Identification and expression analysis of G protein-coupled receptors in the cotton aphid, Aphis gossypii Glover

Li, Jing; Lv, Haixiang; Li, Xuchao; Yao, Yongsheng; Li, Jianhong; Ma, Kangsheng

doi:10.1016/j.ijbiomac.2022.10.108

Cited by 8 publications

(8 citation statements)

References 67 publications

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“…49 The high expression of most GPCR genes in the head implies a neuromodulatory role in insect physiological processes including reproduction, growth, development, behavior, and vision. 27 Similar results have been found in other species. 47 This study focused on genes that exhibit high expression in the midgut, which plays a crucial role in detoxification and metabolism.…”

Section: Discussionsupporting

confidence: 83%

“…53 Aphis gossypii, on the other hand, exhibited the induction of 10 GPCRs upon exposure to LC 50 of sulfoxaflor, and two of these were found to be associated with sulfoxaflor resistance through RNAi. 27 However, there is a dearth of studies investigating the extent to which upregulated GPCR genes contribute to insecticide treatment across different insect species, necessitating further research. The aforementioned studies suggest that the upregulation of GPCR genes following insecticide treatment may be linked to detoxification, and additional functional investigations are warranted.…”

Section: Discussionmentioning

confidence: 99%

“…The conserved protein domains of GPCR genes were predicted using the NCBI Conserved Domain Database (CDD) (). GPCR candidates with less than 4 7TM structural domains or incorrect annotation information were excluded based on the predicted motifs from the resultant domains . The duplication types of the different GPCR genes were classified using the MCScanX; visual analysis of the locations of all GPCR genes and gene tandem duplication events was accomplished using TBtools …”

Section: Methodsmentioning

confidence: 99%

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Identification of G Protein-Coupled Receptors (GPCRs) Associated with Lambda-Cyhalothrin Detoxification in Cydia pomonella

Liu,

Hu,

et al. 2023

J. Agric. Food Chem.

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While previous studies have reported G protein-coupled receptor (GPCR)-mediated insecticide resistance in various arthropods, the understanding of GPCR-associated resistance mechanisms in Cydia pomonella remains limited. In this study, a total of 95 CpGPCR genes categorized into four families were identified in C. pomonella. Results revealed high expression levels of the majority of the CpGPCRs during the first larval stage and in the head of C. pomonella. Exposure to lambda-cyhalothrin significantly increased the expression of 15 CpGPCRs, including CpGPCR70, which is highly expressed in all larval stages and shows the highest expression in the midgut. RNA interference (RNAi) demonstrated that downregulation of CpGPCR70 leads to reduced expression of key resistance-related genes and a decreased tolerance of larvae to lambda-cyhalothrin. These findings indicate that CpGPCR70 plays a crucial role in regulating the expression of detoxifying genes involved in lambda-cyhalothrin resistance, offering valuable insights for the development of more effective pest control strategies.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Identification of G Protein-Coupled Receptors (GPCRs) Associated with Lambda-Cyhalothrin Detoxification in Cydia pomonella

Liu,

Hu,

et al. 2023

J. Agric. Food Chem.

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“…Another study found a relationship between the enhancement of P450 activity and overexpression of P450 genes with sulfoxaflor resistance in A. gossypii , with the authors noting the importance of the CYP6CY13–2 gene 48 . Contrarily, a later study suggested the overexpression of G protein‐coupled receptor genes likely contributed to sulfoxaflor resistance in A. gossypii , 51 while another study involving A. gossypii provided evidence that the overexpression of UDP‐glycosyltransferases from the UGT344 family contributes to sulfoxaflor resistance 52 . Yet another study suggested that the overexpression of ATP‐binding cassette transporters, particularly ABCA1 and ABCD1 genes, may be related to sulfoxaflor resistance in A. gossypii 53 .…”

Section: Discussionmentioning

confidence: 98%

The evolving story of sulfoxaflor resistance in the green peach aphid,Myzus persicae(Sulzer)

