Identification and genetic characterization of an Aegilops tauschii ortholog of the wheat leaf rust disease resistance gene Lr1

Ling, Hong‐Qing; Qiu, Jiwen; Singh, Ravi P.; Keller, Beat

doi:10.1007/s00122-004-1734-5

Cited by 27 publications

(19 citation statements)

References 23 publications

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“…Lr1 is located on chromosome 5D of wheat and Ling et al . () screened > 200 accessions of Ae. tauschii to search for an Lr1 ‐type of gene in the D‐genome donor of bread wheat.…”

Section: Nlr Evolution In Triticeaementioning

confidence: 99%

Molecular genetics and evolution of disease resistance in cereals

2016

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Contents 320I.320II.321III.321IV.322V.324VI.328VII.329330References330 Summary Cereal crops produce a large part of the globally consumed food and feed. Because of the constant presence of devastating pathogens, the molecular characterization of disease resistance is a major research area and highly relevant for breeding. There has been recent and accelerating progress in the understanding of three distinct resistance mechanisms in cereals: resistance conferred by plasma membrane‐localized receptor proteins; race‐specific resistance conferred by intracellular immune receptors; and quantitative disease resistance. Intracellular immune receptors provide a particularly rich source for evolutionary studies, and have, for example, resulted in the recent discovery of a novel detection mechanism based on integrated decoy domains. Evolutionary studies have also revealed the origins of active resistance genes in both wild progenitors of today's cereals as well as in cultivated forms. In addition, independent evolution of orthologous genes in related cereals has resulted in resistance to different pathogen species. Quantitative resistance genes have been best characterized in wheat. The quantitative resistance genes identified so far in wheat encode transporter proteins or unusual kinase proteins. The recent discoveries in these three different resistance mechanisms have contributed to the basic molecular understanding of cereal immunity against pathogens and have suggested novel applications for resistance breeding.

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“…Lr1 is located on chromosome 5D of wheat and Ling et al . () screened > 200 accessions of Ae. tauschii to search for an Lr1 ‐type of gene in the D‐genome donor of bread wheat.…”

Section: Nlr Evolution In Triticeaementioning

confidence: 99%

Molecular genetics and evolution of disease resistance in cereals

2016

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“…Xpsr567 is an RFLP marker previously mapped to the long arm of chromosome 5D (Xie et al 1993) and shown to be closely linked to Lr1 Gallego et al 1998;Ling et al 2003Ling et al , 2004. Partial resistance gene analog (RGA) sequences 567A (AY123939), 567B (AY123940), and 567C (AY123941) were obtained by sequencing of Aegilops tauschii BAC clones identified with a psr567 probe (Ling et al 2003).…”

Section: Plant Populations and Phenotypic Assessmentmentioning

confidence: 99%

“…A high-resolution map was later published by Ling et al (2003) locating the gene within a 0.16 cM region. This gene is putatively orthologous to a gene in A. tauschii which confers the same resistance phenotype to a number of rust races (Ling et al 2004). A high-density genetic map of the Lr1 locus was constructed and aligned with a physical map obtained by screening the Glenlea BAC library.…”

Section: Introductionmentioning

confidence: 99%

Leaf rust resistance gene Lr1, isolated from bread wheat (Triticum aestivum L.) is a member of the large psr567 gene family

et al. 2007

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In hexaploid wheat, leaf rust resistance gene Lr1 is located at the distal end of the long arm of chromosome 5D. To clone this gene, an F(1)-derived doubled haploid population and a recombinant inbred line population from a cross between the susceptible cultivar AC Karma and the resistant line 87E03-S2B1 were phenotyped for resistance to Puccinia triticina race 1-1 BBB that carries the avirulence gene Avr1. A high-resolution genetic map of the Lr1 locus was constructed using microsatellite, resistance gene analog (RGA), BAC end (BE), and low pass (LP) markers. A physical map of the locus was constructed by screening a hexaploid wheat BAC library from cultivar Glenlea that is known to have Lr1. The locus comprised three RGAs from a gene family related to RFLP marker Xpsr567. Markers specific to each paralog were developed. Lr1 segregated with RGA567-5 while recombinants were observed for the other two RGAs. Transformation of the susceptible cultivar Fielder with RGA567-5 demonstrated that it corresponds to the Lr1 resistance gene. In addition, the candidate gene was also confirmed by virus-induced gene silencing. Twenty T (1) lines from resistant transgenic line T (0)-938 segregated for resistance, partial resistance and susceptibility to Avr1 corresponding to a 1:2:1 ratio for a single hemizygous insertion. Transgene presence and expression correlated with the phenotype. The resistance phenotype expressed by Lr1 seemed therefore to be dependant on the zygosity status. T (3)-938 sister lines with and without the transgene were further tested with 16 virulent and avirulent rust isolates. Rust reactions were all as expected for Lr1 thereby providing additional evidence toward the Lr1 identity of RGA567-5. Sequence analysis of Lr1 indicated that it is not related to the previously isolated Lr10 and Lr21 genes and unlike these genes, it is part of a large gene family.

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“…However, wild species are most commonly used as donors of disease resistance genes (Jan et al 2002, 2004a, b; Kuhl et al 2001; Ling et al 2004). The dominant monogenic locus Pl ARG originated from the wild species H. argophyllus and is an outstanding source of resistance, because of broad-spectrum resistance against all known races of P. halstedii .…”

Section: Discussionmentioning

confidence: 99%

Fine mapping of the sunflower resistance locus Pl ARG introduced from the wild species Helianthus argophyllus

et al. 2010

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Downy mildew, caused by Plasmopara halstedii, is one of the most destructive diseases in cultivated sunflower (Helianthus annuus L.). The dominant resistance locus PlARG originates from silverleaf sunflower (H. argophyllus Torrey and Gray) and confers resistance to all known races of P. halstedii. We mapped PlARG on linkage group (LG) 1 of (cms)HA342 × ARG1575-2, a population consisting of 2,145 F2 individuals. Further, we identified resistance gene candidates (RGCs) that cosegregated with PlARG as well as closely linked flanking markers. Markers from the target region were mapped with higher resolution in NDBLOSsel × KWS04, a population consisting of 2,780 F2 individuals that does not segregate for PlARG. A large-insert sunflower bacterial artificial chromosome (BAC) library was screened with overgo probes designed for markers RGC52 and RGC151, which cosegregated with PlARG. Two RGC-containing BAC contigs were anchored to the PlARG region on LG 1.Electronic supplementary materialThe online version of this article (doi:10.1007/s00122-010-1416-4) contains supplementary material, which is available to authorized users.

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Identification and genetic characterization of an Aegilops tauschii ortholog of the wheat leaf rust disease resistance gene Lr1

Cited by 27 publications

References 23 publications

Molecular genetics and evolution of disease resistance in cereals

Molecular genetics and evolution of disease resistance in cereals

Leaf rust resistance gene Lr1, isolated from bread wheat (Triticum aestivum L.) is a member of the large psr567 gene family

Fine mapping of the sunflower resistance locus Pl ARG introduced from the wild species Helianthus argophyllus

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