Identification and functional analysis of a cytochrome P450 gene involved in imidacloprid resistance in Bradysia odoriphaga Yang et Zhang

Chen, Chengyu; Shan, Tisheng; Liu, Ying; Wang, Cuicui; Shi, Xueyan; Gao, Xiwu

doi:10.1016/j.pestbp.2018.11.009

Cited by 30 publications

(20 citation statements)

References 46 publications

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“…These genes are unique to insects and play important roles in the metabolism and detoxification of pesticides [22], and it has been suggested that overexpression of unigenes in these two families was involved in insecticide tolerance and detoxification in S. avenae. In B. odoriphaga, CYP6FV12 showed different fold changes in different life stages when exposed to imidacloprid and was confirmed to be related to B. odoriphaga resistance to imidacloprid [30], while CYP6CM1vQ was confirmed to be associated with a high level of imidacloprid resistance in Bemisia tabaci [31]. Four other upregulated unigenes were only differentially expressed following treatment with imidacloprid for 3 h, and all of four of these were annotated as the CYP6A subfamily gene, which indicated that this subfamily gene may be important to S. avenae detoxification to imidacloprid.…”

Section: Discussionmentioning

confidence: 78%

Transcriptome Analysis and Identification of Insecticide Tolerance-Related Genes after Exposure to Insecticide in Sitobion avenae

Wei

Zhong

Lin

et al. 2019

Genes

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Aphids cause serious losses to the production of wheat. The grain aphid, Sitobion avenae, which is the dominant species of aphid in all wheat regions of China, is resistant to a variety of insecticides, including imidacloprid and chlorpyrifos. However, the resistance and mechanism of insecticide tolerance of S. avenae are still unclear. Therefore, this study employed transcriptome analysis to compare the expression patterns of stress response genes under imidacloprid and chlorpyrifos treatment for 15 min, 3 h, and 36 h of exposure. S. avenae adult transcriptome was assembled and characterized first, after which samples treated with insecticides for different lengths of time were compared with control samples, which revealed 60–2267 differentially expressed unigenes (DEUs). Among these DEUs, 31–790 unigenes were classified into 66–786 categories of gene ontology (GO) functional groups, and 24–760 DEUs could be mapped into 54–268 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Finally, 11 insecticide-tolerance-related unigenes were chosen to confirm the relative expression by quantitative real-time polymerase chain reaction (qRT-PCR) in each treatment. Most of the results between qRT-PCR and RNA sequencing (RNA-Seq) are well-established. The results presented herein will facilitate molecular research investigating insecticide resistance in S. avenae, as well as in other wheat aphids.

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Section: Discussionmentioning

confidence: 78%

Transcriptome Analysis and Identification of Insecticide Tolerance-Related Genes after Exposure to Insecticide in Sitobion avenae

Wei

Zhong

Lin

et al. 2019

Genes

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“…The mechanisms of imidacloprid resistance in insects have been linked with a mutation in the target protein nicotinic acetylcholine receptor (nAChR) (Liu et al, 2005) and more commonly cytochrome P450-mediated detoxification (Karunker et al, 2008;Puinean et al, 2010;Hoi et al, 2014;Elzaki et al, 2017;Sun et al, 2018;Chen et al, 2019). In L. decemlineata, biochemical, physiological, and molecular biological studies showed that cytochrome-P450 mediated detoxification is the most common mechanism in neonicotinoid resistance (Nauen and Denholm, 2005;Alyokhin et al, 2008;Zhu et al, 2016), although the contributions from other mechanisms cannot be completely ruled out (Clements et al, 2016;Clements et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Silencing NADPH-Cytochrome P450 reductase affects imidacloprid susceptibility, fecundity, and embryonic development inLeptinotarsa decemlineata