Ward,

Jalali,

van Rooyen

et al. 2023

Pest Management Science

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BACKGROUNDThe green peach aphid, Myzus persicae (Sulzer), is one of the most economically important crop pests worldwide. Insecticide resistance in this pest was first detected over 60 years ago, with resistance in M. persicae now spanning over 80 active ingredients. Sulfoxaflor, is a relatively new insecticide that is primarily used to control sap‐feeding insects. In 2018, resistance to sulfoxaflor was discovered in field populations of M. persicae in Australia. This study aimed to determine the current distribution and phenotypic levels of sulfoxaflor resistance in Australian clones of M. persicae and to investigate how these patterns relate to clonal type.RESULTSFor the first time, we show there is low‐level resistance (8‐26‐fold) distributed across Australia, with resistance being detected in aphids collected from approximately 20% of all M. persicae collected and screened. Furthermore, although previously thought to exist in only a single haplotype, this study discovered sulfoxaflor resistance in a second M. persicae haplotype, providing evidence that there have been multiple independent evolutionary events which have given rise to sulfoxaflor resistance in this species.CONCLUSIONThese findings have important implications for the chemical control of M. persicae in Australia, especially when considering the broader genetic background of these aphids which are known to harbour a number of other insecticide resistance mechanisms. We recommend continuous monitoring of sulfoxaflor resistance in field populations of M. persicae (in Australia and elsewhere) and further research into the underlying genetic mechanisms conferring resistance to sulfoxaflor in both clonal haplotypes.This article is protected by copyright. All rights reserved.

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“…While numerous studies have documented transcriptional responses of P450s to xenobiotics, including phytochemicals and insecticides, the mechanisms underlying the regulation of enhanced enzymatic detoxification are still poorly understood. ,, Several signaling pathways involved in xenobiotic response have been identified by recent studies using advanced genetic tools, and upregulation or activation of some components of these pathways has been linked to the transcriptional regulation of detoxification genes. ,− Among them, CncC and its partner Maf have been implicated in the regulation of a battery of detoxification genes associated with insecticide resistance, as well as phytochemical adaptation. − In this study, CncC and MafK were significantly induced by flavone, xanthotoxin, coumarin, and λ-cyhalothrin, which is in accordance with the induced expression patterns of the CYP321A subfamily genes. In view of our recent findings that CncC regulates 80% of the detoxification genes induced by flavone and upregulation of CYP6AB12 conferring tolerance of S.…”

Section: Discussionmentioning

confidence: 99%

CYP321A Subfamily P450s Contribute to the Detoxification of Phytochemicals and Pyrethroids in Spodoptera litura

Xiao,

Wang,

Deng

et al. 2023

J. Agric. Food Chem.

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Although the induction of cytochrome P450 monooxygenases involved in insect detoxification has been well documented, the underlying regulatory mechanisms remain obscure. In Spodoptera litura, CYP321A subfamily members were effectively induced by exposure to flavone, xanthotoxin, curcumin, and λ-cyhalothrin, while knockdown of the CYP321A genes increased larval susceptibility to these xenobiotics. Homology modeling and molecular docking analyses showed that these four xenobiotics could stably bind to the CYP321A enzymes. Furthermore, two transcription factor genes, CncC and Maf K, were significantly induced by the xenobiotics. Knockdown of CncC or Maf K reduced the expression of four CYP321A genes and increased larval susceptibility to the xenobiotics. Dual-luciferase reporter assays showed that cotransfection of reporter plasmids carrying the CYP321A promoter with CncC and/or MafK-expressing constructs significantly magnified the promoter activity. These results indicate that the induction of CYP321A subfamily members conferring larval detoxification capability to xenobiotics is mediated by the activation of CncC and MafK.

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Identification and expression analysis of G protein-coupled receptors in the cotton aphid, Aphis gossypii Glover

Cited by 8 publications

References 67 publications

Identification of G Protein-Coupled Receptors (GPCRs) Associated with Lambda-Cyhalothrin Detoxification in Cydia pomonella

Identification of G Protein-Coupled Receptors (GPCRs) Associated with Lambda-Cyhalothrin Detoxification in Cydia pomonella

The evolving story of sulfoxaflor resistance in the green peach aphid,Myzus persicae(Sulzer)

CYP321A Subfamily P450s Contribute to the Detoxification of Phytochemicals and Pyrethroids in Spodoptera litura

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