Identification and expression patterns of two TRPV channel genes in antennae and Johnston's organ of the dengue and Zika virus vector mosquito, Aedes aegypti

Na, Young‐Eun; Jung, Je Won; Kwon, Hyung Wook

doi:10.1016/j.aspen.2016.06.009

Cited by 5 publications

(1 citation statement)

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“…Our found significant correlation of blood volume ingested with the number of activity bouts (Table 1) still held for blood containing capsaicin (Figs. 5G and 6B), which acts on external transient receptor potential (TRP) channels 68 known to be expressed in the periphery 26,36,69,70 . The flyPad system showed that Aedes females were more sensitive at the periphery to capsaicin than caffeine (Fig.…”

Section: Discussionmentioning

confidence: 99%

Automated analysis of feeding behaviors of females of the mosquito Aedes aegypti using a modified flyPAD system

Henriques-Santos,

Xiong,

Pietrantonio

2023

Sci Rep

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Mosquitoes present a global health challenge due to their ability to transmit human and animal pathogens upon biting and blood feeding. The investigation of tastants detected by mosquitoes and their associated feeding behaviors is needed to answer physiological and ecological questions that could lead to novel control methods. A high-throughput system originally developed for research in fruit flies feeding behavior, the flyPAD, was adapted and tested for behaviors associated with the interaction or consumption of liquid diets offered to females of the mosquito Aedes aegypti Liverpool strain. Females were given water, sucrose solution and sheep blood in choice and non-choice assays. The volume ingested was evaluated with fluorescein. The placement of the system on a heated surface allowed blood consumption, and without females puncturing a membrane. The flyPAD system recorded nine feeding behavioral variables, of which the number of sips and number of activity bouts correlated with meal volume ingested for both sucrose solution and blood. The adaptation to mosquitoes of the flyPAD system differentiated feeding behavior variables between two feeding deterrents, capsaicin, and caffeine. The flyPAD has potential to quickly assess diverse tastants in both sucrose and blood and may contribute to characterizing more precisely their mode of action.

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