Identification and gene mapping of a panicle apical abortion mutant (<italic>paa1331</italic>) in rice

Abstract

部一次枝梗退化 [1]. 目前克隆的控制穗顶部退化的基 因有PAA2(panicle apical abortion2)、TUT1(tutou1)、 OsALMT7(aluminum-activated malate transporter)、 SPL6(squamosal promoter-binding protein-like) [2~6] .

A putative SUBTILISIN-LIKE SERINE PROTEASE 1 (SUBSrP1) regulates anther cuticle biosynthesis and panicle development in rice

“…Due to the further unavailability of InDel markers in this region, we performed a MutMap assay to find any mutation in the primary mapped region by following a method of Abe et al [58] . MutMap whole genome sequencing (Illumina HiSeq 2500 platform) revealed an SNP index of 1 due to the presence of a single nucleotide mutation in ( Os04g0483400 ) LOC_Os04g40720 [52] . Consistent with the primary mapping by SSR markers, Mutmap results showed an SNP (A > G) located between the previously mapped region of SSR markers RM252 and RM5979.…”

A SUBTILISIN-LIKE SERINE PROTEASE1 (OsSUBSrP1), plays an important role in the wax and cutin pathway, is essential for panicle development in rice

“…Current study presents the further genetic analysis and functional validation of our previous preliminary study of a gene mapped on chromosome 4 controlling panicle abortion phenotype (Hou et al ., 2018). However, the its further follow up genetic analysis and functional analysis was not carried out.…”

“…In F 1 generations of both populations, all plants did not show degeneration phenotype. Genetic analysis of these population revealed that a single recessive gene controls the phenotype of panicle degeneration in apa1331(Hou et al, 2018) .…”

“…Primary mapping of apa1331 revealed that the candidate gene resides between the SSR marker 4-9 and 4-10 on the short arm of chromosome 4 (Figure 6A). The gene was narrowed down between region of RM252 and RM5979 markers in a distance of 16.4cM (Hou et al ., 2018). Due to the further unavailability of InDel markers in this region, we performed MutMap assay to find any mutation in the primary mapped region (Abe et al ., 2012).…”

“…Due to the further unavailability of InDel markers in this region, we performed MutMap assay to find any mutation in the primary mapped region (Abe et al ., 2012). MutMap whole genome sequencing (Illumina HiSeq 2500 platform) revealed an SNP index of 1 due to the presence of an SNP in LOC_Os04g40720 (Hou et al, 2018). Consistent with the primary mapping by SSR markers, Mutmap results showed an SNP (A > G) was located between the previously mapped region between RM252 and RM5979.…”