Identification and functional importance of plasma kallikrein in the synovial fluids of patients with rheumatoid, psoriatic, and osteoarthritis.

Abstract

Objectives-To determine and identify, unequivocally, if plasma kallikrein (PK) is present in the synovial fluid of patients with rheumatoid (RA), psoriatic (PA) and osteo (OA) arthritis, and to consider its functional importance in the inflamed joint. Methods-Therapeutically aspirated synovial fluids (pooled and individual samples, n = 66) were obtained from patients with arthritis. In addition, serum (n = 14) was collected from RA patients, and saliva (n = 10) and urine (n = 10) from normal individuals. Enzymic (amidase) and immunoreactive activities of PK and its precursor, prokallikrein (PPK), were determined. The presence of PK was assessed by incubation with soya bean trypsin inhibitor (SBTI), and by adsorption with anti-PK antibody linked to Sepharose. An enzyme-linked immunosorbant assay (ELISA) for PK was developed for quantitative measurement of total PK in biological fluids. Enhancement of the PK dose-response by RA synovial fluid made it necessary to remove RF from synovial fluids before determination of PK by ELISA. Results-Amidase activity was demonstrated in synovial fluid pools and shown to be inhibited completely by SBTI, and removed by prior treatment with anti-PK Sepharose. Total

Anti‐inflammatory synergy of MEN16132, a kinin B2 receptor antagonist, and dexamethasone in carrageenan‐induced knee joint arthritis in rats

“…Bradykinin and kallikrein activities have been found in the synovial fluid of patients affected by several arthropathies such as osteoarthritis, rheumatoid arthritis, gout and psoriatic arthritis. Moreover, kinin levels correlate well with the degree of joint disease (Rahman et al ., 1995; Nishimura et al ., 2002; Meini and Maggi, 2008). Bradykinin potentially affects the joint at the level of either synovium, cartilage or bone, as synovial cells, chondrocytes and osteoblasts all express the kinin B 2 receptor.…”

Augmented plasma and tissue kallikrein like activity in synovial fluid of patients with inflammatory articular diseases

“…Therefore, in this study, we compared the activities of kallikreins and kininogen measured simultaneously in plasma and synovial fluid from patients with inflammatory joint diseases. Recently, Rahman et al [8] elegantly identified the presence of total kallikrein and active plasma kallikrein in synovial fluid of patients with rheumatoid arthritis, psoriatic arthritis and osteoarthrosis. In our study, besides demonstrating the presence of amidase activity in synovial fluid we also observed increased levels in relation to plasma.…”

“…Total kininogen was studied in plasma and synovial fluid of patients with adult rheumatoid arthritis, reporting diminution of levels of HKg in synovial fluid of these patients [4]. However, plasma and tissue kallikreins have been detected separately in plasma or synovial fluid of patients with arthritis [5][6][7][8]. In this study, we compared the amidase activity of plasma and tissue kallikreins upon chromogenic substrates and kininogen levels simultaneously in plasma and synovial fluid of patients with inflammatory articular diseases.…”

Influence of interleukin-1β on bradykinin-induced responses in guinea pig peritoneal macrophages

“…Bradykinin plays an important role in the pathogenesis of inflammatory disease, such as rheumatoid arthritis, asthma and rhinitis [1][2][3]. Additionally, a possible role for kinins has been postulated in progressive neurodegenerative diseases such as multiple sclerosis by the transformation of the resting microglia into an active state with a macrophage-like activity [4].…”