In this study, the bacterial community of galchi-baechu kimchi was determined using culture-based and culture-independent techniques (next generation sequencing:NGS), and showed discrepancies between results.
Weissella koreensis
and
Pediococcus inopinatus
were the dominant species according to the NGS results, while
Bacillus
species and
P. inopinatus
were dominant in the culture-dependent analysis. To identify safe starter candidates, sixty-five
Bacillus
strains isolated from galchi-baechu kimchi using culture-dependent methods were evaluated for their antibiotic resistance, presence of toxin genes, and hemolytic activity. Strains were then assessed for salt tolerance and protease and lipase activity. As a result, four strains-
B. safensis
GN5_10,
B. subtilis
GN5_19,
B. velezensis
GN5_25, and
B. velezensis
GT8-were selected as safe starter candidates for use in fermented foods.