Identification and Extraction Optimization of Active Constituents in Citrus junos Seib ex TANAKA Peel and Its Biological Evaluation

Shim, Jung‐Hyun; Chae, Jung‐Il; Cho, Seung‐Sik

doi:10.3390/molecules24040680

Cited by 14 publications

(15 citation statements)

References 29 publications

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“…Our results for the antioxidant activity of rosmarinic acid via DPPH is consistent with prior work on Melissa officinalis [75]. Citrus junos extracts rich in naringin showed moderate antioxidant activities [76], but a different study documented weaker activities [77]. Cynaroside obtained from Elsholtzia bodinieri had strong antioxidant activities (via the DPPH assay) [78], and cryptochlorogenic and chlorogenic acids from Hibiscus sabdariffa showed strong antioxidant activities [79].…”

Section: Discussionsupporting

confidence: 90%

Polyphenol Profile and Antimicrobial and Cytotoxic Activities of Natural Mentha × piperita and Mentha longifolia Populations in Northern Saudi Arabia

et al. 2020

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New sources of polyphenols with anticancer, antioxidant, and antimicrobial properties in arid environments are critical for the development of alternative medicines and natural remedies. This study explored the polyphenol profiles and biological activities of methanolic leaf extracts from natural Mentha × piperita and Mentha longifolia populations in northern Saudi Arabia. Chromatographic analyses identified several polyphenols in M. × piperita including phenolic acids: rosmarinic acid (1547.6 mg/100 g DW (dry weight)), cryptochlorogenic acid (91.7 mg/100 g DW), and chlorogenic acid (69.4 mg/100 g DW), as well as flavonoids: naringin (328.8 mg/100 g DW) and cynaroside (162.8 mg/100 g DW). The major polyphenols in M. longifolia were: rosmarinic acid (781.6 mg/100 g DW), cryptochlorogenic acid (191.1 mg/100 g DW), p-coumaric acid (113.0 mg/100 g DW), m-coumaric acid (112.2 mg/100 g DW), and chlorogenic acid (63.8 mg/100 g DW). M. × piperita and M. longifolia leaf extracts had high antioxidant activities due to the major polyphenols (cynaroside, rosmarinic and cryptochlorogenic acids). M. × piperita had higher activities against different cancer cells than M. longifolia. Naringin, cryptochlorogenic acid, and rosmarinic acid had the highest activities against cancer cells. The leaf extracts had antibacterial effects against most bacteria species (Pseudomonas aeruginosa was most sensitive), which was attributed to the polyphenols. Antifungal activities were similarly broad (Aspergillus flavus was most sensitive) and attributed to naringin, cryptochlorogenic acid, and caffeic acid. Populations of M. × piperita and M. longifolia in Northern Riyadh may be a valuable source of natural biologically active compounds.

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Section: Discussionsupporting

confidence: 90%

Polyphenol Profile and Antimicrobial and Cytotoxic Activities of Natural Mentha × piperita and Mentha longifolia Populations in Northern Saudi Arabia

et al. 2020

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“…As far as biological activity is concerned, yuzu is certainly the most investigated among the selected Citrus species. The fruit has been examined for its antiaging (Adhikari et al., 2017; Shim et al., 2019), neuroprotective (Yang et al., 2013), lipid‐ and cholesterol‐lowering (Kim et al., 2013; Sharma et al., 2019; Shin et al., 2016), anti‐inflammatory (Abe et al., 2018), and osteogenic activities (Jeon et al., 2016) both as a plant complex and as a source of promising isolated bioactive compounds. Moreover, it is the only fruit that has been clinically investigated, whereas the other ones have been studied mostly by in vitro cell‐free and cell‐based assays.…”

