Identification and Fine Mapping of Candidate Gene for Yellow Leaf Mutant (ygl54) Exhibiting Yellow Leaf Colour in Rice

Khan, Asadullah; Jalil, Sanaullah; Cao, Huấn; Sunusi, Mustapha; Tsago, Yohannes; Chen, J.; Shi, Chao; Jin, Xiaogang

doi:10.1134/s1021443721060078

Cited by 4 publications

(1 citation statement)

References 27 publications

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“…The Pn, Gs, and Tr of ygl14 were significantly lower than those of the WT, however, the intercellular CO 2 concentration was significantly increased 13 . The photosynthetic parameters in the ygl54 mutant were all significantly decreased compared to those in the WT 25 . Chlorophyll fluorescence parameters can effectively reflect the absorption, utilization, dissipation and distribution of light energy by plants.…”

Section: Discussionmentioning

confidence: 83%

Physiological and transcriptomic analysis of a yellow leaf mutant in watermelon

Zhang

et al. 2023

Sci Rep

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Leaf color mutants are important materials for studying chloroplast and photomorphogenesis, and can function as basic germplasms for genetic breeding. In an ethylmethanesulfonate mutagenesis population of watermelon cultivar “703”, a chlorophyll-deficient mutant with yellow leaf (Yl2) color was identified. The contents of chlorophyll a, chlorophyll b, and carotenoids in Yl2 leaves were lower than those in wild-type (WT) leaves. The chloroplast ultrastructure in the leaves revealed that the chloroplasts in Yl2 were degraded. The numbers of chloroplasts and thylakoids in the Yl2 mutant were lower, resulting in lower photosynthetic parameters. Transcriptomic analysis identified 1292 differentially expressed genes, including1002 upregulated and 290 downregulated genes. The genes involved in chlorophyll biosynthesis (HEMA, HEMD, CHL1, CHLM, and CAO) were significantly downregulated in the Yl2 mutant, which may explain why chlorophyll pigment content was lower than that in the WT. Chlorophyll metabolism genes such as PDS, ZDS and VDE, were upregulated, which form the xanthophyll cycle and may protect the yellow‒leaves plants from photodamage. Taken together, our findings provide insight into the molecular mechanisms of leading to leaf color formation and chloroplast development in watermelon.

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Section: Discussionmentioning

confidence: 83%

Physiological and transcriptomic analysis of a yellow leaf mutant in watermelon

Zhang

et al. 2023

Sci Rep

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Ethyl methanesulfonate (EMS) mediated dwarfing mutation provides a basis for CaCO₃ accumulation by enhancing photosynthetic performance in Ceratostigma willmottianum Stapf

Guo,

Lai,

Lei

et al. 2024

Plant Biol J

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Ceratostigma willmottianum Stapf is a unique chalk gland (salt‐excreting) plant from China, with a salt gland structure that excretes white crystals of calcium carbonate (CaCO3), which has potential biomineralization and carbon sequestration functions. Due to the narrow distribution of wild germplasm resources, there is a lack of diversity of new varieties to satisfy commercial development and scientific exploration. Therefore, we used ethyl methanesulfonate (EMS) mutagenesis to obtain new dwarf mutant germplasm, and analysed it in terms of morphology, growth, photosynthesis, salt glands, and excretion traits. All four dwarfing mutant strains (DM1, DM2, DM3, and DM4) exhibited extreme dwarfing (62.28%, 62.28%, 74.55% and 61.68% reduction in plant height, respectively), faster growth, increased belowground root biomass, and earlier bud differentiation and flowering. Photosynthetic capacity was enhanced: chlorophyll content, maximum quantum yield of PSII (Fv/Fm), effective quantum yield of PSII (ΦPSII), photochemical quenching coefficient (qP), electron transfer rate (ETR), net photosynthesis (Pn), intercellular CO2 concentration (Ci), stomatal conductance (Gs), and transpiration (Tr), were significantly higher in leaves of DM mutants. The density of salt glands per unit leaf area and average Ca2+ excretion rate of individual salt glands increased significantly (especially in DM2), and CaCO3 accumulation per unit leaf area was 28.57% higher than that of the wild type. Pearson correlation analysis showed that photosynthetic capacity was significantly and positively correlated with CaCO3 excretion. The above study not only provided enriched new germplasm of C. willmottianum, but also important research material for studying the mechanism of CaCO3 excretion by salt glands and carbon sequestration capacity of biomineralization.

