Identification and expression analysis of pineapple sugar transporters reveal their role in the development and environmental response

Fakher, Beenish; Jakada, Bello Hassan; Greaves, Joseph G.; Wang, Lulu; Niu, Xiaoping; Cheng, Yan; Zheng, Ping; Aslam, Mohammad; Qin, Yuan; Wang, Xiaomei

doi:10.3389/fpls.2022.964897

Cited by 9 publications

(3 citation statements)

References 93 publications

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“…It suggests that the low number of TMDs in some MSTs and SUTs may be due to incomplete transcriptome data ( Table S4 ). However, a low number of TMDs still possibly exists as reported in [ 53 , 59 ]. It is impossible to check all the incomplete sequences experimentally in the current study.…”

Section: Discussionmentioning

confidence: 99%

Identification and Expression Analysis of Putative Sugar Transporter Gene Family during Bulb Formation in Lilies

Huang,

Gao,

et al. 2024

IJMS

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Sugar transporters play important roles in plant growth and development, flowering and fruiting, as well as responses to adverse abiotic and biotic environmental conditions. Lilies (Lilium spp.) are some of the most representative ornamental bulbous flowers. Sugar metabolism is critical for bulb formation in lilies; therefore, clarifying the amount and expression pattern of sugar transporters is essential for further analyzing their roles in bulb formation. In this study, based on the transcriptome data of the Lilium Oriental hybrid ‘Sorbonne’ and Lilium × formolongi, a total of 69 and 41 sugar transporters were identified in ‘Sorbonne’ and Lilium × formolongi, respectively, by performing bioinformatics analysis. Through phylogenetic analysis, monosaccharide transporters (MSTs) can be divided into seven subfamilies, sucrose transporters (SUTs) can be divided into three subgroups, and sugars will eventually be exported transporters (SWEETs) can be divided into four clades. According to an analysis of conserved motifs, 20, 14, and 12 conserved motifs were predicted in MSTs, SUTs, and SWEETs, respectively. A conserved domain analysis showed that MSTs and SUTs contained a single domain, whereas most of the SWEETs harbored two MtN3/saliva domains, also known as a PQ-loop repeat. The LohINT1, which was predicted to have a smaller number of transmembrane structural domains, was cloned and analyzed for subcellular localization. It was found that the LohINT1 protein is mainly localized in the cell membrane. In addition, the expression analysis indicated that 22 LohMSTs, 1 LohSUTs, and 5 LohSWEETs were upregulated in ‘Sorbonne’ 1 day after scale detachment treatment, suggesting that they may regulate the initiation of the bulblet. A total of 10 LflMSTs, 1 LflSUTs, and 6 LflSWEETs were upregulated 4~6 months after sowing, which corresponds to the juvenile-to-adult transition phase of Lilium × formolongi, suggesting that they may also play a role in the accompanying bulb swelling process. Combined with quantitative real-time PCR (qRT-PCR) analysis, LohSTP8 and LohSTP12 were significantly overexpressed during the extremely early stage of bulblet initiation, and LflERD6.3 was significantly overexpressed during the growth of the underground bulblet, suggesting that they may be key sugar transporters in the formation of lily bulbs, which needs further functional verification.

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Section: Discussionmentioning

confidence: 99%

Identification and Expression Analysis of Putative Sugar Transporter Gene Family during Bulb Formation in Lilies

Huang,

Gao,

et al. 2024

IJMS

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“…For the yeast transport assays, the Saccharomyces cerevisiae strain EBY.VW4000 (Wieczorke et al, 1999) was used as described previously (Fakher et al, 2022). The ORFs of AcSWEET6, AcSWEET8, and AcSWEET10 were amplified using specific primer combinations.…”

Section: Glucose Transport Assay In Yeastmentioning

confidence: 99%

Substrate specificity and functional conservation of SWEET10 transporter in pineapple

Fakher

Ashraf

Wang

et al. 2023

Preprint

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SWEET transporters are a unique class of sugar transporters that play vital roles in various developmental and physiological processes in plants. While the functions of SWEETs have been well established in model plants such as Arabidopsis, their functions in economically important fruit crops like pineapple have not been well studied. Here, we aimed to investigate the substrate specificity of pineapple SWEETs by comparing the protein sequences of known glucose and sucrose transporters in Arabidopsis to those in pineapple. Our genome-wide approach and 3D structure comparison showed that the Arabidopsis SWEET8 homolog in pineapple, AcSWEET10, shares similar sequences and protein properties responsible for glucose transport. To determine the functional conservation of AcSWEET10, we tested its ability to complement glucose transport mutants in yeast and analyzed its expression in stamens and impact on the microspore phenotype and seed set in transgenic Arabidopsis. The results showed that AcSWEET10 is functionally equivalent to AtSWEET8 and plays a critical role in regulating microspore formation through the regulation of the Callose synthase5 (CalS5), which highlights the importance of SWEET transporters in pineapple. This information could have important implications for improving fruit crop yield and quality by manipulating SWEET transporter activity.

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“…SUTs have also been identified in other species. A total of 49 sugar transporter gene sequences belonging to MST (45) and SUC/SUT (4) were identified in the pineapple genome [14]. A total of nine SUTs were identified in the beet genome and divided into groups 1, 2, and 3, which were unevenly distributed on four chromosomes [15].…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification and Expression Analysis of the SUT Family from Three Species of Sapindaceae Revealed Their Role in the Accumulation of Sugars in Fruits

Jiang,

An,

Xia

et al. 2023

Plants

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Sapindaceae is an economically important family of Sapindales and includes many fruit crops. The dominant transport and storage form of photoassimilates in higher plants is sucrose. Sucrose transporter proteins play an irreplaceable role in the loading, transportation, unloading, and distribution of sucrose. A few SUT (sugar transporter) family genes have been identified and characterized in various plant species. In this study, 15, 15, and 10 genes were identified in litchi, longan, and rambutan, respectively, via genome-wide screening. These genes were divided into four subgroups based on phylogenetics. Gene duplication analysis suggested these genes underwent potent purifying selection and tandem duplications during evolution. The expression levels of SlSut01 and SlSut08 were significantly increased in the fruits of Sapindaceae members. The homologs of these two genes in longan and rambutan were also highly expressed in the fruits. The expression pattern of SUTs in three organs of the two varieties was also explored. Subcellular colocalization experiments revealed that the proteins encoded by both genes were present in the plasma membrane. This report provides data for the functional study of SUTs in litchi and provides a basis for screening sugar accumulation-related genes in fruits of Sapindaceae.

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Identification and expression analysis of pineapple sugar transporters reveal their role in the development and environmental response

Cited by 9 publications

References 93 publications

Identification and Expression Analysis of Putative Sugar Transporter Gene Family during Bulb Formation in Lilies

Identification and Expression Analysis of Putative Sugar Transporter Gene Family during Bulb Formation in Lilies

Substrate specificity and functional conservation of SWEET10 transporter in pineapple

Genome-Wide Identification and Expression Analysis of the SUT Family from Three Species of Sapindaceae Revealed Their Role in the Accumulation of Sugars in Fruits

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