Identification and Genetic Characterization of Mycoplasma Species Affecting Respiratory System in Egyptian Cattle

El-Tawab, Ashraf Awad Abd; Hassan, N.; Ramadan, Mohamed

doi:10.21608/bvmj.2021.67213.1361

Cited by 3 publications

(3 citation statements)

References 20 publications

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“…(32%) among different samples' types obtained from the respiratory tracts of cattle in Sharkia Governorate, Egypt. This result is partially similar to other studies conducted in Europe (34.3%) [54] and Turkey (35.4%) [55], and it was higher than the results of a previous study conducted in Nigeria (7.7%) [56] but lower than those results observed in other studies conducted in the USA (92%) [57] and Egypt (67.5%) [58]. Our findings demonstrated a high incidence of bovine Mycoplasma spp.…”

Section: Discussionsupporting

confidence: 87%

Prevalence and Antimicrobial Susceptibility of Bovine Mycoplasma Species in Egypt

Ammar

El-Hamid

Mohamed

et al. 2022

Biology

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Among many bovine Mycoplasma species (spp.), Mycoplasma bovis is recognized as a significant causative agent of respiratory diseases in cattle. In recent years, resistant M. bovis isolates, especially to fluoroquinolones, have been reported globally as a result of the extensive usage of antimicrobials in the treatment of bovine pneumonia. Therefore, the aim of this study is to investigate the prevalence and antimicrobial susceptibility patterns of bovine Mycoplasma spp. isolated from the respiratory tracts of cattle in Egypt and to assess the fluoroquinolones resistance in the recovered mycoplasma isolates via broth microdilution and conventional PCR techniques. Conventional phenotypic methods identified 128 mycoplasma isolates (32%) from 400 different samples, with M. bovis being the predominant spp. (61%), followed by M. bovirhinis (15%). Of note, mycoplasma isolates were rarely isolated from total healthy lung tissues (7/55, 12.7%), but they were frequently isolated from pneumonic lungs (31/45, 68.9%). All the examined mycoplasma isolates (n = 76) were sensitive to tilmicosin, tylosin, tulathromycin, spiramycin, and spectinomycin (100% each), while 60.5% and 43.4% of the examined isolates had high minimum inhibitory concentration (MIC) values to enrofloxacin and doxycycline, respectively. Three and two mycoplasma isolates with high enrofloxacin MICs were confirmed to be M. bovis and M. bovirhinis, respectively, by PCR assays. All molecularly confirmed mycoplasma isolates (n = 5) were positive for the gyrA gene (100%); meanwhile, three isolates (60%) were positive for the parC gene. In conclusion, our findings revealed alarming resistance to enrofloxacin and doxycycline antibiotics; thus, antimicrobial usage must be restricted and molecular techniques can help in the rapid detection of the resistant strains.

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Section: Discussionsupporting

confidence: 87%

Prevalence and Antimicrobial Susceptibility of Bovine Mycoplasma Species in Egypt

Ammar

El-Hamid

Mohamed

et al. 2022

Biology

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“…These results are lower than other studies addressing the prevalence of M. bovis in Iraq, Mhmood and Rhaymah, [14] and Hamad et al [15] who were mentioned that the popularity of M. bovis in calves in Mosul city were 76.09% and 86.5% using i-ELISA test and PCR techniques, respectively. There are several studies that have detected the prevalence of M. bovis in cattle in various countries, including Turkey, was 23.3% using direct fluorescent antibody testing (DFAT) [27], In Iran, was 88% using the nested PCR (n-PCR) technique [28], in Jordan, was 27.3% using conventional PCR (c-PCR) technique [29], in Saudi Arabia, was 24% using c-PCR technique [30], in China, was 48.7% using i-ELSA [31], in Egypt, was 67.5% and 8.3%, using culture method and c-PCR technique respectively [32,33], in Sudan, was 7.2% using i-ELISA test [34], in Algeria, was 69.0% and 58.0% using i-ELISA test and Real time PCR technique respectively [35], in United States of America, was 100% and 87.5% using LAMP and real-time PCR (RT-PCR) technique respectively [36], in Brazil, was 91.4%, 1.1% and 62.3% using (IHC), RT-PCR technique, and i-ELISA test respectively [1,37,38], and in Australia, was 42.5% using i-ELISA test [39]. The differs in the prevalence of M. bovis among counties may be due to different management approaches, environmental conditions, efficient diagnostic techniques, types of samples that were tested, and the presence or absence of additional factors, such as the host's age, physical characteristics, and immunological status.…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Mycoplasma bovis in Cattle in Nineveh Governorate, Iraq

