et al, 2018; Zhong et al, 2022). Examples include antennal transcriptomes that identified 26 OBPs and 12 CSPs in the bollworm Helicoverpa armigera (Liu et al, 2012), 46 OBPs and 16 CSPs in the citrus longhorned beetle Anoplophora chinensis (Wang et al, 2017), and 15 OBPs and 18 CSPs in the coneworm Dioryctria abietella (Xing et al, 2021).…”

“…Branch support was assessed by a bootstrap method based on 1000 replicates. The dataset submitted for phylogenetic analysis consisted of the annotated L. erysimi OBP and CSP genes identified in this study, as well as previously reported OBPs and CSPs from the pea aphid Acyrthosiphon pisum (Zhou et al, 2010), the cotton aphid A. gossypii (Gu et al, 2013), the green peach aphid Myzus persicae (Wang et al, 2019), the chrysanthemum aphid Macrosiphoniella sanborni (Zhong et al, 2022), and the grain aphid Sitobion avenae (Xue et al, 2016); further, the OBPs from the soybean aphid Aphis glycines (Wang et al, 2020) and the CSPs from the grape phylloxera Daktulosphaira vitifoliae (Zhao et al, 2017) were also submitted. Detailed sequence information is listed in Supporting Information Files S1 and S2.…”