Identification and expression analysis of an olfactory receptor gene family in green plant bug Apolygus lucorum (Meyer-Dür)

An, Xiaopeng; Sun, Liang; Liu, Hangwei; Liu, Danfeng; Ding, Yu-Xiao; Li, Le-Mei; Zhang, Yongjun; Guo, Yong

doi:10.1038/srep37870

Cited by 42 publications

(48 citation statements)

References 53 publications

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“…Second‐instar larvae were used for further experiments. Nymphs of A. lucorum feeding on green beans ( Phaseolus vulgaris ) were cultivated in climatic chambers at 29 ± 1 °C, 60 ± 5% RH and 14L : 10D photoperiod (An et al ., ). Three‐day‐old A. lucorum adults were employed for the following assays.…”

Section: Methodsmentioning

confidence: 97%

Identification and functional analysis of two P450 enzymes of Gossypium hirsutum involved in DMNT and TMTT biosynthesis

Liu

Huang

Jing

et al. 2017

Plant Biotechnology Journal

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SummaryThe homoterpenes (3E)‐4,8‐dimethyl‐1,3,7‐nonatriene (DMNT) and (E,E)‐4,8,12‐trimethyl‐1,3,7,11‐tridecatetraene (TMTT) are major herbivore‐induced plant volatiles that can attract predatory or parasitic arthropods to protect injured plants from herbivore attack. In this study, DMNT and TMTT were confirmed to be emitted from cotton (Gossypium hirsutum) plants infested with chewing caterpillars or sucking bugs. Two CYP genes (GhCYP82L1 and GhCYP82L2) involved in homoterpene biosynthesis in G. hirsutum were newly identified and characterized. Yeast recombinant expression and enzyme assays indicated that the two GhCYP82Ls are both responsible for the conversion of (E)‐nerolidol to DMNT and (E,E)‐geranyllinalool to TMTT. The two heterologously expressed proteins without cytochrome P450 reductase fail to convert the substrates to homoterpenes. Quantitative real‐time PCR (qPCR) analysis suggested that the two GhCYP82L genes were significantly up‐regulated in leaves and stems of G. hirsutum after herbivore attack. Subsequently, electroantennogram recordings showed that electroantennal responses of Microplitis mediator and Peristenus spretus to DMNT and TMTT were both dose dependent. Laboratory behavioural bioassays showed that females of both wasp species responded positively to DMNT and males and females of M. mediator could be attracted by TMTT. The results provide a better understanding of homoterpene biosynthesis in G. hirsutum and of the potential influence of homoterpenes on the behaviour of natural enemies, which lay a foundation to study genetically modified homoterpene biosynthesis and its possible application in agricultural pest control.

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Section: Methodsmentioning

confidence: 97%

Identification and functional analysis of two P450 enzymes of Gossypium hirsutum involved in DMNT and TMTT biosynthesis

Liu

Huang

Jing

et al. 2017

Plant Biotechnology Journal

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“…[3][4][5] A. lucorum, a notorious pest in China, has been intensively and systematically studied over the past two decades in regard to the regularity of outbreaks, its biology, behavior, molecular biology and control technologies. [6][7][8][9][10] Currently, the most effective and frequently used methods to control this plant bug in China involve the use of synthetic pesticides, 11 and new, more environmentally acceptable approaches are required to minimize or avoid use of these pesticides. Pheromones could provide such approaches for monitoring and controlling pests.…”

Section: Introductionmentioning

confidence: 99%

Identification and field evaluation of the sex pheromone of Apolygus lucorum (Hemiptera: Miridae) in China

Tao

Mei

Zhang

et al. 2020

Pest Management Science

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BACKGROUND The plant bug, Apolygus lucorum Meyer‐Dür, has begun a resurgence and has become a key pest in cotton in northern China, with the wide‐scale adoption of transgenic Bt cotton. We attempted to develop a new approach to the control of this plant bug by identifying and utilizing its sex pheromone. RESULTS Extracts from A. lucorum adults and nymphs were analyzed using gas chromatography–mass spectrometry (GC–MS) and gas chromatography–electroantennogram detection (GC–EAD) to identify the sex pheromone components. (E)‐4‐Oxo‐2‐hexenal and (E)‐2‐hexenyl butyrate were the major sex pheromone components from females and were also electrophysiology‐active towards male antennae. For males, (E)‐4‐oxo‐2‐hexenal and hexyl butyrate were the major sex pheromone components. These three components, however, were not detected in nymphs. Field tests showed that binary blends of (E)‐4‐oxo‐2‐hexenal and (E)‐2‐hexenyl butyrate at a ratio of 3:2 resulted in trapping the greatest numbers of A. lucorum males. A long‐term dispenser was developed by loading the pheromone components dissolved in sunflower oil into polyethylene vials. Further field evaluation showed that the polyethylene dispensers attracted significantly more bugs even when deployed in the field for 5 weeks. CONCLUSION We identified the sex pheromone of A. lucorum and developed a type of high‐efficiency and long‐term lure. Our results could expand knowledge of the pheromones of plant bugs, and provide novel technologies to monitor and control this pest. © 2019 Society of Chemical Industry

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“…papillosa antennal transcriptomes, a total of 59 candidate OR proteins were identified. This total number is much lower when compared with 110 antennal ORs reported in the green plant bug, Apolygus lucorum 42 . It is very likely that not all OR genes have been identified in this research.…”

Section: Discussionmentioning

confidence: 65%

Transcriptome sequencing of Tessaratoma papillosa antennae to identify and analyze expression patterns of putative olfaction genes

et al. 2017

Sci Rep

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Studies on insect olfaction have increased our understanding of insect’s chemosensory system and chemical ecology, and have improved pest control strategies based on insect behavior. In this study, we assembled the antennal transcriptomes of the lychee giant stink bug, Tessaratoma papillosa, by using next generation sequencing to identify the major olfaction gene families in this species. In total, 59 odorant receptors, 14 ionotropic receptors (8 antennal IRs), and 33 odorant binding proteins (28 classic OBPs and 5 plus-C OBPs) were identified from the male and female antennal transcriptomes. Analyses of tissue expression profiles revealed that all 59 OR transcripts, 2 of the 8 antennal IRs, and 6 of the 33 OBPs were primarily expressed in the antennae, suggesting their putative role in olfaction. The sex-biased expression patterns of these antenna-predominant genes suggested that they may have important functions in the reproductive behavior of these insects. This is the first report that provides a comprehensive resource to future studies on olfaction in the lychee giant stink bug.

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Identification and expression analysis of an olfactory receptor gene family in green plant bug Apolygus lucorum (Meyer-Dür)

Cited by 42 publications

References 53 publications

Identification and functional analysis of two P450 enzymes of Gossypium hirsutum involved in DMNT and TMTT biosynthesis

Identification and functional analysis of two P450 enzymes of Gossypium hirsutum involved in DMNT and TMTT biosynthesis

Identification and field evaluation of the sex pheromone of Apolygus lucorum (Hemiptera: Miridae) in China

Transcriptome sequencing of Tessaratoma papillosa antennae to identify and analyze expression patterns of putative olfaction genes

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