Identification and fine mapping of alien fragments associated with enhanced grain weight from Agropyron cristatum chromosome 7P in common wheat backgrounds

Sun, Yangyang; Lv, Mingjie; Zhou, Shumin; Lu, Yuqing; Liu, Weihua; Yang, Xinming; Li, Xiuquan; Zhang, Jinpeng; Xu, Lai; Li, Lihui

doi:10.1007/s00122-021-03927-7

Cited by 22 publications

(18 citation statements)

References 54 publications

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“…Species of Agropyron also possess a lot of valuable traits, such as tolerance to drought and cold, resistance to diseases, and high yield (Asay et al, 1985;Dong et al, 1992;Lu et al, 2015). They are high-quality forage for grassland improvement and valuable genetic resources for wheat improvement (Ochoa et al, 2015;Sun et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Analysis of Chloroplast Genomes Provides Insights Into the Evolution of Agropyron

et al. 2022

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Plants of the Agropyron genus are important pasture resources, and they also play important roles in the ecological restoration. Chloroplast genomes are inherited from maternal parents, and they are important for studying species taxonomy and evolution. In this study, we sequenced the complete chloroplast genomes of five typical species of the Agropyron genus (eg., A. cristatum × A. desertorum Fisch. Schult, A. desertorum, A. desertorum Fisch. Schult. cv. Nordan, A. michnoi Roshev, and A. mongolicum Keng) using the Illumina NovaSeq platform. We found that these five chloroplast genomes exhibit a typical quadripartite structure with a conserved genome arrangement and structure. Their chloroplast genomes contain the large single-copy regions (LSC, 79,613 bp-79,634 bp), the small single-copy regions (SSC, 12,760 bp-12,768 bp), and the inverted repeat regions (IR, 43,060 bp-43,090 bp). Each of the five chloroplast genomes contains 129 genes, including 38 tRNA genes, eight rRNA genes, and 83 protein-coding genes. Among them, the genes trnG-GCC, matK, petL, ccsA, and rpl32 showed significant nucleotide diversity in these five species, and they may be used as molecular markers in taxonomic studies. Phylogenetic analysis showed that A. mongolicum is closely related to A. michnoi, while others have a closer genetic relationship with the Triticum genus.

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Section: Introductionmentioning

confidence: 99%

Analysis of Chloroplast Genomes Provides Insights Into the Evolution of Agropyron

et al. 2022

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“…In this case, A. cristatum has received extensive amounts of attention from breeders because of the agronomic traits of multiple owers and spikelets. In recent years, 6P and 7P chromosome increase GNS and TGW were discovered, respectively (Wu et al 2006;Sun et al 2021), and many introgression lines, such as PB3504 and PB3228, that carry high-yield genes have been used for breeding high-yielding wheat (Chen et al 2016;Liu et al 2020). Furthermore, the use of several high-yield varieties, such as PB151 and Chuanmai93 has been promoted in recent years.…”

Section: Discussionmentioning

confidence: 99%

Introgression of Agropyron cristatum genes to counteract the negative relationship between grain number and grain weight of wheat

Lin

Zhou

Liang

et al. 2022

Preprint

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Agropyron cristatum (2n = 4X = 28, PPPP), which harbour many high-yield and disease-resistance genes, is a promising donor for wheat improvement. Narrow genetic diversity and the trade-off between grain weight and grain number have become bottlenecks for increasing grain yield. In this study, WAT650l, a new translocation line was generated via radiation (60Co-γ rays)-based mutation induction of chromosome 6P addition line 4844-12. Cytological analysis and molecular marker analysis revealed that WAT650l was a 6PL (bin ~ 12–17)·5BS-5BL translocation line. Assessment of agronomic traits and analysis of the BC4F2 and BC5F2 populations suggested that the 6PL terminal chromosome segment in WAT650l resulted in increased flag leaf length, plant height (PH), spikelet number per spike (SNS), kernel number per spikelet (KNS), grain number per spike (GNS) (average increased by 14.07 grains) and thousand-grain weight (TGW) (average increased by 4.31 g) during the growing seasons of 2020–2021 and 2021–2022. Additionally, the increased GNS locus and high-TGW locus of WAT650l were mapped to 6PL (16–17) and 6PL (12–13), respectively, by genetic population analysis of three translocation lines. In summary, by using high-yield genes of A. cristatum and overcoming the negative relationship between GNS and TGW associated with wheat breeding, we provide a valuable germplasm resource for broadening the genetic base of wheat.

