Identification and expression patterns of extracellular matrix-associated genes fibropellin-ia and tenascin involved in regeneration of sea cucumber Apostichopus japonicus

Ba, Huazhong; Yao, Feng; Yang, Lei; Qin, Tong; Luan, Hong; Li, Zhengmin; Zou, Xiangyang; Lin, Hui

doi:10.1016/j.gene.2015.03.071

Cited by 18 publications

(11 citation statements)

References 33 publications

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“…Recently, it was shown that the holothurian A . japonicus has tenascin-like proteins, which contain the EGF and FBG domains [ 90 ]. Nevertheless, according to Hynes [ 91 ], all these domains are ancient in origin and occur in many of animals; however, the combination typical of tenascins is observed only in chordates.…”

Section: Resultsmentioning

confidence: 99%

Molecular mechanisms of fission in echinoderms: Transcriptome analysis

2018

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Echinoderms are capable of asexual reproduction by fission. An individual divides into parts due to changes in the strength of connective tissue of the body wall. The structure of connective tissue and the mechanisms of variations in its strength in echinoderms remain poorly studied. An analysis of transcriptomes of individuals during the process of fission provides a new opportunity to understand the mechanisms of connective tissue mutability. In the holothurian Cladolabes schmeltzii, we have found a rather complex organization of connective tissue. Transcripts of genes encoding a wide range of structural proteins of extracellular matrix, as well as various proteases and their inhibitors, have been discovered. All these molecules may constitute a part of the mechanism of connective tissue mutability. According to our data, the extracellular matrix of echinoderms is substantially distinguished from that of vertebrates by the lack of elastin, fibronectins, and tenascins. In case of fission, a large number of genes of transcription factors and components of different signaling pathways are expressed. Products of these genes are probably involved in regulation of asexual reproduction, connective tissue mutability, and preparation of tissues for subsequent regeneration. It has been shown that holothurian tensilins are a special group of tissue inhibitors of metalloproteinases, which has formed within the class Holothuroidea and is absent from other echinoderms. Our data can serve a basis for the further study of the mechanisms of extracellular matrix mutability, as well as the mechanisms responsible for asexual reproduction in echinoderms.

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Section: Resultsmentioning

confidence: 99%

Molecular mechanisms of fission in echinoderms: Transcriptome analysis

2018

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“…These proteins play an important role in the structural integrity of connective tissues in vertebrates [78,79]. In some of holothurians, transcripts have been found that are blasted as tenascin-like proteins [71,72,80]. These contigs encode the domains characteristic of tenascins, EGF, FBG, and TILa.…”

Section: Structural Components Of Connective Tissuementioning

confidence: 99%

Molecular Aspects of Regeneration Mechanisms in Holothurians

Dolmatov

2021

Genes

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Holothurians, or sea cucumbers, belong to the phylum Echinodermata. They show good regenerative abilities. The present review provides an analysis of available data on the molecular aspects of regeneration mechanisms in holothurians. The genes and signaling pathways activated during the asexual reproduction and the formation of the anterior and posterior parts of the body, as well as the molecular mechanisms that provide regeneration of the nervous and digestive systems, are considered here. Damage causes a strong stress response, the signs of which are recorded even at late regeneration stages. In holothurian tissues, the concentrations of reactive oxygen species and antioxidant enzymes increase. Furthermore, the cellular and humoral components of the immune system are activated. Extracellular matrix remodeling and Wnt signaling play a major role in the regeneration in holothurians. All available morphological and molecular data show that the dedifferentiation of specialized cells in the remnant of the organ and the epithelial morphogenesis constitute the basis of regeneration in holothurians. However, depending on the type of damage, the mechanisms of regeneration may differ significantly in the spatial organization of regeneration process, the involvement of different cell types, and the depth of reprogramming of their genome (dedifferentiation or transdifferentiation).

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“…High affinity of vertebrate avidin to biotin is generally thought to inhibit bacterial growth, whereas the avidin-like domain of sea urchin fibropellin does not bind to biotin and was adopted for oligomerization (Itai et al, 2005). However, tissue-specific expression of avidin-like genes during early and late stages of intestine regeneration in the sea cucumber A. japonicus has been reported (Ba et al, 2015), suggesting an important role for starfish avidin-like protein in response to BL injury.…”

Section: Binding and Protein Binding Categorymentioning

confidence: 99%

Injury affects coelomic fluid proteome of the common starfishAsterias rubens

Shabelnikov

Bobkov

Sharlaimova

et al. 2019

Journal of Experimental Biology

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Echinoderms, possessing outstanding regenerative capabilities, provide a unique model system for the study of response to injury. However, little is known about the proteomic composition of coelomic fluid, an important biofluid circulating throughout the animal's body and reflecting the overall biological status of the organism. In this study, we used LC-MALDI tandem mass spectrometry to characterize the proteome of the cell-free coelomic fluid of the starfish Asterias rubens and to follow the changes occurring in response to puncture wound and blood loss. In total, 91 proteins were identified, of which 61 were extracellular soluble and 16 were bound to the plasma membrane. The most represented functional terms were 'pattern recognition receptor activity' and 'peptidase inhibitor activity'. A series of candidate proteins involved in early response to injury was revealed. Ependymin, β-microseminoprotein, serum amyloid A and avidin-like proteins, which are known to be involved in intestinal regeneration in the sea cucumber, were also identified as injuryresponsive proteins. Our results expand the list of proteins potentially involved in defense and regeneration in echinoderms and demonstrate dramatic effects of injury on the coelomic fluid proteome.

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Identification and expression patterns of extracellular matrix-associated genes fibropellin-ia and tenascin involved in regeneration of sea cucumber Apostichopus japonicus

Cited by 18 publications

References 33 publications

Molecular mechanisms of fission in echinoderms: Transcriptome analysis

Molecular mechanisms of fission in echinoderms: Transcriptome analysis

Molecular Aspects of Regeneration Mechanisms in Holothurians

Injury affects coelomic fluid proteome of the common starfishAsterias rubens

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