Al-Azow,

Alsarhan,

Hamad

2025

Egyptian Journal of Veterinary Sciences

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T HE CURRENT STUDY aimed to determine the prevalence of Mycoplasma bovis in cattle in Nineveh Governorate, Iraq, using the culture method, indirect enzyme-linked immunosorbent assay (i-ELISA), and conventional polymerase chain reaction (c-PCR) technique, to evaluate the efficiency of the various laboratory methods used in this study. During the period from March 2022 to February 2023, a total of 352 nasal swabs were collected randomly from cattle and tested using the culture method and conventional polymerase chain reaction (c-PCR) technique (amplifying the uvrC gene) for isolation and molecular detection of M. bovis , respectively. Moreover, 352 blood samples were collected from the same animals for the detection of antibodies against M. bovis using i-ELISA. The results observed that the prevalence of M. bovis was 23.57% (83 out of 352) using the culture method, 30.68% (108 out of 352) using the c-PCR technique, and 47.15% (166 out of 352) using i-ELISA. According to the Kappa value (0.807), there was perfect agreement between the c-PCR technique and the culture method, with sensitivity, specificity, and accuracy were 98.79%, 90.33%, and 92.32% for c-PCR technique respectively. While there was moderate agreement between i-ELISA test and the culture method based on Kappa (0.444), with sensitivity, specificity and accuracy were 89.15%, 65.79%, and 71.30% for i-ELISA, respectively. This study was concluded that M. bovis is prevalent in Nineveh Governorate and the PCR technique is the more efficient tool than ELISA for detection of M. bovis in suspected samples.

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“…In the present study, uvrC (22.2%) was the most reported gene, followed by oppD (1.7%) and 16S-23S rRNA ITS gene (1.4%). Moreover, other M. bovis genes were reported in studies that successfully diagnosed bovine mastitis using a conventional singleplex PCR [39,48,64,75,79,85,90].…”

Section: Conventional Pcrmentioning

confidence: 99%

A Systematic Review of the Recent Techniques Commonly Used in the Diagnosis of Mycoplasma bovis in Dairy Cattle

Okella,

Tonooka,

Okello

2023

Pathogens

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Early detection of Mycoplasmal mastitis is greatly hampered by late seroconversion, slow growth of Mycoplasma organisms, intermittent shedding, and the high cost of diagnostic tests. To improve future diagnostic development, examining the available techniques is necessary. Accordingly, the present study systematically reviewed M. bovis diagnostic studies published between January 2000 and April 2023 utilizing the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) protocol. The protocol registration was performed according to the Open Science Framework (osf.io/ug79h), and the electronic search was conducted in the World Catalog, Mendeley, ProQuest, ScienceDirect, Semantic Scholar, PubMed, Google Scholar, Prime Scholar, and PubMed Central databases using a Boolean operator and inclusion and exclusion criteria. Of the 1194 pieces of literature retrieved, 67 studies were included. Four broad categories of up to 16 diagnostic approaches were reported: microbial culture, serological, DNA-based, and mass spectrometry. Overall, DNA-based techniques were the most published (48.0%), with recombinase polymerase amplification (RPA) and loop-mediated isothermal amplification (LAMP) as the most promising user-friendly, equipment-free techniques. On the other hand, mass spectrometry was reported as the least utilized (2.9%) given the high equipment cost. Though costly and laboratory-allied, DNA-based techniques, particularly PCRs, were reported as the most rapid and specific approach.

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Identification and Genetic Characterization of Mycoplasma Species Affecting Respiratory System in Egyptian Cattle

Cited by 3 publications

References 20 publications

Prevalence and Antimicrobial Susceptibility of Bovine Mycoplasma Species in Egypt

Prevalence and Antimicrobial Susceptibility of Bovine Mycoplasma Species in Egypt

Prevalence of Mycoplasma bovis in Cattle in Nineveh Governorate, Iraq

A Systematic Review of the Recent Techniques Commonly Used in the Diagnosis of Mycoplasma bovis in Dairy Cattle

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