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“…Leaf tissues were collected from inoculated and uninoculated plants at five time points (6,12,24,48, and 72 hpi) for three biological replicates. The leaf samples were instantly frozen and ground into a fine powder in liquid nitrogen in a mortar and pestle to isolate RNA using an RNA Isolation Kit (Zoman Biotechnology, Beijing, China).…”

Section: Rna Sequencing and Transcriptome Analysismentioning

confidence: 99%

“…tauschii [10,21] have been successfully applied in wheat breeding. Agropyron cristatum (A. cristatum) (2n = 4x = 28, PPPP), a wild relative of wheat, has resistance to some major diseases, such as wheat rust, powdery mildew, and yellow dwarfing; thus, this species can be used as an excellent genetic source for wheat [22][23][24][25][26].…”

Section: Introductionmentioning

confidence: 99%

Comparative Transcriptome Analysis Reveals the Gene Expression and Regulatory Characteristics of Broad-Spectrum Immunity to Leaf Rust in a Wheat–Agropyron cristatum 2P Addition Line

Liu

et al. 2022

IJMS

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Wheat leaf rust (caused by Puccinia triticina Erikss.) is among the major diseases of common wheat. The lack of resistance genes to leaf rust has limited the development of wheat cultivars. Wheat–Agropyron cristatum (A. cristatum) 2P addition line II-9-3 has been shown to provide broad-spectrum immunity to leaf rust. To identify the specific A. cristatum resistance genes and related regulatory pathways in II-9-3, we conducted a comparative transcriptome analysis of inoculated and uninoculated leaves of the resistant addition line II-9-3 and the susceptible cultivar Fukuhokomugi (Fukuho). The results showed that there were 66 A. cristatum differentially expressed genes (DEGs) and 1389 wheat DEGs in II-9-3 during P. triticina infection. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment and gene set enrichment analysis (GSEA) revealed that the DEGs of II-9-3 were associated with plant–pathogen interaction, MAPK signaling pathway–plant, plant hormone signal transduction, glutathione metabolism, and phenylpropanoid biosynthesis. Furthermore, many defense-related A. cristatum genes, such as two NLR genes, seven receptor kinase-encoding genes, and four transcription factor-encoding genes, were identified. Our results indicated that the key step of resistance to leaf rust involves, firstly, the gene expression of chromosome 2P upstream of the immune pathway and, secondly, the effect of chromosome 2P on the co-expression of wheat genes in II-9-3. The disease resistance regulatory pathways and related genes in the addition line II-9-3 thus could play a critical role in the effective utilization of innovative resources for leaf rust resistance in wheat breeding.

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Identification and fine mapping of alien fragments associated with enhanced grain weight from Agropyron cristatum chromosome 7P in common wheat backgrounds

Cited by 22 publications

References 54 publications

Analysis of Chloroplast Genomes Provides Insights Into the Evolution of Agropyron

Analysis of Chloroplast Genomes Provides Insights Into the Evolution of Agropyron

Introgression of Agropyron cristatum genes to counteract the negative relationship between grain number and grain weight of wheat

Comparative Transcriptome Analysis Reveals the Gene Expression and Regulatory Characteristics of Broad-Spectrum Immunity to Leaf Rust in a Wheat–Agropyron cristatum 2P Addition Line

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