Identification and genetic characterization of a new totivirus from Bursera graveolens in western Ecuador

Abstract

The complete genomic sequence of a previously uncharacterized virus provisionally named Bursera graveolens totivirus 1 (BgTV-1) was obtained from Bursera graveolens (Kunth) Triana & Planch., a tree known as "palo santo" in Ecuador. The BgTV-1 genome is monopartite double-stranded RNA (dsRNA) and 4,794 nucleotides (nt) long (GenBank accession number ON988291). Phylogenetic analysis of the capsid protein (CP) and RNA-dependent-RNA-polymerase (RdRp) placed BgTV-1 in a clade with other plantassociated totiviruses. Amino acid (aa) sequence comparisons of BgTV-1 putative proteins showed the highest identities to those of taro-associated totivirus L (QFS21890.1-QFS21891.1) and Panax notoginseng virus A (YP_009225664.1-YP_009225665.1) with 51.4% and 49.8%, for the CP, and 56.4%

Transcriptome Analysis Reveals a Diverse Range of Novel Viruses in Australian Sugarcane Soldier Fly (Inopus flavus) Larvae

“…In recent years, novel totiviruses have been detected in arthropods from Europe [ 23 ], Australia [ 45 , 46 ], Asia [ 47 ] and South America [ 48 ]. Totiviruses have also been detected in plants worldwide, notably in Australia [ 49 ], China [ 50 ] and Ecuador [ 51 ].…”

Current epidemiological situation of mealybug wilt of pineapple disease in Ecuador

“…Total RNA extraction and RT‐PCR for virus testing was done as described by Cornejo‐Franco et al. (2023) using primers and PCR conditions detailed by Larrea‐Sarmiento et al. (2022) and Alvarez‐Quinto et al.…”