Identification and Expression Profile Analysis of the OSCA Gene Family Related to Abiotic and Biotic Stress Response in Cucumber

Abstract

Calcium ions are important second messengers, playing an important role in the signal transduction pathways. Hyperosmolality gated calcium-permeable channels (OSCA) gene family members play critical modulating roles in response to osmotic-related abiotic stress as well as other abiotic and biotic stresses, which has been reported in many plant species such as Arabidopsis, rice, maize, and wheat. However, there has been no report about the identification and expression profile of the OSCA genes in cucumber. In this study, a total of nine OSCA genes were identified, which are unevenly distributed on the six chromosomes of cucumber. Phylogenetic analysis revealed that the OSCAs of cucumber, Arabidopsis, rice and maize were clustered into four clades. The motif arrangement of CsOSCAs was strongly conserved, and the CsOSCA genes in each group had similar genetic structure. A total of 11 and 10 types of cis-elements related to hormone and stress, respectively, were identified in the promoter regions of CsOSCA genes. Gene expression analysis indicated that the CsOSCA genes have different expression patterns in various tissues, and some of them were regulated by three osmotic-related abiotic stresses (salt, drought and ABA) and three biotic stresses (powdery mildew, downy mildew, and root-knot nematode). As the first genome-wide identification and characterization of the OSCA gene family in cucumber, this study lays a foundation for research on the biological function and evolutionary process of this gene family, which is of great significance for exploiting stress resistant cucumber varieties.

Genome-wide investigation and expression analysis of OSCA gene family in response to abiotic stress in alfalfa

“…We systematically analyzed the phylogenetic relationships of OSCA proteins in alfalfa, Arabidopsis, C. arietinum, V. radiata and C. cajan. The OSCA proteins of alfalfa, Arabidopsis, C. arietinum, V. radiata and C. cajan were clustered into four phylogenetic clades (Figure 1), which was consistent with Solanum habrochaites (Miao et al, 2022), cucumber (Yang et al, 2022), Gossypium hirsutum (Yang et al, 2019), rice (Li et al, 2015) and maize (Cao et al, 2020), possibly due to the conservative evolution of OSCA genes. In addition, on each clade had AtOSCAs, MsOSCAs, CcOSCAs, CaOSCAs and VrOSCAs, suggesting that they might have a common ancestor.…”

“…In this study, the MsOSCA genes were distributed on each chromosome of alfalfa, but their distribution was not uniform, which is similar to that in most species (Jie et al, 2022;Yang et al, 2022). Gene duplication is one of the most important driving forces for the evolution of the genome and the genetics of a species (Magadum et al, 2013).…”

“…As the only osmoreceptors in plants, OSCAs are capable of responding to abiotic stress, such as salt and drought, and have been confirmed in a variety of crops, such as rice (Li et al, 2015), sunflower (Shan et al, 2023), cucumber (Yang et al, 2022), soybean (Liu et al, 2022) and maize (Cao et al, 2020). In Arabidopsis, OSCA1 functions as an osmotic sensing receptor that mediates the elevation of Ca 2+ concentration in response to osmotic stress (Liu et al, 2018b).…”

Genome-wide identification of the CYP82 gene family in cucumber and functional characterization of CsCYP82D102 in regulating resistance to powdery mildew

“…Cucumber transcriptome data ( ) was acquired from the Cucurbit Expression Atlas within the Cucurbit Genomics Database (CuGenDB). A detailed analysis of the expression profiles of 12 CYP82 genes was performed using the FeatureCounts R package, and these patterns were subsequently visualized as heatmaps utilizing TBtools ( Yang et al, 2022 ). Concurrently, heatmaps representing the expression patterns of CYP82 genes in response to PM treatment were generated using cucumber transcriptome data ( ) ( Yang et al, 2022 ).…”

Surviving a Double-Edged Sword: Response of Horticultural Crops to Multiple Abiotic Stressors

“…Based on this, OSCA1 can be regarded as a hyperosmotic sensor. It is worth mentioning that OSCA family members are also widely distributed in horticultural crops, such as cucumber (9 CsOSCA) and Solanum habrochaites (11 ShOSCA) [146][147][148]. We speculated that OSCA1 could also be a sensor in horticultural crops responding to hyperosmotic stress such as high temperature and high salt.…”