Moural

Ban

Hernandez

et al. 2020

Preprint

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The Colorado potato beetle (CPB) is a prominent insect pest of potatoes, tomatoes and eggplants all over the world, however, the management of CPB remains a challenging task for more than one hundred years. We have successfully developed bacteria-expressed dsRNA-mediated feeding RNA interference (RNAi) approach in our previous study. A critical step towards field management of CPB via feeding RNAi is to identify effective and environmentally safe target genes. NADPH-Cytochrome P450 reductase (CPR) plays a central role in cytochrome P450 action. The full length Leptinotarsa decemlineata CPR (LdCPR) cDNA was isolated from an imidacloprid resistant population. The LdCPR gene was ubiquitously expressed in all stages tested but showed an increase in expression during the early stage of embryonic development. The bacteria-expressed dsRNA-mediated feeding RNAi of LdCPR in adults caused systemic knock down expression of the gene coding for LdCPR in both adults and their eggs. Suppression of LdCPR expression increased susceptibility of imidacloprid in resistant beetles, as well as a significant decrease of fecundity in female beetles (29% less eggs/day) and the hatching rate (47%) of their eggs. These data suggest that LdCPR plays important roles in insecticide detoxification and biosynthetic pathways of endogenous compounds and may serve as an essential target to control CPB.

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“…Based on the related products, it can be inferred that imidacloprid is degraded to produce 2-chloro-5-carboxy pyridine by hydroxylation and carbonylation, respectively. At the end of the three pathways, the pyridine ring undergoes dechlorination, and the final mineralization of imidacloprid is achieved [96][97][98][99][100][101].…”

Section: Degradation Of Imidacloprid By Physicochemical Methodsmentioning

confidence: 99%

“…According to real-time quantitative polymerase chain reaction (PCR), CYP6FV12 is the most expressed in the fourth instar larvae, which is 154.32 times higher than in eggs. When the concentration of imidacloprid increases, and the expression of CYP6FV12 also increases, and when this gene is knocked out, mortality increases by 28.62 [97]. Anti-imidacloprid Colorado potato beetle up-regulates the common cytochrome P450 gene (CYP6BJ a/b , CYP6BJ1v1, CYP9Z25, and CYP9Z29) by using the metabolic resistance of the exogenous transcription factors CncC and Maf to achieve the metabolic detoxification of potato plant allelopathic and imidacloprid [141].…”

Section: Molecular Biology Of Imidacloprid Degradationmentioning

confidence: 99%

Insights into the Toxicity and Degradation Mechanisms of Imidacloprid Via Physicochemical and Microbial Approaches

Pang

Lin

Zhang

et al. 2020

Toxics

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Imidacloprid is a neonicotinoid insecticide that has been widely used to control insect pests in agricultural fields for decades. It shows insecticidal activity mainly by blocking the normal conduction of the central nervous system in insects. However, in recent years, imidacloprid has been reported to be an emerging contaminant in all parts of the world, and has different toxic effects on a variety of non-target organisms, including human beings, due to its large-scale use. Hence, the removal of imidacloprid from the ecosystem has received widespread attention. Different remediation approaches have been studied to eliminate imidacloprid residues from the environment, such as oxidation, hydrolysis, adsorption, ultrasound, illumination, and biodegradation. In nature, microbial degradation is one of the most important processes controlling the fate of and transformation from imidacloprid use, and from an environmental point of view, it is the most promising means, as it is the most effective, least hazardous, and most environmentally friendly. To date, several imidacloprid-degrading microbes, including Bacillus, Pseudoxanthomonas, Mycobacterium, Rhizobium, Rhodococcus, and Stenotrophomonas, have been characterized for biodegradation. In addition, previous studies have found that many insects and microorganisms have developed resistance genes to and degradation enzymes of imidacloprid. Furthermore, the metabolites and degradation pathways of imidacloprid have been reported. However, reviews of the toxicity and degradation mechanisms of imidacloprid are rare. In this review, the toxicity and degradation mechanisms of imidacloprid are summarized in order to provide a theoretical and practical basis for the remediation of imidacloprid-contaminated environments.

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Identification and functional analysis of a cytochrome P450 gene involved in imidacloprid resistance in Bradysia odoriphaga Yang et Zhang

Cited by 30 publications

References 46 publications

Transcriptome Analysis and Identification of Insecticide Tolerance-Related Genes after Exposure to Insecticide in Sitobion avenae

Transcriptome Analysis and Identification of Insecticide Tolerance-Related Genes after Exposure to Insecticide in Sitobion avenae

Silencing NADPH-Cytochrome P450 reductase affects imidacloprid susceptibility, fecundity, and embryonic development inLeptinotarsa decemlineata

Insights into the Toxicity and Degradation Mechanisms of Imidacloprid Via Physicochemical and Microbial Approaches

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