Identification and functional analysis of the ICK gene family in maize

Xiao, Qianlin; Zhang, Chunxia; Li, Hui; Wei, Bin; Wang, Yongbin; Huang, Huanhuan; Li, Yangping; Yu, Guowu; Liu, Hanmei; Zhang, Junjie; Liu, Yanping; Hu, Yu‐Feng; Huang, Yubi

doi:10.1038/srep43818

Cited by 6 publications

(5 citation statements)

References 48 publications

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“…Interestingly, PtrCYCD1;4, PtrCYCD5;3, and PtrCYCD6 in poplar also have no LxCxE motifs [20]. In addition, CYCDs also contain a conserved cyclin box, which is the binding domain of CDK or inhibitors of CDK (ICK) and forms a CDK/ICK-Cyclin complex [56,57]. It has also been reported that some CYCD cyclins can be rapidly degraded, which depends mainly on the presence of PEST sequences [2,58].…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Analysis of the D-type Cyclin Gene Family Reveals Differential Expression Patterns and Stem Development in the Woody Plant Prunus mume

Zheng

Zhuo

et al. 2019

Forests

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Cyclins, a prominent class of cell division regulators, play an extremely important role in plant growth and development. D-type cyclins (CYCDs) are the rate-limiting components of the G1 phase. In plants, studies of CYCDs are mainly concerned with herbaceous plants, yet little information is available about these genes in perennial woody plants, especially ornamental plants. Here, twelve Prunus mume CYCD (PmCYCDs) genes are identified and characterized. The PmCYCDs were named on the basis of orthologues in Arabidopsis thaliana and Oryza sativa. Gene structure and conserved domains of each subgroup CYCDs was similar to that of their orthologues in A. thaliana and O. sativa. However, PmCYCDs exhibited different tissue-specific expression patterns in root, stem, leaf, bud, and fruit organs. The results of qRT-PCR showed that all PmCYCDs, except PmCYCD5;2 and PmCYCD7;1, were primarily highly expressed in leaf buds, shoots, and stems. In addition, the transcript levels of PmCYCD genes were analyzed in roots under different treatments, including exogenous applications of NAA, 6-BA, GA3, ABA, and sucrose. Interestingly, although PmCYCDs were induced by sucrose, the extent of gene induction among PmCYCD subgroups varied. The induction of PmCYCD1;2 by hormones depended on the presence of sucrose. PmCYCD3;1 was stimulated by NAA, and induction was strengthened when sugar and hormones were applied together. Taken together, our study demonstrates that PmCYCDs are functional in plant stem development and provides a basis for selecting members of the cyclin gene family as candidate genes for ornamental plant breeding.

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Section: Discussionmentioning

confidence: 99%

Genome-Wide Analysis of the D-type Cyclin Gene Family Reveals Differential Expression Patterns and Stem Development in the Woody Plant Prunus mume

Zheng

Zhuo

et al. 2019

Forests

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“…As a crucial negative regulator of the cell cycle, the KRP genes have been identified, and a considerable amount of research have been studied in several plant species and have been demonstrated to play significant roles on plant development and in response to various stressors (Polyn et al, 2015;Kumar and Larkin, 2017;Vieira and de Almeida Engler, 2017;Xiao et al, 2017;Sahu et al, 2021). In this study, a comprehensive analysis of genes encoding KRP proteins in the soybean genome was performed, resulting in the identification of nine KRP family members.…”

Section: Characterization Of the Soybean Krp Gene Familymentioning

confidence: 99%

“…Plant CKIs have two distinct families: KIP-RELATED PROTEINS (KRPs) and SIAMESE-RELATED proteins (SMRs). Plant KRP and SMR members have been identified and analyzed in several species, including Arabidopsis, tobacco, rice, maize, and tomato (De Veylder et al, 2001;Jasinski et al, 2002;Bisbis et al, 2006;Guo et al, 2007;Xiao et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Characterization of the soybean KRP gene family reveals a key role for GmKRP2a in root development

Guo

Chen²,

Yang³

et al. 2023

Front. Plant Sci.

