Identification and expression profiling of CLCuV-responsive transcripts in upland cotton (Gossypium hirsutum L.)

Abstract

CLCuV is one of the most serious causes of cotton damage for which no adequate remedy is available. The possible solution to this problem may be the transfer of resistant genes into the background of agronomical CLCuV-tolerant varieties. In response to viral infection, several plants may show qualitative and quantitative variations in gene expression. To study gene expression between diseased and tolerant plants, DDRT-PCR was performed. Upregulated gene expression was observed among cDNA transcripts screened through random primers. Seven transcripts have significant homology with known proteins (ribosomal protein, photosystem protein unit, membrane protein, and a predicted hypothetical protein). Quantitative real-time PCR showed varied levels of expression in tolerant as compared to CLCuV-diseased leaf samples. The potential candidate transcript DET1 was generated full length as GhLCVR. The deduced amino acid sequence, with a molecular mass of 16.1 kDa, contains a single open reading frame of 142 amino acids. The predicted amino acid sequence shares 33%-80% identities with Glycine max, Populus trichocarpa, Vitis vinifera, Zea mays, Ricinus communis, Arabidopsis thaliana, Picea sitchensis, Physcomitrella patens, and Staphylococcus aureus in descending order. This gene and other identified DETs could provide a valuable resource for the future improvement of cotton varieties against CLCuV infection.

Gene expression in response to Cotton Leaf Curl Virus infection in Gossypium hirsutum under variable environmental conditions

“…It is important to identify the certain genes families in G. hirsutum that show their involvement in defense related mechanisms to CLCuV (AHMAD et al 2011;AZAM et al 2013;FAROOQ et al 2014;SHAHID et al 2016). These genes either expressed or suppressed during pathogen infection and lead to activation of glucosinolates, reactive oxygen species, hypersensitive response, pathogenesis related genes and many other secondary metabolites pathways (AFTAB et al 2014;ALAM et al 2015).…”

Molecular and Biochemical Characterization of Cotton Epicuticular Wax in Defense Against Cotton Leaf Curl Disease

“…The aims of the present study was (1) to quantify the cuticular waxes and determine the biochemical composition of wax mutant GaWM3 in comparison with G. arboreum, G. hirsutum and G. harknessii, and (2) to determine the CLCuV titer and its correlation with quantity and composition of waxes through feeding of whiteflies on plants.…”