Identification and expression patterns of alcohol dehydrogenase genes involving in ester volatile biosynthesis in pear fruit

Qin, Gaihua; Qi, Xiaoxiao; Qi, Yingjie; Gao, Zeng Liang; Yi, Xingkai; Pan, Haifa; Xu, Yongsheng

doi:10.1016/s2095-3119(17)61686-1

Cited by 19 publications

(13 citation statements)

References 33 publications

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“…Among the ADH and TPS gene families, the expression levels of PeADH13/26/33 and PeTPS2/3/4/24 increased gradually with fruit development, which is consistent with the increasing trend of ester and terpene metabolite contents. The same results were reported in studies of mango 66,67 and pear 68 . These results indicated that these genes may play an important role in passion fruit flavor biosynthesis.…”

Section: Discussionsupporting

confidence: 87%

Chromosome-scale genome assembly provides insights into the evolution and flavor synthesis of passion fruit (Passiflora edulis Sims)

et al. 2021

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Passion fruit (Passiflora edulis Sims) is an economically valuable fruit that is cultivated in tropical and subtropical regions of the world. Here, we report an ~1341.7 Mb chromosome-scale genome assembly of passion fruit, with 98.91% (~1327.18 Mb) of the assembly assigned to nine pseudochromosomes. The genome includes 23,171 protein-coding genes, and most of the assembled sequences are repetitive sequences, with long-terminal repeats (LTRs) being the most abundant. Phylogenetic analysis revealed that passion fruit diverged after Brassicaceae and before Euphorbiaceae. Ks analysis showed that two whole-genome duplication events occurred in passion fruit at 65 MYA and 12 MYA, which may have contributed to its large genome size. An integrated analysis of genomic, transcriptomic, and metabolomic data showed that ‘alpha-linolenic acid metabolism’, ‘metabolic pathways’, and ‘secondary metabolic pathways’ were the main pathways involved in the synthesis of important volatile organic compounds (VOCs) in passion fruit, and this analysis identified some candidate genes, including GDP-fucose Transporter 1-like, Tetratricopeptide repeat protein 33, protein NETWORKED 4B isoform X1, and Golgin Subfamily A member 6-like protein 22. In addition, we identified 13 important gene families in fatty acid pathways and eight important gene families in terpene pathways. Gene family analysis showed that the ACX, ADH, ALDH, and HPL gene families, especially ACX13/14/15/20, ADH13/26/33, ALDH1/4/21, and HPL4/6, were the key genes for ester synthesis, while the TPS gene family, especially PeTPS2/3/4/24, was the key gene family for terpene synthesis. This work provides insights into genome evolution and flavor trait biology and offers valuable resources for the improved cultivation of passion fruit.

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Section: Discussionsupporting

confidence: 87%

Chromosome-scale genome assembly provides insights into the evolution and flavor synthesis of passion fruit (Passiflora edulis Sims)

et al. 2021

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“…1A). This was similar to the structure of pear PbrADHs reported by Qin et al [8]. ADH is a member of the medium-length dehydrogenase/reductase (MDR) protein superfamily.…”

Section: Structural Characterization Of Taadhssupporting

confidence: 89%

“…With the development of sequencing technology, the genome sequences of many species can be analyzed, which promotes the identi cation of plant gene families at the genome-wide level. The ADH genes have been detected in various plants, including tomato [5], rice [21], barley [22], melon [7], and pear [8]. With the publication of the wheat genome, it is possible to systematically study the function of wheat TaADH family members.…”

Section: Discussionmentioning

confidence: 99%

“…Most plant ADHs are dependent on Zn-MDRs. There are many reports about the genes of this family in recent years, and some studies mainly focus on the relationship between ADHs and aroma synthesis during fruit development [5][6][7][8], these are only part of its function, while the other functions of ADHs remain to be explained.…”

Section: Introductionmentioning

confidence: 99%

“…By analyzing the promoter elements of these genes, it is found that CmADHs is involved in the response of hormones such as ABA, IAA, and ethylene. Qin et al [8] found eight PbrADH genes in the pear genome with intron 8-9. These Pbr-ADH proteins have many conserved domains, such as the GroES-like domain (35-164 amino acids) and the zinc-binding domain (206-340 amino acids), which belong to the typical ADH family.…”

Section: Introductionmentioning

confidence: 99%

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Alcohol dehydrogenase (ADH) genes family in wheat (Triticum aestivum): Genome-wide identification, characterization, phylogenetic relationship and expression patterns

Shen

Yuan

2020

Preprint

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Background Alcohol dehydrogenase (ADH) plays important roles in plant survival under anaerobic conditions. Although some research has been carried out the functions of ADH in other plants, that of wheat TaADH family genes in response to abiotic stress are unclear. Results A total of 22 ADH genes were obtained from 14 chromosomes of the wheat genome by systematic screening. Multiple sequence alignment and evolutionary relationship show that these genes contain the characteristics of GroES-like domain and Zinc-binding domain, and these belong to Medium-chain -ADH type and can be divided into three subfamilies. There are 17 pairs of fragment replication genes among TaADH family members in the wheat genome, while there are 9 pairs of collinear gene pairs from ADH family members between wheat and rice genome. We speculate that these fragment repetition events may be the main reason for the amplification of TaADH family genes. Ka/Ks analysis indicated that there were 64 repetitive gene pairs, and the Ka/Ks value of these gene pairs was less than 1, which indicated that these sequences of TaADH gene were relatively conservative and did not change greatly in the process of evolution. Promoter element analysis showed that almost all of the upstream promoters of these genes contained the responsive anaerobic inducible element. Tissue localized expression and expression patterns also demonstrated that the TaADH genes responded to abiotic stress and may play an important role in waterlogging stress during the seed germination stage. Conclusions The results of this study may be helpful to further study the function of TaADH genes and determine the candidate gene for wheat stress resistance breeding.

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Pear (Pyrus spp.) Breeding

Silva

Villa

Grimaldi

et al. 2018

Advances in Plant Breeding Strategies: Fruits

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Identification and expression patterns of alcohol dehydrogenase genes involving in ester volatile biosynthesis in pear fruit

Cited by 19 publications

References 33 publications

Chromosome-scale genome assembly provides insights into the evolution and flavor synthesis of passion fruit (Passiflora edulis Sims)

Chromosome-scale genome assembly provides insights into the evolution and flavor synthesis of passion fruit (Passiflora edulis Sims)

Alcohol dehydrogenase (ADH) genes family in wheat (Triticum aestivum): Genome-wide identification, characterization, phylogenetic relationship and expression patterns

Pear (Pyrus spp.) Breeding

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