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Kip-related proteins (KRPs), as inhibitory proteins of cyclin-dependent kinases, are involved in the growth and development of plants by regulating the activity of the CYC-CDK complex to control cell cycle progression. The KRP gene family has been identified in several plants, and several KRP proteins from Arabidopsis thaliana have been functionally characterized. However, there is little research on KRP genes in soybean, which is an economically important crop. In this study, we identified nine GmKRP genes in the Glycine max genome using HMM modeling and BLASTP searches. Protein subcellular localization and conserved motif analysis showed soybean KRP proteins located in the nucleus, and the C-terminal protein sequence was highly conserved. By investigating the expression patterns in various tissues, we found that all GmKRPs exhibited transcript abundance, while several showed tissue-specific expression patterns. By analyzing the promoter region, we found that light, low temperature, an anaerobic environment, and hormones-related cis-elements were abundant. In addition, we performed a co-expression analysis of the GmKRP gene family, followed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) set enrichment analysis. The co-expressing genes were mainly involved in RNA synthesis and modification and energy metabolism. Furthermore, the GmKRP2a gene, a member of the soybean KRP family, was cloned for further functional analysis. GmKRP2a is located in the nucleus and participates in root development by regulating cell cycle progression. RNA-seq results indicated that GmKRP2a is involved in cell cycle regulation through ribosome regulation, cell expansion, hormone response, stress response, and plant pathogen response pathways. To our knowledge, this is the first study to identify and characterize the KRP gene family in soybean.

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“…The expression vector, pCAMBIA2300-35S-eGFP (Xiao et al, 2017), containing an enhanced green fluorescent protein (eGFP) under the control of CaMV 35S promoter, was used for the analysis of subcellular localization of ZmTCP7 in maize endosperm protoplast system (MEPS; Hu et al, 2020). The ZmTCP7 coding sequence without stop codon was amplified and inserted into the expression vector at the KpnI and XbaI sites to create the fusion construct pCAMBIA2300-35S-ZmTCP7-eGFP.…”

Section: Subcellular Location Of Zmtcp7mentioning

confidence: 99%

The novel ZmTCP7 transcription factor targets AGPase-encoding gene ZmBt2 to regulate storage starch accumulation in maize

Ajayo

Wang

et al. 2022

Front. Plant Sci.

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The process of starch biosynthesis is a major developmental event that affects the final grain yield and quality in maize (Zea mays L.), and transcriptional regulation plays a key role in modulating the expression of the main players in the pathway. ZmBt2, which encodes the small subunits of AGPase, is a rate-controlling gene of the pathway; however, much remains unknown about its transcriptional regulation. Our earlier study identifies a short functional fragment of ZmBt2 promoter (394-bp), and further shows it contains multiple putative cis-acting regulatory elements, demonstrating that several transcription factors may govern ZmBt2 expression. Here, we identified a novel TCP transcription factor (TF), ZmTCP7, that interacted with the functional fragment of the ZmBt2 promoter in a yeast one hybrid screening system. We further showed that ZmTCP7 is a non-autonomous TF targeted to the nucleus and predominantly expressed in maize endosperm. Using promoter deletion analyzes by transient expression in maize endosperm protoplasts combined with electrophoretic mobility shift assays, we found that ZmTCP7 bound to GAACCCCAC elements on the ZmBt2 promoter to suppress its expression. Transgenic overexpression of ZmTCP7 in maize caused a significant repression of ZmBt2 transcription by ~77.58%, resulting in a 21.51% decrease in AGPase activity and a 9.58% reduction in the endosperm starch content of transgenic maize. Moreover, the expressions of ZmBt1, ZmSSI, ZmSSIIa, and ZmSSIIIa were increased, while those of ZmSh2 and ZmSSIV reduced significantly in the endosperm of the transgenic maize. Overall, this study shows that ZmTCP7 functions as a transcriptional repressor of ZmBt2 and a negative regulator of endosperm starch accumulation, providing new insights into the regulatory networks that govern ZmBt2 expression and starch biosynthesis pathway in maize.

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Identification and functional analysis of the ICK gene family in maize

Cited by 6 publications

References 48 publications

Genome-Wide Analysis of the D-type Cyclin Gene Family Reveals Differential Expression Patterns and Stem Development in the Woody Plant Prunus mume

Genome-Wide Analysis of the D-type Cyclin Gene Family Reveals Differential Expression Patterns and Stem Development in the Woody Plant Prunus mume

Characterization of the soybean KRP gene family reveals a key role for GmKRP2a in root development

The novel ZmTCP7 transcription factor targets AGPase-encoding gene ZmBt2 to regulate storage starch accumulation in maize

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