Identification and functional characterization of the intermediate-conductance Ca²⁺-activated K⁺ channel (IK-1) in biliary epithelium

Identification and Functional Characterization of TMEM16A, a Ca2+-activated Cl− Channel Activated by Extracellular Nucleotides, in Biliary Epithelium

“…Mz-Cha-1, originally isolated from human cholangiocarcinoma cells exhibit phenotypic features of differentiated biliary epithelium (20,21) and have been utilized as models for purinergic signaling (22) and Ca 2ϩ -dependent secretion (23)(24)(25)(26). Mouse large cholangiocytes (MLCs) and mouse small cholangiocytes (MSCs) were isolated from normal mice (BALB/c) and immortalized by transfection with the SV40 large T antigen gene as described (27).…”

Endolymphatic Sodium Homeostasis by Extramacular Epithelium of the Saccule

“…Quayle et al (1997);5, Stocker (2004);6, Devor et al (1997) and Dutta et al (2009);7, Tanaka et al (2004);8, Duprat et al (19978, Duprat et al ( , 2005, Rajan et al (2000Rajan et al ( , 2005, and Morton et al (2005); 9, Safronov et al (1996), Cai et al (1998), andHadley et al (2000).…”

Physiology of Cholangiocytes

“…K + channels-Although Cl − channels are believed to provide the main driving force for biliary secretion, complementary actions by membrane K + channels are also required (72). In secretory epithelia, it is thought that activation of K + channels leads to membrane hyperpolarization by which the electrical driving force for continued Cl − efflux is maintained (72).…”

“…On the basolateral plasma membrane of human and rat cholangiocytes, two types of Ca 2+ -activated K + channels are expressed, SK2 and the intermediate-conductance Ca 2+ -activated K + channel 1, IK-1 (72,81,198). These two channels function in a complimentary manner and contribute to cholangiocyte secretory responses by hyperpolarizing the basolateral plasma membrane (72).…”