Identification and genetic characterization of two porcine astroviruses from domestic piglets in China

Li, Jinsong; Li, Mao-zhong; Zheng, Lei; Liu, Na; Li, Dan-di; Duan, Zhaojun

doi:10.1007/s00705-015-2569-x

Cited by 22 publications

(13 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous studies have reported the existence of multiple PAstV genotypes in China. For example, PAstV-1 was identified in Shanghai as well as the provinces of Hunan and Guangxi ( 22 , 25 ), while PAstV-3 was identified in Guangxi province only ( 22 ), PAstV-4 has been reported in the provinces of Tianjin, Hunan, and Guangxi ( 22 , 24 , 25 ), and PAstV-5 has been detected in the provinces of Hebei, Sichuan, Hunan, and Guangxi ( 11 , 22 , 24 – 26 ). In the present study, three PAstV-4 strains were identified in the provinces of Jilin, Shanxi, and Jiangxi, and two PAstV-5 strains were identified in Shandong and Jiangxi provinces, respectively.…”

Section: Resultsmentioning

confidence: 99%

Coinfection and Genetic Characterization of Porcine Astrovirus in Diarrheic Piglets in China From 2015 to 2018

Yang

et al. 2020

Front. Vet. Sci.

View full text Add to dashboard Cite

Porcine astrovirus (PAstV) is broadly distributed globally and exists as at least five distinct genotypes. PAstV, which was recently identified as an important pathogen of diarrhea in piglets, is widely distributed in China. However, few studies have investigated the coinfection and genetic characterization of PAstV in diarrheic piglets in China. In this study, 89 PAstV-positive samples were identified in 543 diarrhea samples in China from 2015 to 2018, of which 75.28% (67/89) were coinfected with three to five different porcine pathogens, while none were positive for PAstV only. Among the 543 diarrhea samples, statistical analysis showed that PAstV-induced diarrhea was potentially associated with coinfection of PEV (p < 0.01) and GARV (p < 0.01). Phylogenetic analysis showed that the 27 identified PAstV strains belong to three different genotypes and that PAstV-2 (81.48%, 22/27) was predominant in diarrheic piglets in China, followed by PAstV-4 (11.11%, 3/27) and PAasV-5 (7.41%, 2/27). Sequence analysis revealed that the 27 RdRp genes identified in this study had nucleotide homologies of 53.8-99.5%. In addition, the RdRp gene of PAstV-4 strain JL/MHK/2018/0115 harbored a unique insert of three nucleotides (GAA) as compared with other known PAstV-4 strains. Furthermore, the genotypes of PAstV varied among different geographical locations, although PAstV-2 was the most widely distributed in China. These data demonstrate that PAstV coinfection with other porcine pathogens was common and there was genetic diversity of PAstV in diarrheic piglets in China.

show abstract

Section: Resultsmentioning

confidence: 99%

Coinfection and Genetic Characterization of Porcine Astrovirus in Diarrheic Piglets in China From 2015 to 2018

Yang

et al. 2020

Front. Vet. Sci.

View full text Add to dashboard Cite

show abstract

“…In China, the knowledge for PAstV is still limited, with only four studies reporting the identification and distribution of PAstV. Specifically, a PAstV sequence belonging to PAstV2 (HQ647383) has been identified in domestic pigs with diarrhea in 2009 [12], a PAstV1 (GQ914773) strain was described in healthy domestic pigs in 2008 [29], and a PAstV2 (KP747573) sequence and a PAstV5 (KP747574) sequence were obtained from healthy domestic piglets [15]. Very recently, several PAstV2 and PAstV5 sequences were identified in domestic pigs or wild boars in the Sichuan province, China [8].…”

Section: Introductionmentioning

confidence: 99%

Identification and characterization of multiple porcine astrovirus genotypes in Hunan province, China