Section: Discussionmentioning

confidence: 99%

“…The 80% ethanol extract showed the highest total phenols content, 25.44 mg of gallic acid equivalents (GAE)/g of DW), as well as the greatest antioxidant activity, with an IC 50 of 1,042.37 µg/mL in DPPH assay and a good reducing power on ferrous ions expressed as 24.99 µg of vitamin C equivalents/100 µg of dry extract. The 100% ethanol extract (1 mg/mL) had the greatest xanthine oxidase inhibitory activity (55.74%), whereas aqueous and 20% ethanol extracts showed the highest inhibitory activity on elastase (56.3% and 61.4%, respectively) (Shim et al., 2019).…”

Section: Biological Activitymentioning

confidence: 99%

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New insights into Citrus genus: From ancient fruits to new hybrids

et al. 2020

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Citrus fruits are among the most ancient known and have always attracted the interest of humans, from both a nutritional and a health point of view. Even though there is substantial scientific literature regarding the Citrus genus and associated isolated bioactive compounds, there are still few studies on the botanical features, the phytochemical profiles, and the biological activities for some ancient Citrus fruits as well as for new hybrids that have become part of the international market. We have witnessed both the rediscovery and revaluation of some cultivated ancient fruits in some limited areas and the introduction of new hybrids destined to the food, ornamental, and food design market. This is the first review that sheds light on the botanical and chemical features as well as on the biological properties of Citrus fruits that are under-investigated, localizing them in an international context. The manuscript focuses in particular on five under-investigated species: Citrus australasica F. Muell. (Finger lime), Citrus medica L. var. sarcodactylis Swingle (Buddha's hand), Citrus junos Sieb ex Tan. (Yuzu), Citrus limon (L.) Osbeck var. pompia Camarda (Pompia), and Citrus lumia Risso (Lumia).

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“…Here, we developed and fully validated a simple and efficient HPLC method for quantifying SBN in rat plasma. The linearity, sensitivity, precision, accuracy, recovery, matrix effect, and stability of this HPLC method were assessed [14,15]. Next, the developed bioanalytical method was applied to investigate the comparative pharmacokinetics of SBN following the oral administration of commercially available functional food products of silymarin that were manufactured by four different pharmaceutical companies located in South Korea.…”

Section: Introductionmentioning

confidence: 99%

A Simple HPLC Method for the Quantitative Determination of Silybin in Rat Plasma: Application to a Comparative Pharmacokinetic Study on Commercial Silymarin Products

Han

Seo

et al. 2019

Molecules

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Silybin (SBN) is a major active constituent of silymarin, a mixture of flavonoids found in fruits and seeds of milk thistle. The aim of this study was to describe a simple bioanalytical method for quantifying SBN in rat plasma. A simple protein deproteinization procedure with acetonitrile (ACN) was employed for plasma sample preparation. A reversed column and gradient elution of a mobile phase (mixture of phosphate buffer (pH 5.0) and ACN) were used for chromatographic separation. The selectivity, linearity (50–5000 ng/mL), precision, accuracy, recovery, matrix effect, and stability for this method were validated as per the current Food and Drug Administration (FDA) guidelines. Our method for SBN was applied to a comparative pharmacokinetic study on four different commercial silymarin products. This in vivo rat study demonstrated that product #4 significantly enhanced the relative oral bioavailability of SBN, as compared to product #1–3. Therefore, the bioanalytical method proposed herein could serve as a promising alternative for preclinical pharmacokinetic studies on silymarin products and, by extension, clinical use after partial modification and validation.

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Identification and Extraction Optimization of Active Constituents in Citrus junos Seib ex TANAKA Peel and Its Biological Evaluation

Cited by 14 publications

References 29 publications

Polyphenol Profile and Antimicrobial and Cytotoxic Activities of Natural Mentha × piperita and Mentha longifolia Populations in Northern Saudi Arabia

Polyphenol Profile and Antimicrobial and Cytotoxic Activities of Natural Mentha × piperita and Mentha longifolia Populations in Northern Saudi Arabia

New insights into Citrus genus: From ancient fruits to new hybrids

A Simple HPLC Method for the Quantitative Determination of Silybin in Rat Plasma: Application to a Comparative Pharmacokinetic Study on Commercial Silymarin Products

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