Identification and expression of the gene product encoding a CPD photolyase from Dunaliella salina

Cheng, Long; Qiao, Dai Rong; Xue, Lingxiao; Xiong, Yan; Bai, Lin; Xu, Hui; Yang, Yang; Cao, Yi

doi:10.1016/j.jphotobiol.2007.01.002

Cited by 10 publications

(7 citation statements)

References 31 publications

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“…34 The PL-mediated photoreactivation process has been widely demonstrated in phytoplankton. 35,36 Cheng et al (2007) 37 isolated the gene encoding a CPD-PL from D. salina, which was induced by UVR. Recently, two members of the cryptochrome/photolyase family have been characterized in the prasinophyte Ostreococcus taurii displaying photolyase activity as well.…”

Section: Introductionmentioning

confidence: 99%

Elevated CO2 alleviates high PAR and UV stress in the unicellular chlorophyte Dunaliella tertiolecta

García‐Gómez

Gordillo

Palma

et al. 2014

Photochem Photobiol Sci

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The effects of increased CO₂ and irradiance on the physiological performance of the chlorophyte Dunaliella tertiolecta were studied at different PAR and UVR (UVA+UVB) irradiances, simulating the solar radiation at different depths, under present (390 ppmv, LC) and predicted CO₂ levels (1000 ppmv, HC). Cell stress after UVR-exposure was mostly attenuated under HC levels, as evidenced by a decrease in reactive oxygen species accumulation. DNA damage showed a 42-fold increase in cyclobutane-pyrimidine dimers formation under the highest irradiance in LC with respect to the lowest irradiance. Photolyase gene expression was upregulated under HC resulting in a drastic decrease in CPDs accumulation to only 25% with respect to LC. However, the expression of genes related to the replacement of photosynthetic apparatus proteins (PsbA and LHCII) were downregulated at HC compared to LC. Proliferating cell nuclear antigen (PCNA) accumulation was always higher in HC and the accumulation pattern indicated its involvement in DNA repair or growth depending on the irradiance doses. Our results suggest that marine unicellular chlorophytes might possibly become more resilient to UVR exposure under future CO₂ regimes.

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Section: Introductionmentioning

confidence: 99%

Elevated CO2 alleviates high PAR and UV stress in the unicellular chlorophyte Dunaliella tertiolecta

García‐Gómez

Gordillo

Palma

et al. 2014

Photochem Photobiol Sci

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“…faecalis as in D. salina by leading to DNA double‐strand breaks (by NaCl) and DNA oxidative damage (by H 2 O 2 ) (Cheng et al . ). It is possible that more repaired CPDs by photolyase in light under salt stress resulted in the increase in bacteria concentrations.…”

Section: Discussionmentioning

confidence: 97%

“…faecalis and Dunaliella salina are salt tolerant (Ramos et al 2011;Byappanahalli et al 2012), high salinity may cause the expression of CPD photolyase in Ent. faecalis as in D. salina by leading to DNA doublestrand breaks (by NaCl) and DNA oxidative damage (by H 2 O 2 ) (Cheng et al 2007). It is possible that more repaired CPDs by photolyase in light under salt stress resulted in the increase in bacteria concentrations.…”

Section: Discussionmentioning

confidence: 99%

Effects of salinity and temperature on inactivation and repair potential of Enterococcus faecalis following medium- and low-pressure ultraviolet irradiation

et al. 2016

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“…As in photosynthesis, CPDs are predominantly induced when the cells are close to the surface and repaired when near the thermocline in the UML (Helbling et al, 2008). In addition to excision repair, mismatch repair and SOS response, mainly the photoactivated CPD photolyase is engaged to break the dimers using the energy of UV-A or blue light photons (Cheng et al, 2007;Ioki et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Interactions of anthropogenic stress factors on marine phytoplankton

Häder¹,

Gao

2015

Front. Environ. Sci.

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Phytoplankton are the main primary producers in aquatic ecosystems. Their biomass production and CO 2 sequestration equals that of all terrestrial plants taken together. Phytoplankton productivity is controlled by a number of environmental factors, many of which currently undergo substantial changes due to anthropogenic global climate change. Most of these factors interact either additively or synergistically. Light availability is an absolute requirement for photosynthesis, but excessive visible and UV radiation impair productivity. Increasing temperatures enhance stratification and decrease the depth of the upper mixing layer exposing the cells to higher solar radiation and reduce nutrient upward transport from upwelling deeper water. At the same time, stratospheric ozone depletion exposes phytoplankton to higher solar UV-B radiation especially in polar and mid-latitudes. Terrestrial runoff carrying sediments and dissolved organic matter into coastal waters leads to eutrophication while reducing UV penetration. All these environmental forcings are known to affect physiological and ecological processes. Ocean acidification due to increased atmospheric CO 2 concentrations changes the seawater chemistry; it reduces calcification in phytoplankton, macroalgae and many zoological taxa. Ocean warming results in changing species composition and favors blooms of toxic prokaryotic and eukaryotic phytoplankton. Increasing pollution from crude oil spills, persistent organic pollutants, heavy metal as well as industrial and household wastewaters affect phytoplankton which is augmented by solar UV radiation. Extensive analyses of the impacts of multiple stressors are scarce. Here, we review reported findings on the impacts of anthropogenic stressors on phytoplankton with an emphasis on their interactive effects and make an effort to provide a prospect for future studies.

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Identification and expression of the gene product encoding a CPD photolyase from Dunaliella salina

Cited by 10 publications

References 31 publications

Elevated CO2 alleviates high PAR and UV stress in the unicellular chlorophyte Dunaliella tertiolecta

Elevated CO2 alleviates high PAR and UV stress in the unicellular chlorophyte Dunaliella tertiolecta

Effects of salinity and temperature on inactivation and repair potential of Enterococcus faecalis following medium- and low-pressure ultraviolet irradiation

Interactions of anthropogenic stress factors on marine phytoplankton

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