Identification and expression of immune genes in the flat oyster Ostrea edulis in response to bonamiosis

Martín-Gómez, Laura; Villalba, António; Abollo, Elvira

doi:10.1016/j.gene.2011.11.001

Cited by 38 publications

(22 citation statements)

References 63 publications

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“…The role of a number of lysosomal enzymes, anti protease activity in the host serum and levels of expression of heat shock proteins in the host haemolymph has been analysed and the results are being processed for publication. Recent genomic (Morga et al, 2011b;Martín-Gómez et al, 2012) and proteomic (Cao et al, 2009) approaches could help to disclose genes and biochemical processes responsible for bonamiosis resistance. Availability of molecular diagnostic tools allowed to detect B. ostreae in early stages of oyster development (Lynch et al, 2005;Arzul et al, 2011) but infection uses to reach clinical levels in adult oysters.…”

Section: Discussionmentioning

confidence: 99%

Comparison of haemocytic parameters among flat oyster Ostrea edulis stocks with different susceptibility to bonamiosis and the Pacific oyster Crassostrea gigas

Comesaña

Casas

Cao

et al. 2012

Journal of Invertebrate Pathology

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Farming of the flat oyster Ostrea edulis in Europe is severely constrained by the protozoan Bonamia ostreae. The introduction of the resistant species Crassostrea gigas has been a relief for the farmers, while the pilot programmes to select O. edulis strains resistant to bonamiosis performed in various countries can be seen as a promising strategy to minimise the effects of bonamiosis. However, the physiological bases of this differential susceptibility remain unknown. A search for an explanation of the intra and interspecific differences in oyster susceptibility to bonamiosis was accomplished by comparing some immune parameters among various O. edulis stocks and C. gigas. On December 2003, naïve and Bonamia-relatively resistant flat oysters from Ireland, Galician flat oysters and Pacific oysters C. gigas were deployed in a Galician area affected by bonamiosis; haemolymph samples were taken in February and May 2004. A new oyster deployment at the same place was carried out on June 2004 and haemolymph sampling was performed on April 2005. On November 2004, new sets of Irish flat oysters and C. gigas were deployed in Ireland and haemolymph sampling was performed in June 2005. Various haemocytic parameters were measured: total and differential haemocyte count, phagocytic ability, respiratory burst (superoxide anion [O(2)(-)] and hydrogen peroxide [H(2)O(2)]) and nitric oxide [NO] production. The comparison of the parameters was carried out at 3 levels: (1) between O. edulis and C. gigas, (2) among O. edulis stocks with different susceptibility to bonamiosis, and (3) between Bonamia-infected and non infected O. edulis. In addition, haemocyte-B. ostreaein vitro encounters were performed to analyse interspecific differences in the haemocytic respiratory burst, using flow cytometry. Significant differences associated with total and differential haemocyte count, and respiratory burst between O. edulis and C. gigas were detected, which could be linked to differences in susceptibility to bonamiosis between both species. Additionally, significant changes in total and differential haemocyte count, and respiratory burst of O. edulis associated with B. ostreae infection were found. However, no consistent difference in any haemocyte parameter between the O. edulis stocks involved in the study was recorded.

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Section: Discussionmentioning

confidence: 99%

Comparison of haemocytic parameters among flat oyster Ostrea edulis stocks with different susceptibility to bonamiosis and the Pacific oyster Crassostrea gigas

Comesaña

Casas

Cao

et al. 2012

Journal of Invertebrate Pathology

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“…At a molecular level, B. ostreae modifies the expression pattern of immune related genes such as galectin, superoxide dismutase (SOD) and filamin [33,34].…”

Section: Introductionmentioning

confidence: 99%

Oral immunostimulation of the oyster Ostrea edulis: Impacts on the parasite Bonamia ostreae

Prado-Álvarez

Lynch

Kane

et al. 2015

Fish & Shellfish Immunology

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“…This gene appeared to be over-expressed in haemocytes within 1 h of contact with the parasites, suggesting that the Oe-Gal is involved in the recognition of the parasite (Morga et al 2011). Moreover, the cytoskeleton of the host cell seems to be involved in the internalization of the para site as suggested by the over-expression of a gene coding a filamin in infected haemocytes (Morga et al 2011) and the abundance of some genes related to the cytoskeleton in the libraries obtained from infected flat oysters (Martín-Gómez et al 2012).…”

Section: Host−parasite Interactionsmentioning

confidence: 99%

“…Several genes encoding proteins in volved in detoxication and degradation including cytosolic SOD, omega glutathione Stransferase, tissue inhibitors of metalloproteinase and an interferon regulatory like factor were overexpressed in haemocytes in contact with live parasites (Morga et al 2011). The energetic reserves of the host appear mobilized to obtain the energy to counter the parasite as demonstrated by the up-regulation of genes involved in metabolism, such as immunoresponsive gene 1 (Martín-Gómez et al 2012). A significant reduction of phenoloxidase activity in the haemo lymph of flat oysters infected with the parasite was also reported in comparison with uninfected oysters (da Silva et al 2008).…”

Section: Host−parasite Interactionsmentioning

confidence: 99%

“…Recent studies have focused on gaining a greater understanding of the response of oysters to B. ostreae infection (Morga et al 2009, 2011, 2012, Comesaña et al 2012, Martín-Gómez et al 2012. Functional studies combining more sophisticated genetic methodology, proteomics and cellular and ultrastructural approaches will be required to better characterize how these mechanisms allow an oyster host to resist Bonamia infection and the parasite to escape host responses.…”

Section: Future Directionsmentioning

confidence: 99%

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Bonamia parasites: a rapidly changing perspective on a genus of important mollusc pathogens

Engelsma¹,

Culloty²,

Lynch³

et al. 2014

Dis. Aquat. Org.

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Organisms of the genus Bonamia are intracellular protistan parasites of oysters. To date, 4 species have been described (B. ostreae, B. exitiosa, B. perspora and B. roughleyi), al though the status of B. roughleyi is controversial. Introduction especially of B. ostreae and B. exitiosa to naïve host populations has been shown to cause mass mortalities in the past and has had a dramatic impact on oyster production. Both B. ostreae and B. exitiosa are pathogens notifiable to the World Organisation for Animal Health (OIE) and the European Union. Effective management of the disease caused by these pathogens is complicated by the extensive nature of the oyster production process and limited options for disease control of the cultured stocks in open water. This review focuses on the recent advances in research on genetic relationships between Bonamia isolates, geographical distribution, susceptible host species, diagnostics, epizootiology, host− parasite interactions, and disease resistance and control of this globally important genus of oyster pathogens.KEY WORDS: Bonamia ostreae · Bonamia exitiosa · Oyster disease · Phylogenetics · Geographical spread · Diagnostics · Epizootiology · Host−parasite interaction · Bonamiosis Resale or republication not permitted without written consent of the publisher Contribution to DAO Special 7 'Microcell parasites of molluscs'

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Identification and expression of immune genes in the flat oyster Ostrea edulis in response to bonamiosis

Cited by 38 publications

References 63 publications

Comparison of haemocytic parameters among flat oyster Ostrea edulis stocks with different susceptibility to bonamiosis and the Pacific oyster Crassostrea gigas

Comparison of haemocytic parameters among flat oyster Ostrea edulis stocks with different susceptibility to bonamiosis and the Pacific oyster Crassostrea gigas

Oral immunostimulation of the oyster Ostrea edulis: Impacts on the parasite Bonamia ostreae

Bonamia parasites: a rapidly changing perspective on a genus of important mollusc pathogens

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