Xiao

Luo

et al. 2016

Arch Virol

View full text Add to dashboard Cite

Astroviruses (AstVs) can infect a variety of hosts, including mammalian and avian species, and are commonly associated with enteric infections. Recently, mammalian AstVs have been linked to extra-intestinal manifestations, including neurologic disorders in humans, cattle and minks, demonstrating zoonotic potential. So far, five porcine AstV (PAstV) genotypes have been identified, with PAstV1, PAstV2, PAstV3 and PAstV5 implicated in cross-species transmission. Our knowledge about PAstV epidemiology in China is still limited. In this study, two duplex differential RT-PCR assays were developed to investigate the distribution and prevalence of PAstV1, PAstV2, PAstV4 and PAstV5. Two hundred eighteen samples were collected from 33 farms and pigs with known diarrhea status in nine regions of Hunan province in China. Specifically, 126 small intestines, 51 fecal swabs, 20 lungs, 19 spleens and two kidneys were obtained. PAstVs were detected in all nine regions and in 81.8% (27/33) of the pig farms investigated. The overall prevalence of PAstV was 46.3% (101/218), with PAstV5 as the predominant type, with a positive rate of 24.8% (54/218). The prevalence of PAstV4, PAstV1 and PAstV2 was 16.1% (35/218), 14.7% (32/218) and 10.1% (22/218), respectively. Besides being present in intestines and fecal swabs, PAstV RNA was also detected in lungs, spleens and kidneys. Sequencing revealed a high level of genetic divergence within each genotype, and a higher positive rate of PAstV5 was associated with pigs with diarrhea compared to pigs without diarrhea. This study revealed for the first time that PAstV4 is circulating in China, and that PAstV5 is the dominant genotype in pig herds in Hunan province in China.

show abstract

“…2 ), suggesting that it is a novel CAstV lineage. Importantly, except for the group of Mamastrovirus 5/CAstV, there was higher similarity with MAstV3 for PoAstV5 (87.6–98.3% amino acid identity) and PoAstV2 (84.3–100% amino acid identity), which belonged to novel genotypes circulating in pig population in China [ 20 , 21 ]. This is the first report that showed CAstVs share higher homology with other lineage, suggesting that there may be potential recombination events in CAstVs infection.…”

mentioning

confidence: 99%

Detection and genetic characterization of canine astroviruses in pet dogs in Guangxi, China

Zhou

Liu

et al. 2017

Virol J

View full text Add to dashboard Cite

BackgroundAstroviruses (AstVs) have been reported to infect and cause gastroenteritis in most animal species. Human AstVs were regarded the causative agent of viral diarrhea in children. In dogs, little is known about the epidemiology and clinical significance of AstV infection.FindingsIn this study, we collected and tested 253 rectal swabs from pet dogs; of which 64 samples (25.3%) tested positive for AstVs with diarrhea and 15 more samples (5.9%) also was identified as AstVs, however without any clinical signs. Phylogenetic analysis of 39 partial ORF1b sequences from these samples revealed that they are similar to AstVs, which can be subdivided into three lineages. Interestingly, out of the 39 isolates sequenced, 16 isolates are shown to be in the Mamastrovirus 5/canine astrovirus (CAstV) lineage and the remaining 23 isolates displayed higher similarities with known porcine astrovirus (PoAstV) 5 and 2. Further, analysis of 13 capsid sequences from these isolates showed that they are closely clustered with Chinese or Italy CAstV isolates.ConclusionsThe findings indicate that CAstVs commonly circulate in pet dogs, and our sequencing results have shown the genomic diversity of CAstVs leading to increasing number of clusters.

show abstract

Identification and genetic characterization of two porcine astroviruses from domestic piglets in China

Cited by 22 publications

References 17 publications

Coinfection and Genetic Characterization of Porcine Astrovirus in Diarrheic Piglets in China From 2015 to 2018

Coinfection and Genetic Characterization of Porcine Astrovirus in Diarrheic Piglets in China From 2015 to 2018

Identification and characterization of multiple porcine astrovirus genotypes in Hunan province, China

Detection and genetic characterization of canine astroviruses in pet dogs in Guangxi, China

Contact Info

Product

Resources

About