Identification and genetic characterization of porcine circovirus type 3 in China

Porcine circovirus 3 ( PCV 3) was recently discovered and is a new species of the genus circovirus. Clinically, it is associated with absence of symptoms or with different clinical syndromes. It has been reported in different countries of America, Europe and Asia. Last year, in Colombia, some farms have reported symptoms similar to those caused by PCV 2. Samples were taken from two farms located in the centre of the country, and the presence of PCV 3 was determined by PCR in two samples, one from a pool of sera and another from mesenteric lymph node. The strains were fully sequenced (GenBank accession numbers MH 327784 and MH 327785) and classified into subgroups a1 and a2. According to this classification and its analysis, strain a2 is located within the group called “Linker” that may be evolving towards group “b”. In addition to the above, the two Colombian strains were compared with 104 strains reported in the GenBank database. The phylogenetic tree obtained grouped according to the classification of subgroups a1, a2, b1 and b2. It was found that subgroups a1 and a2 were well grouped when comparing whole genomes, but the same was not observed with the strains of group “b”. In the latter, no subgroups were evidenced when comparing complete genomes. It is suggested that a new classification of PCV 3 subgroups should be proposed, based on whole genome sequences. This is the first report of PCV 3 in Colombia and its complete genome sequence.

show abstract

“…All of these strains were grouped into PCV3 group 2, according to (Ku et al . ), and into group “a”, according to (Fux et al . ).…”

Section: Resultsmentioning

confidence: 99%

First detection of porcine circovirus type 3 in Colombia and the complete genome sequence demonstrates the circulation of PCV3a1 and PCV3a2

Vargas-Bermúdez

Campos

Bonil

et al. 2019

Veterinary Medicine & Sci

View full text Add to dashboard Cite

show abstract

“…It was reported that PCV3 was detected in fetuses and it indicated that PCV3 could spread by vertical transmission (Ku et al . ). In this study, sequencing of the partials capsid gene revealed a high homology among the investigated strains and they also showed high homology with the fragment of CAP PCV3/29160.…”

Section: Discussionmentioning

confidence: 97%

“…The earliest case could be retrospective to the clinical sample collected in 2014 (Ku et al . ; Fan et al . ; Zheng et al .…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Establishment of a sensitive TaqMan‐based real‐time PCR assay for porcine circovirus type 3 and its application in retrospective quarantine of imported boars to China

Chen

Wang

Zhang

et al. 2019

Veterinary Medicine & Sci

View full text Add to dashboard Cite

Porcine circovirus type 3 ( PCV 3) is a novel pathogen first identified in the United States in 2016. As there is a high possibility that no clinical signs of infection are observed in the host, an accurate and sensitive method is needed for quarantine on numerous live pigs especially for international pig trade. In this study, a TaqMan‐based real‐time PCR assay specifically for PCV 3 was established without cross‐reactions with other non‐targeted pig viruses. The sensitivity of the current approach is about 1.5 × 10 1 copies μ L −1 plasmid DNA while the sensitivity of the conventional PCR is about 1.5 × 10 2 copies μ L −1 plasmid DNA . Further, this assay was applied in the retrospective quarantine on serum samples of 601 commercial live boars imported to China from the United States, France and the United Kingdom from 2011 to 2017. The results revealed that PCV 3 could be detected positive in the commercial boars imported from the United States and the above‐mentioned western European countries and phylogenetic study also revealed that viral isolates were grouped with some isolates from Korea and the United States. Our study suggested that PCV 3 may be prevalent globally since 2011.

show abstract

“…However, PCV‐3 detection in the presence of different disease conditions such as porcine dermatitis and nephropathy syndrome, reproductive and respiratory disorders (Ku et al . ; Palinski et al . ; Shen et al .…”

Section: Introductionmentioning

confidence: 99%

Diagnostic accuracy of two DNA‐based molecular assays for detection of porcine circovirus 3 in swine population using Bayesian latent class analysis

Franzo

Segalés

Klaumann

et al. 2019

Lett Appl Microbiol

View full text Add to dashboard Cite

Molecular‐based tools sometimes are the only laboratory techniques available to detect a recently discovered agent, and their validation without the existence of previously described ‘gold standard’ methods poses a challenge for the diagnosticians. A good example within this scenario is the recently described porcine circovirus 3 (PCV‐3) in the swine population worldwide, from which only few PCR methods have been described. Therefore, the primary objective of this study was to estimate the diagnostic accuracy of a direct PCR (dPCR) and a real‐time qPCR (qPCR) for detection of PCV‐3 in Italian swine population. Bayesian latent class analysis approach was used to rigorously assess their features and applicability in routine diagnostic activity. Data on dPCR and qPCR were available from 116 domestic pigs, which were randomly selected from 55 farms located at different regions in Northern Italy. The sensitivity (Se) estimates of dPCR (94%; posterior credible interval (PCI%) 84–100) and qPCR (96%; PCI% 90–100) were high and similar. The estimated specificity (Sp) of both dPCR and qPCR assays was around 97%. dPCR and qPCR assays showed a high and comparable Se and Sp estimates for the detection of PCV‐3 in Italian swine population. Significance and Impact of the Study The continuous discovery of new pathogens poses a challenge in the development and evaluation of adequate diagnostic tools. In fact, since molecular‐based tools sometimes are the only available laboratory techniques, it is typically difficult to evaluate their diagnostic performances in the absence of a gold standard. The present study assesses this issue, demonstrating the excellent performances of two PCR‐based assays for porcine circovirus 3 (PCV‐3) detection using a Bayesian latent class analysis approach. Therefore, the molecular tests evaluated under this study constitute reliable tools for the routine diagnosis and surveillance programs of PCV‐3 circulating in swine populations.

show abstract

Identification and genetic characterization of porcine circovirus type 3 in China

Cited by 192 publications

References 19 publications

First detection of porcine circovirus type 3 in Colombia and the complete genome sequence demonstrates the circulation of PCV3a1 and PCV3a2

First detection of porcine circovirus type 3 in Colombia and the complete genome sequence demonstrates the circulation of PCV3a1 and PCV3a2

Establishment of a sensitive TaqMan‐based real‐time PCR assay for porcine circovirus type 3 and its application in retrospective quarantine of imported boars to China

Diagnostic accuracy of two DNA‐based molecular assays for detection of porcine circovirus 3 in swine population using Bayesian latent class analysis

Contact Info

Product

Resources

About