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Comparative transcriptome analysis identified ChlH and POLGAMMA2 in regulating yellow-leaf coloration in Forsythia

Zhang

Jia

et al. 2022

Front. Plant Sci.

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Leaf color is one of the most important features for plants used for landscape and ornamental purposes. However, the regulatory mechanism of yellow leaf coloration still remains elusive in many plant species. To understand the complex genetic mechanism of yellow-leaf Forsythia, we first compared the pigment content and leaf anatomical structure of yellow-leaf and green-leaf accessions derived from a hybrid population. The physiological and cytological analyses demonstrated that yellow-leaf progenies were chlorophyll deficient with defected chloroplast structure. With comparative transcriptome analysis, we identified a number of candidate genes differentially expressed between yellow-leaf and green-leaf Forsythia plants. Among these genes, we further screened out two candidates, ChlH (magnesium chelatase Subunit H) and POLGAMMA2 (POLYMERASE GAMMA 2), with consistent relative-expression pattern between different colored plants. To verify the gene function, we performed virus-induced gene silencing assays and observed yellow-leaf phenotype with total chlorophyll content reduced by approximately 66 and 83% in ChlH-silenced and POLGAMMA2-silenced plants, respectively. We also observed defected chloroplast structure in both ChlH-silenced and POLGAMMA2-silenced Forsythia. Transient over-expression of ChlH and POLGAMMA2 led to increased chlorophyll content and restored thylakoid architecture in yellow-leaf Forsythia. With transcriptome sequencing, we detected a number of genes related to chlorophyll biosynthesis and chloroplast development that were responsive to the silencing of ChlH and POLGAMMA2. To summarize, ChlH and POLGAMMA2 are two key genes that possibly related to yellow-leaf coloration in Forsythia through modulating chlorophyll synthesis and chloroplast ultrastructure. Our study provided insights into the molecular aspects of yellow-leaf Forsythia and expanded the knowledge of foliage color regulation in woody ornamental plants.

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Identification and Fine Mapping of Candidate Gene for Yellow Leaf Mutant (ygl54) Exhibiting Yellow Leaf Colour in Rice

Cited by 4 publications

References 27 publications

Physiological and transcriptomic analysis of a yellow leaf mutant in watermelon

Physiological and transcriptomic analysis of a yellow leaf mutant in watermelon

Ethyl methanesulfonate (EMS) mediated dwarfing mutation provides a basis for CaCO₃ accumulation by enhancing photosynthetic performance in Ceratostigma willmottianum Stapf

Comparative transcriptome analysis identified ChlH and POLGAMMA2 in regulating yellow-leaf coloration in Forsythia

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Identification and Fine Mapping of Candidate Gene for Yellow Leaf Mutant (ygl54) Exhibiting Yellow Leaf Colour in Rice

Cited by 4 publications

References 27 publications

Physiological and transcriptomic analysis of a yellow leaf mutant in watermelon

Physiological and transcriptomic analysis of a yellow leaf mutant in watermelon

Ethyl methanesulfonate (EMS) mediated dwarfing mutation provides a basis for CaCO3 accumulation by enhancing photosynthetic performance in Ceratostigma willmottianum Stapf

Comparative transcriptome analysis identified ChlH and POLGAMMA2 in regulating yellow-leaf coloration in Forsythia

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Ethyl methanesulfonate (EMS) mediated dwarfing mutation provides a basis for CaCO₃ accumulation by enhancing photosynthetic performance in Ceratostigma willmottianum Stapf